Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_5688 and Pf6N2E2_5689 are separated by 92 nucleotidesPf6N2E2_5689 and Pf6N2E2_5690 are separated by 47 nucleotidesPf6N2E2_5690 and Pf6N2E2_5691 are separated by 172 nucleotidesPf6N2E2_5691 and Pf6N2E2_5692 are separated by 78 nucleotides Pf6N2E2_5688 - FIG00955605: hypothetical protein, at 6,502,895 to 6,503,641 _5688 Pf6N2E2_5689 - Flagellar biosynthesis protein FlgN, at 6,503,734 to 6,504,201 _5689 Pf6N2E2_5690 - Negative regulator of flagellin synthesis FlgM, at 6,504,249 to 6,504,566 _5690 Pf6N2E2_5691 - Flagellar basal-body P-ring formation protein FlgA, at 6,504,739 to 6,505,503 _5691 Pf6N2E2_5692 - Chemotaxis protein CheV (EC 2.7.3.-), at 6,505,582 to 6,506,508 _5692
Group Condition Pf6N2E2_5688 Pf6N2E2_5689 Pf6N2E2_5690 Pf6N2E2_5691 Pf6N2E2_5692
motility inner cut, LB soft agar motility assay +0.0 -4.7 N.D. -5.3 +1.7
motility outer cut, LB soft agar motility assay -0.1 -5.0 N.D. -3.5 +1.4
motility inner cut, LB soft agar motility assay +0.4 -4.9 N.D. -5.1 +2.8
motility outer cut, LB soft agar motility assay +0.1 -2.6 N.D. -4.2 +1.4
motility inner cut, LB soft agar motility assay +0.3 -3.1 N.D. -4.4 +3.3
stress Vancomycin 0.24 mg/ml -0.3 -1.0 N.D. -1.0 -0.0
stress deoxycholate 0.0125 M -0.2 -0.9 N.D. -0.6 -0.5
nitrogen source nitrogen source nitrate 5mM -0.2 -1.5 N.D. +0.4 -0.1
stress Bile salts 0.0125 M +0.2 +0.6 N.D. -0.8 +0.3
carbon source Ying_OrganicAcid7 rep A; time point 3 -0.3 -0.4 N.D. +1.0 +0.0
stress RCH2 defined with Cobalt chloride 0.04 mM +0.4 -0.5 N.D. +0.6 +0.2
nitrogen source nitrogen source nitrate 5mM -0.5 +0.2 N.D. +0.7 +0.3
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B +0.1 +0.5 N.D. +0.8 -0.7
carbon source Ying_OrganicAcid7 rep B; time point 3 -0.3 -0.1 N.D. +1.2 +0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B -0.2 +0.7 N.D. +0.8 -0.5
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C -0.3 +0.6 N.D. +1.0 -0.4
carbon source 4-Hydroxybenzoic Acid (C) -0.1 +0.5 N.D. +0.8 -0.2
carbon source Ying_Nucleobase14 rep C; time point 2 -0.4 +0.7 N.D. +0.7 +0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -0.1 +0.7 N.D. +0.8 -0.3
nitrogen source nitrogen source nitrite 1mM -0.1 +0.5 N.D. +0.8 -0.1
carbon source Ying_Others16 rep C; time point 1 -0.2 +0.4 N.D. +0.9 +0.1
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -0.1 +0.7 N.D. +0.9 -0.2
stress Lomefloxacin 0.0002 mM +0.1 +0.8 N.D. +0.7 -0.1
carbon source 4-Hydroxybenzoic Acid (C) -0.1 +0.5 N.D. +0.9 +0.2
carbon source propionate (C) -0.1 +0.4 N.D. +1.2 +0.1
carbon source Ying_sugar7 rep C; time point 1 +0.2 +0.7 N.D. +0.9 -0.1
carbon source Ying_AminoAcid20 rep C; time point 3 -0.3 +0.1 N.D. +1.8 +0.1
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +0.1 +1.0 N.D. +0.8 -0.1
nitrogen source Cytidine (N) -0.1 +0.6 N.D. +1.3 +0.0
carbon source Ying_AminoAcid20 rep A; time point 3 +0.1 +0.3 N.D. +1.5 +0.1
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