Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_5120 and Pf6N2E2_5121 are separated by 621 nucleotidesPf6N2E2_5121 and Pf6N2E2_5122 are separated by 224 nucleotidesPf6N2E2_5122 and Pf6N2E2_5123 overlap by 4 nucleotidesPf6N2E2_5123 and Pf6N2E2_5124 overlap by 11 nucleotides Pf6N2E2_5120 - Glycine oxidase ThiO (EC 1.4.3.19), at 5,903,287 to 5,904,387 _5120 Pf6N2E2_5121 - hypothetical protein, at 5,905,009 to 5,905,209 _5121 Pf6N2E2_5122 - Type IV fimbriae expression regulatory protein PilR, at 5,905,434 to 5,906,780 _5122 Pf6N2E2_5123 - Two-component sensor PilS, at 5,906,777 to 5,908,366 _5123 Pf6N2E2_5124 - FIG00953647: hypothetical protein, at 5,908,356 to 5,908,589 _5124
Group Condition Pf6N2E2_5120 Pf6N2E2_5121 Pf6N2E2_5122 Pf6N2E2_5123 Pf6N2E2_5124
stress copper (II) chloride 3 mM -0.2 N.D. +0.4 +0.0 -1.9
nitrogen source Gly-Glu (N) -0.2 N.D. -0.3 -0.2 -0.7
carbon source L-Leucine (C) -0.1 N.D. -0.8 -0.1 -0.0
carbon source Trisodium citrate (C) +0.0 N.D. -0.6 -0.1 -0.2
motility inner cut, LB soft agar motility assay -0.1 N.D. -0.7 -0.0 -0.1
stress nitrate 0.125 M -0.3 N.D. -0.0 +0.4 -0.7
stress Fusidic 0.3 mg/ml -0.2 N.D. +0.2 -0.3 -0.3
nitrogen source Parabanic Acid (N) +0.2 N.D. +0.1 -0.1 -0.6
carbon source L-Leucine (C) +0.0 N.D. -0.7 +0.0 +0.3
carbon source Ying_AminoAcid20 rep B; time point 2 +0.3 N.D. -0.5 -0.3 +0.1
nitrogen source nitrogen source nitrate 20mM +0.3 N.D. -0.1 +0.2 -0.6
stress RCH2 defined with Cobalt chloride 0.04 mM +0.0 N.D. -0.3 +0.3 -0.3
nitrogen source Glucuronamide (N) +0.2 N.D. +0.2 -0.1 -0.5
motility inner cut, LB soft agar motility assay +0.2 N.D. -0.1 +0.3 -0.5
nitrogen source nitrogen source nitrite 1mM +0.2 N.D. -0.5 +0.2 +0.0
stress RCH2 defined with Nickel (II) chloride 0.04 mM +0.0 N.D. +0.2 +0.2 -0.5
stress sodium carbonate 0.0125 M +0.1 N.D. +0.4 -0.1 -0.4
carbon source Ying_Nucleobase14 rep C; time point 1 +0.4 N.D. -0.4 -0.1 +0.2
carbon source Ying_Nucleobase14 rep C; time point 2 +0.4 N.D. +0.2 -0.3 +0.1
carbon source Carbon source D-Glucose 10 mM +0.1 N.D. +0.5 -0.2 -0.1
stress thiosulfate 0.25 M +0.2 N.D. +0.4 +0.1 -0.2
nitrogen source L-Serine (N) +0.8 N.D. +0.1 +0.1 -0.6
denitrifying Nitrate reduction, replicate A +0.1 N.D. +0.3 +0.3 -0.2
stress Chlorite 0.00005 M +0.2 N.D. +0.3 +0.2 -0.2
stress DEANONOate 0.000625 M +0.3 N.D. +0.5 -0.1 -0.3
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C +0.0 N.D. +0.4 +0.3 -0.1
stress sodium iodate 0.005 M +0.1 N.D. -0.2 +0.2 +0.6
nitrogen source nitrogen source nitrate 5mM +0.4 N.D. +0.3 +0.3 +0.1
stress Zinc chloride 0.0000015625 M +0.1 N.D. +0.3 +0.1 +0.5
lb LB no stress 24 hours +0.3 N.D. +0.3 +0.3 +0.3
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