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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_5327 and Pf6N2E2_5328 are separated by 292 nucleotides
Pf6N2E2_5328 and Pf6N2E2_5329 are separated by 141 nucleotides
Pf6N2E2_5329 and Pf6N2E2_5330 are separated by 24 nucleotides
Pf6N2E2_5330 and Pf6N2E2_5331 are separated by 101 nucleotides
Pf6N2E2_5327 - Outer membrane protein assembly factor YaeT precursor, at 6,122,341 to 6,123,078
_5327
Pf6N2E2_5328 - FIG00958649: hypothetical protein, at 6,123,371 to 6,123,805
_5328
Pf6N2E2_5329 - Alpha/beta hydrolase, at 6,123,947 to 6,124,582
_5329
Pf6N2E2_5330 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 6,124,607 to 6,125,962
_5330
Pf6N2E2_5331 - ATPase, AFG1 family, at 6,126,064 to 6,127,158
_5331
Group
Condition
Pf6N2E2
_5327
Pf6N2E2
_5328
Pf6N2E2
_5329
Pf6N2E2
_5330
Pf6N2E2
_5331
nitrogen source
L-Isoleucine (N)
-0.5
-3.3
+0.4
N.D.
-2.4
carbon source
octanoate (C)
-0.1
-5.6
+0.4
N.D.
-0.2
stress
Magnesium chloride 0.125 M
-1.2
-2.2
-0.3
N.D.
-0.5
stress
Hydroxylamine 0.5 M
+0.9
-2.1
+0.0
N.D.
-2.8
motility
outer cut, LB soft agar motility assay
-0.3
-0.2
-0.1
N.D.
-3.3
nitrogen source
L-Isoleucine (N)
-0.2
-2.6
+1.0
N.D.
-1.7
nitrogen source
nitrogen source nitrate 5mM
-0.4
-0.9
-0.8
N.D.
-1.3
stress
Zinc chloride 0.0000015625 M
+0.1
-4.2
+0.6
N.D.
+0.1
stress
RCH2 defined with copper (II) chloride 0.03 mM
-0.0
-3.2
+0.2
N.D.
-0.3
carbon source
Trisodium citrate (C)
-0.3
+0.2
-0.7
N.D.
-2.4
stress
thiosulfate 0.25 M
+0.1
-2.8
+0.1
N.D.
-0.4
stress
DEANONOate 0.000625 M
+0.3
-0.3
-0.0
N.D.
-3.0
carbon source
octanoate (C)
-0.2
-4.0
+0.9
N.D.
+0.4
stress
5-Hydroxymethylfurfural 10 mM
+0.4
-0.5
+0.2
N.D.
-2.9
stress
L-Lysine 25 mM
-0.1
-3.0
+0.1
N.D.
+0.3
stress
Cobalt chloride 0.6 mM
-0.1
-2.0
+0.9
N.D.
-1.3
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
+0.1
-2.7
+0.5
N.D.
+0.1
carbon source
Ying_Nucleobase14 rep C; time point 3
-0.2
-2.5
+0.6
N.D.
+0.4
motility
inner cut, LB soft agar motility assay
+0.3
+0.3
+0.5
N.D.
-2.6
stress
copper (II) chloride 3 mM
+0.2
-2.9
+0.1
N.D.
+1.4
carbon source
L-Alanine (C)
-0.3
-1.9
+0.8
N.D.
+0.4
carbon source
D-Alanine (C)
-0.2
+0.5
+0.8
N.D.
-2.0
stress
Choline Lysine 20 mM
+0.2
-2.6
+0.3
N.D.
+1.5
stress
RCH2 defined with Cobalt chloride 0.04 mM
-0.1
+1.7
-0.1
N.D.
-1.9
stress
periodate 0.00125 M
+0.2
-2.0
+0.1
N.D.
+1.6
carbon source
D-Fructose (C)
-0.3
-2.0
+0.7
N.D.
+2.2
carbon source
m-Inositol (C)
-0.3
-1.2
+0.3
N.D.
+2.1
carbon source
D-Galactose (C)
+0.2
-2.5
+1.8
N.D.
+3.4
carbon source
D-Galactose (C)
-0.0
-1.5
+1.5
N.D.
+2.9
stress
Rifampicin 0.003 mg/ml
-0.3
+1.4
+0.1
N.D.
+1.8
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