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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_5581 and Pf6N2E2_5582 are separated by 89 nucleotides
Pf6N2E2_5582 and Pf6N2E2_5583 are separated by 167 nucleotides
Pf6N2E2_5583 and Pf6N2E2_5584 are separated by 198 nucleotides
Pf6N2E2_5584 and Pf6N2E2_5585 are separated by 48 nucleotides
Pf6N2E2_5581 - Formate dehydrogenase-O, major subunit (EC 1.2.1.2), at 6,399,757 to 6,401,865
_5581
Pf6N2E2_5582 - Glutaredoxin-related protein, at 6,401,955 to 6,402,296
_5582
Pf6N2E2_5583 - Bacterioferritin, at 6,402,464 to 6,402,937
_5583
Pf6N2E2_5584 - Bacterioferritin-associated ferredoxin, at 6,403,136 to 6,403,354
_5584
Pf6N2E2_5585 - hypothetical protein, at 6,403,403 to 6,403,552
_5585
Group
Condition
Pf6N2E2
_5581
Pf6N2E2
_5582
Pf6N2E2
_5583
Pf6N2E2
_5584
Pf6N2E2
_5585
stress
molybdate 0.25 M
-0.1
N.D.
-0.5
-2.1
N.D.
motility
inner cut, LB soft agar motility assay
+0.2
N.D.
-0.3
-2.4
N.D.
nitrogen source
L-Serine (N)
-0.1
N.D.
-2.5
+0.3
N.D.
carbon source
octanoate (C)
+0.1
N.D.
+0.0
-2.4
N.D.
motility
outer cut, LB soft agar motility assay
+0.1
N.D.
-0.9
-1.3
N.D.
stress
molybdate 0.25 M
-0.0
N.D.
-0.7
-1.1
N.D.
stress
Chloride 0.5 M
-0.1
N.D.
-0.7
-1.0
N.D.
motility
inner cut, LB soft agar motility assay
+0.1
N.D.
-0.4
-1.5
N.D.
stress
Chloride 0.5 M
-0.1
N.D.
-0.4
-1.3
N.D.
carbon source
D-Lactate (C)
+0.0
N.D.
-0.1
-1.5
N.D.
nitrogen source
nitrogen source nitrite 5mM
+0.0
N.D.
-0.2
-1.2
N.D.
nitrogen source
L-Serine (N)
+0.1
N.D.
-0.2
-1.2
N.D.
carbon source
a-Ketoglutaric (C)
+0.1
N.D.
+0.1
-1.3
N.D.
stress
deoxycholate 0.0125 M
+0.2
N.D.
-0.4
-0.8
N.D.
nitrogen source
nitrogen source Ammonium chloride 20mM
+0.0
N.D.
+0.4
-1.0
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
+0.1
N.D.
+0.4
-1.0
N.D.
stress
Phosphomycin 0.004 mg/ml
+0.0
N.D.
+1.0
-1.4
N.D.
stress
Cobalt chloride 0.6 mM
+0.0
N.D.
+0.7
-1.1
N.D.
nitrogen source
Glucuronamide (N)
-0.2
N.D.
-0.5
+0.8
N.D.
stress
Cobalt chloride 0.6 mM
+0.1
N.D.
+0.9
-0.9
N.D.
stress
Cobalt chloride 0.6 mM
+0.1
N.D.
+1.1
-1.0
N.D.
stress
Bile salts 0.0125 M
+0.1
N.D.
-0.8
+1.3
N.D.
vitamin
With vitamins t1
+0.1
N.D.
-0.2
+1.2
N.D.
stress
Cobalt chloride 0.6 mM
-0.1
N.D.
+1.1
+0.2
N.D.
stress
RCH2 defined with Thallium(I) acetate 0.002 mg/ml
-0.1
N.D.
-0.1
+1.5
N.D.
resistance
10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine
-0.0
N.D.
+0.1
+1.3
N.D.
stress
RCH2 defined with Cobalt chloride 0.04 mM
+0.0
N.D.
+1.2
+0.2
N.D.
stress
Zinc chloride 0.0000015625 M
+0.3
N.D.
+0.5
+0.7
N.D.
carbon source
Ying_sugar7 rep B; time point 2
+0.0
N.D.
+0.3
+1.6
N.D.
stress
RCH2 defined with Cobalt chloride 0.04 mM
-0.0
N.D.
+1.5
+0.5
N.D.
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