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Gene
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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_556 and Pf6N2E2_557 are separated by 24 nucleotides
Pf6N2E2_557 and Pf6N2E2_558 are separated by 63 nucleotides
Pf6N2E2_558 and Pf6N2E2_559 are separated by 18 nucleotides
Pf6N2E2_559 and Pf6N2E2_560 overlap by 4 nucleotides
Pf6N2E2_556 - hypothetical protein, at 627,959 to 628,075
_556
Pf6N2E2_557 - Uncharacterized protein homologous to Paeru. PA2364, at 628,100 to 628,549
_557
Pf6N2E2_558 - Uncharacterized protein ImpJ/VasE, at 628,613 to 629,944
_558
Pf6N2E2_559 - Inner membrane protein DotU, at 629,963 to 630,727
_559
Pf6N2E2_560 - IcmF-related protein, at 630,724 to 634,548
_560
Group
Condition
Pf6N2E2
_556
Pf6N2E2
_557
Pf6N2E2
_558
Pf6N2E2
_559
Pf6N2E2
_560
stress
Cobalt chloride 0.6 mM
N.D.
-0.8
-1.0
+0.2
-0.3
stress
Cobalt chloride 0.6 mM
N.D.
-0.5
-0.7
-0.4
-0.1
stress
Cobalt chloride 0.6 mM
N.D.
-0.3
-0.7
-0.2
-0.4
stress
Nickel (II) chloride 0.0025 M
N.D.
-0.4
-0.1
-0.8
-0.0
stress
Cobalt chloride 0.16 mM
N.D.
-0.2
-0.7
-0.2
-0.3
lb
LB no stress 24 hours
N.D.
-0.5
-0.4
-0.2
-0.1
stress
Vanillyl Alcohol 0.005 M
N.D.
-0.4
-0.4
-0.3
-0.2
stress
deoxycholate 0.0125 M
N.D.
-0.2
-0.0
-0.9
-0.1
stress
Cobalt chloride 0.4 mM
N.D.
-0.7
-0.3
+0.1
-0.3
stress
DEANONOate 0.000625 M
N.D.
-0.2
-0.4
-0.6
+0.0
stress
Cobalt chloride 0.4 mM
N.D.
-0.4
-0.8
+0.0
+0.0
stress
Cobalt chloride 0.6 mM
N.D.
-0.6
-0.5
+0.1
-0.1
carbon source
Ying_sugar7 rep C; time point 2
N.D.
-0.4
-0.2
-0.4
-0.0
vitamin
With vitamins t2
N.D.
+0.1
-0.7
-0.2
-0.1
carbon source
Ying_Others16 rep B; time point 2
N.D.
-0.7
-0.3
-0.1
+0.1
stress
Chloramphenicol 0.008 mg/ml
N.D.
-0.4
-0.3
-0.3
+0.1
motility
inner cut, LB soft agar motility assay
N.D.
-0.4
-0.4
-0.0
+0.2
lb
LB no stress 48 hours
N.D.
+0.2
-0.4
-0.4
+0.0
stress
RCH2 defined with Cobalt chloride 0.02 mM
N.D.
-0.4
+0.1
-0.4
+0.2
nitrogen source
Glucuronamide (N)
N.D.
-0.1
-0.4
+0.3
-0.3
carbon source
Carbon source Sucrose 5 mM
N.D.
-0.5
-0.4
+0.1
+0.3
stress
Manganese (II) chloride tetrahydrate 0.00078125 M
N.D.
-0.3
-0.4
+0.2
+0.1
stress
thiosulfate 0.25 M
N.D.
-0.6
+0.5
-0.1
-0.0
nitrogen source
L-Glutamic (N)
N.D.
-0.3
-0.1
+0.5
-0.2
nitrogen source
L-Serine (N)
N.D.
+0.2
-0.6
+0.3
-0.0
stress
Nickel (II) chloride 1 mM
N.D.
+0.1
-0.6
+0.1
+0.3
stress
Nickel (II) chloride 1.5 mM
N.D.
-0.5
-0.0
+0.4
+0.2
carbon source
octanoate (C)
N.D.
+0.3
+0.2
-0.5
+0.1
stress
RCH2 defined with Nickel (II) chloride 0.04 mM
N.D.
-0.5
+0.3
+0.2
+0.1
stress
sodium iodate 0.005 M
N.D.
+0.2
+0.5
-0.2
+0.3
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