Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_5058 and Pf6N2E2_5059 are separated by 103 nucleotidesPf6N2E2_5059 and Pf6N2E2_5060 are separated by 57 nucleotidesPf6N2E2_5060 and Pf6N2E2_5061 are separated by 319 nucleotidesPf6N2E2_5061 and Pf6N2E2_5062 overlap by 4 nucleotides Pf6N2E2_5058 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 5,832,424 to 5,834,325 _5058 Pf6N2E2_5059 - Transcriptional regulator, TetR family, at 5,834,429 to 5,835,106 _5059 Pf6N2E2_5060 - hypothetical protein, at 5,835,164 to 5,836,111 _5060 Pf6N2E2_5061 - Quinate/shikimate 5-dehydrogenase I delta (EC 1.1.1.25), at 5,836,431 to 5,837,288 _5061 Pf6N2E2_5062 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 5,837,285 to 5,837,728 _5062
Group Condition Pf6N2E2_5058 Pf6N2E2_5059 Pf6N2E2_5060 Pf6N2E2_5061 Pf6N2E2_5062
stress Fusidic 0.4 mg/ml -0.2 -1.1 N.D. -0.3 -0.0
nitrogen source L-Lysine (N) +0.1 +0.3 N.D. -1.4 -0.2
stress Cobalt chloride 0.6 mM -0.2 -0.9 N.D. +0.3 -0.4
stress RCH2 defined with Cobalt chloride 0.04 mM -0.1 -0.4 N.D. -0.8 +0.5
carbon source D-Trehalose (C) -0.0 +0.9 N.D. -0.7 -0.6
stress Cobalt chloride 0.6 mM -0.1 -0.8 N.D. +0.8 -0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C -0.0 +0.6 N.D. -0.5 +0.5
motility outer cut, LB soft agar motility assay +0.4 +1.3 N.D. -0.2 -0.6
carbon source D-Trehalose (C) +0.1 +0.9 N.D. +0.3 -0.4
motility outer cut, LB soft agar motility assay +0.2 +1.0 N.D. -0.3 +0.1
carbon source D-Galactose (C) +0.2 +0.8 N.D. +0.4 -0.3
motility inner cut, LB soft agar motility assay +0.3 +1.0 N.D. -0.2 +0.2
carbon source L-Phenylalanine (C) +0.3 +0.8 N.D. +0.5 -0.3
carbon source D-Galactose (C) +0.2 +0.8 N.D. -0.2 +0.6
motility inner cut, LB soft agar motility assay +0.4 +1.1 N.D. -0.1 +0.1
nitrogen source L-Serine (N) -0.2 +0.6 N.D. +1.0 +0.2
nitrogen source Cytidine (N) +0.3 +0.9 N.D. -0.2 +0.7
motility inner cut, LB soft agar motility assay +0.3 +0.7 N.D. +0.3 +1.0
stress periodate 0.00125 M +0.6 +1.8 N.D. +0.2 +0.3
carbon source Ying_all64 rep C; time point 3 +0.2 +6.1 N.D. +0.2 -0.0
carbon source Ying_Others16 rep C; time point 1 +0.0 +6.4 N.D. -0.1 +0.2
carbon source Ying_all64 rep A; time point 3 +0.1 +6.3 N.D. +0.2 -0.0
carbon source Ying_all64 rep B; time point 3 +0.1 +6.2 N.D. +0.1 +0.3
carbon source Ying_Others16 rep A; time point 1 +0.2 +6.3 N.D. +0.1 +0.2
carbon source Ying_Others16 rep A; time point 2 +0.1 +7.5 N.D. -0.2 -0.0
carbon source Ying_Others16 rep B; time point 2 +0.0 +7.5 N.D. -0.2 +0.3
carbon source Ying_Others16 rep A; time point 3 -0.1 +8.4 N.D. -0.4 -0.2
carbon source Ying_Others16 rep C; time point 2 +0.0 +7.7 N.D. +0.0 +0.1
carbon source Ying_Others16 rep C; time point 3 -0.0 +8.5 N.D. -0.2 -0.3
carbon source Ying_Others16 rep B; time point 3 -0.0 +8.4 N.D. +0.1 -0.2
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