Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_5233 and Pf6N2E2_5234 are separated by 119 nucleotidesPf6N2E2_5234 and Pf6N2E2_5235 are separated by 2 nucleotidesPf6N2E2_5235 and Pf6N2E2_5236 are separated by 62 nucleotidesPf6N2E2_5236 and Pf6N2E2_5237 overlap by 21 nucleotides Pf6N2E2_5233 - Ferrochelatase, protoheme ferro-lyase (EC 4.99.1.1), at 6,030,944 to 6,031,969 _5233 Pf6N2E2_5234 - Uracil permease, at 6,032,089 to 6,033,363 _5234 Pf6N2E2_5235 - Uracil phosphoribosyltransferase (EC 2.4.2.9), at 6,033,366 to 6,034,004 _5235 Pf6N2E2_5236 - hypothetical protein, at 6,034,067 to 6,034,189 _5236 Pf6N2E2_5237 - Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8), at 6,034,169 to 6,034,726 _5237
Group Condition Pf6N2E2_5233 Pf6N2E2_5234 Pf6N2E2_5235 Pf6N2E2_5236 Pf6N2E2_5237
stress Nickel (II) chloride 0.0025 M N.D. -0.3 -0.4 -0.7 -4.7
stress thiosulfate 0.25 M N.D. -0.2 -0.1 -2.2 -1.9
stress Nickel (II) chloride 0.0025 M N.D. +0.4 -0.3 +0.1 -4.3
stress Bile salts 0.0125 M N.D. +0.2 -0.4 +0.5 -2.5
nitrogen source nitrogen source nitrite 1mM N.D. +0.1 +0.1 -0.6 -1.6
stress RCH2 defined with copper (II) chloride 0.03 mM N.D. -0.0 +0.4 -1.8 -0.4
nitrogen source nitrogen source nitrate 20mM N.D. +0.6 +0.1 -1.3 -0.9
stress Hydroxylamine 0.5 M N.D. +0.1 +0.5 -0.4 -1.7
motility inner cut, LB soft agar motility assay N.D. +0.0 +0.7 -1.0 -1.1
carbon source Ying_sugar7 rep B; time point 2 N.D. -0.0 -0.1 +0.6 -1.7
carbon source Ying_Nucleobase14 rep B; time point 2 N.D. -0.7 -0.9 +0.5 +0.3
stress phosphite 0.25 M N.D. -0.2 -0.2 +0.8 -1.3
carbon source RCH2_defined N.D. +0.5 +0.2 -1.5 +0.3
nitrogen source nitrogen source nitrite 5mM N.D. +0.5 +0.4 -1.3 +0.3
carbon source Ying_Nucleobase14 rep C; time point 3 N.D. -0.3 -1.1 +0.9 +0.8
stress Fusidic 0.4 mg/ml N.D. -0.3 -0.6 +0.7 +0.8
stress Cobalt chloride 0.6 mM N.D. -0.3 -0.7 +0.4 +1.2
stress Phosphomycin 0.004 mg/ml N.D. -0.6 -1.6 +0.7 +2.1
stress Cobalt chloride 0.4 mM N.D. -0.2 -0.7 +0.6 +1.0
stress Fusidic 0.3 mg/ml N.D. -0.0 -0.7 +0.9 +0.9
carbon source Ying_OrganicAcid7 rep C; time point 3 N.D. -0.1 -0.6 +0.4 +1.4
carbon source Ying_Nucleobase14 rep A; time point 3 N.D. +0.2 -1.2 +1.0 +1.2
stress Cobalt chloride 0.6 mM N.D. -0.7 -1.1 +1.1 +1.7
stress tungstate 0.05 M N.D. +0.1 -0.7 +1.0 +0.8
carbon source Ying_Nucleobase14 rep B; time point 3 N.D. +0.3 -1.4 +0.6 +1.7
stress Cobalt chloride 0.6 mM N.D. -0.7 -0.8 +1.7 +1.2
carbon source Ying_OrganicAcid7 rep B; time point 3 N.D. -0.4 -0.1 +0.5 +1.4
stress Cobalt chloride 0.6 mM N.D. -0.6 -1.1 +1.4 +2.0
nitrogen source L-Serine (N) N.D. -0.4 +0.7 +0.1 +1.7
nitrogen source L-Serine (N) N.D. +0.1 +0.7 -0.1 +1.9
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