Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_506 and Pf6N2E2_507 overlap by 4 nucleotidesPf6N2E2_507 and Pf6N2E2_508 overlap by 8 nucleotidesPf6N2E2_508 and Pf6N2E2_509 are separated by 281 nucleotidesPf6N2E2_509 and Pf6N2E2_510 are separated by 30 nucleotides Pf6N2E2_506 - Hydrogen cyanide synthase HcnA / Opine oxidase subunit C, at 575,019 to 575,342 _506 Pf6N2E2_507 - Hydrogen cyanide synthase HcnB / Opine oxidase subunit A, at 575,339 to 576,748 _507 Pf6N2E2_508 - Hydrogen cyanide synthase HcnC / Opine oxidase subunit B, at 576,741 to 578,003 _508 Pf6N2E2_509 - Glutathione S-transferase, unnamed subgroup 2 (EC 2.5.1.18), at 578,285 to 578,890 _509 Pf6N2E2_510 - Predicted transcriptional regulator of the myo-inositol catabolic operon, at 578,921 to 579,844 _510
Group Condition Pf6N2E2_506 Pf6N2E2_507 Pf6N2E2_508 Pf6N2E2_509 Pf6N2E2_510
carbon source D-Alanine (C) -0.4 +0.1 -0.3 -0.2 -1.7
carbon source octanoate (C) -0.6 -0.1 -0.5 -0.6 -0.5
stress Chloride 600 mM -1.2 -0.8 +0.2 +0.0 -0.3
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine -0.2 -0.3 -0.0 -0.2 -1.4
carbon source L-Phenylalanine (C) -0.0 +0.2 -0.3 -0.7 -1.2
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -0.2 -0.2 -0.3 +0.1 -1.4
carbon source D-Glucosamine Hydrochloride (C) +0.4 +0.0 -0.1 -0.0 -2.2
carbon source Putrescine (C) -0.1 +0.3 -0.1 -0.2 -1.7
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -0.5 -0.1 +0.0 +0.3 -1.5
carbon source Putrescine (C) -0.3 +0.2 +0.2 -0.1 -1.6
carbon source D-Alanine (C) +0.3 +0.5 -0.3 -0.0 -1.9
carbon source L-Leucine (C) +0.2 +0.4 -0.1 -0.2 -1.4
carbon source D-Glucosamine Hydrochloride (C) +0.5 +0.3 +0.3 -0.3 -1.8
denitrifying Nitrate reduction, replicate A +0.3 +0.1 -0.1 +0.3 -1.3
motility inner cut, LB soft agar motility assay -0.6 -0.4 -0.3 -0.3 +1.0
motility outer cut, LB soft agar motility assay -0.4 -0.3 -0.3 -0.3 +0.9
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C -0.3 +0.3 +0.4 +0.4 -1.1
stress RCH2 defined with Cobalt chloride 0.04 mM +1.0 -0.6 -0.2 +0.3 -0.8
stress Hydroxylamine 0.5 M -0.5 +0.4 -0.8 -0.1 +0.8
stress Bile salts 0.0125 M -0.5 +0.2 +0.3 -0.5 +0.7
lb LB no stress 48 hours -0.8 +0.5 +0.3 +0.2 +0.6
carbon source Ying_Others16 rep C; time point 3 +0.1 +0.5 +0.4 +0.6 -0.5
carbon source Ying_sugar7 rep B; time point 2 -0.4 +0.6 +0.4 +0.7 +0.4
motility inner cut, LB soft agar motility assay +0.5 +0.4 -0.2 +0.4 +1.0
carbon source D-Xylose (C) +0.2 +0.1 -0.5 +0.2 +2.5
carbon source m-Inositol (C) +0.2 +0.4 +0.1 +0.2 +2.1
carbon source D-Xylose (C) +0.2 +0.3 -0.2 +0.3 +2.4
carbon source D-Galactose (C) +0.6 +0.2 -0.1 +0.0 +3.7
carbon source D-Galactose (C) +0.6 +0.2 -0.0 -0.0 +4.1
carbon source D-Fructose (C) +0.2 +0.5 +0.0 +0.2 +4.6
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