Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_5219 and Pf6N2E2_5220 are separated by 250 nucleotidesPf6N2E2_5220 and Pf6N2E2_5221 overlap by 4 nucleotidesPf6N2E2_5221 and Pf6N2E2_5222 are separated by 14 nucleotidesPf6N2E2_5222 and Pf6N2E2_5223 overlap by 7 nucleotides Pf6N2E2_5219 - FIG140336: TPR domain protein, at 6,016,736 to 6,018,442 _5219 Pf6N2E2_5220 - Glutamyl-tRNA reductase (EC 1.2.1.70), at 6,018,693 to 6,019,922 _5220 Pf6N2E2_5221 - Peptide chain release factor 1, at 6,019,919 to 6,021,001 _5221 Pf6N2E2_5222 - Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2, at 6,021,016 to 6,021,846 _5222 Pf6N2E2_5223 - Molybdopterin biosynthesis protein MoeB, at 6,021,840 to 6,022,595 _5223
Group Condition Pf6N2E2_5219 Pf6N2E2_5220 Pf6N2E2_5221 Pf6N2E2_5222 Pf6N2E2_5223
nitrogen source nitrogen source nitrate 20mM -1.2 N.D. N.D. N.D. -5.2
nitrogen source nitrogen source nitrate 5mM -1.5 N.D. N.D. N.D. -4.2
carbon source octanoate (C) -3.7 N.D. N.D. N.D. -1.8
carbon source Putrescine (C) -5.5 N.D. N.D. N.D. +0.3
carbon source octanoate (C) -4.2 N.D. N.D. N.D. -0.9
stress Nalidixic 0.004 mg/ml -4.2 N.D. N.D. N.D. -0.5
carbon source Putrescine (C) -5.1 N.D. N.D. N.D. +0.4
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B +1.5 N.D. N.D. N.D. -5.7
vitamin No vitamins t2 -0.4 N.D. N.D. N.D. -3.8
stress Tetracycline 0.0002 mg/ml -3.8 N.D. N.D. N.D. -0.3
stress Chloramphenicol 0.004 mg/ml -4.0 N.D. N.D. N.D. -0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C +0.3 N.D. N.D. N.D. -4.4
nitrogen source nitrogen source nitrate 20mM -1.4 N.D. N.D. N.D. -2.5
stress sodium iodate 0.005 M -3.6 N.D. N.D. N.D. -0.2
stress periodate 0.00125 M -3.0 N.D. N.D. N.D. -0.8
stress Nalidixic 0.008 mg/ml -3.8 N.D. N.D. N.D. +0.0
carbon source Ying_Nucleobase14 rep C; time point 3 -1.2 N.D. N.D. N.D. -2.5
carbon source Ying_Nucleobase14 rep A; time point 3 -1.2 N.D. N.D. N.D. -2.5
nitrogen source L-Serine (N) -2.4 N.D. N.D. N.D. -1.3
carbon source Ying_Nucleobase14 rep B; time point 2 -1.1 N.D. N.D. N.D. -2.6
stress Lomefloxacin 0.0002 mM -3.6 N.D. N.D. N.D. +0.2
denitrifying Nitrate reduction, replicate B +1.0 N.D. N.D. N.D. -4.3
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B +1.2 N.D. N.D. N.D. -4.5
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A +1.1 N.D. N.D. N.D. -4.0
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A +1.4 N.D. N.D. N.D. -4.3
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C +1.3 N.D. N.D. N.D. -3.8
denitrifying Nitrate reduction, replicate C +0.9 N.D. N.D. N.D. -3.4
denitrifying Nitrate reduction, replicate A +1.9 N.D. N.D. N.D. -3.1
motility inner cut, LB soft agar motility assay +1.6 N.D. N.D. N.D. -2.5
stress Vancomycin 0.24 mg/ml +5.3 N.D. N.D. N.D. +0.3
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