Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_5043 and Pf6N2E2_5044 are separated by 169 nucleotidesPf6N2E2_5044 and Pf6N2E2_5045 are separated by 161 nucleotidesPf6N2E2_5045 and Pf6N2E2_5046 are separated by 58 nucleotidesPf6N2E2_5046 and Pf6N2E2_5047 are separated by 66 nucleotides Pf6N2E2_5043 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 5,819,299 to 5,819,910 _5043 Pf6N2E2_5044 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 5,820,080 to 5,820,532 _5044 Pf6N2E2_5045 - Permease of the drug/metabolite transporter (DMT) superfamily, at 5,820,694 to 5,821,596 _5045 Pf6N2E2_5046 - ATP-dependent RNA helicase PA3950, at 5,821,655 to 5,822,992 _5046 Pf6N2E2_5047 - NAD(FAD)-utilizing dehydrogenases, at 5,823,059 to 5,824,351 _5047
Group Condition Pf6N2E2_5043 Pf6N2E2_5044 Pf6N2E2_5045 Pf6N2E2_5046 Pf6N2E2_5047
nitrogen source L-Phenylalanine (N) N.D. -1.0 -1.4 -0.1 -0.2
nitrogen source L-Isoleucine (N) N.D. -0.6 -1.8 +0.0 -0.1
nitrogen source L-Phenylalanine (N) N.D. -0.6 -1.3 -0.2 -0.3
nitrogen source L-Isoleucine (N) N.D. +0.6 -2.1 -0.2 +0.0
stress Fluorophosphate 0.125 M N.D. -0.7 -0.1 -0.2 -0.6
stress rubidium chloride 0.25 M N.D. -0.5 -0.1 -0.5 -0.3
stress RCH2 defined with Cobalt chloride 0.02 mM N.D. -0.7 -0.1 -0.3 -0.4
stress sodium iodate 0.005 M N.D. -0.8 -0.2 +0.0 -0.4
nitrogen source nitrogen source nitrite 1mM N.D. -0.9 +0.2 -0.2 -0.1
nitrogen source L-Citrulline (N) N.D. -0.5 +0.2 -0.4 -0.3
stress RCH2 defined with Cobalt chloride 0.04 mM N.D. -1.0 +0.0 -0.5 +0.5
vitamin No vitamins t2 N.D. -0.8 -0.1 +0.3 -0.2
stress selenate 0.5 M N.D. -0.6 -0.2 +0.3 -0.3
stress RCH2 defined with Cobalt chloride 0.04 mM N.D. +0.3 -0.3 -0.7 -0.1
carbon source RCH2_defined N.D. +0.4 -0.2 -0.6 -0.4
nitrogen source nitrogen source nitrate 20mM N.D. -0.4 -0.1 -0.6 +0.5
motility inner cut, LB soft agar motility assay N.D. +0.5 +0.1 -0.6 -0.3
stress Vancomycin 0.24 mg/ml N.D. -0.8 -0.1 +0.6 -0.0
stress Cobalt chloride 0.6 mM N.D. -0.4 -0.1 -0.3 +0.7
stress Chloride 0.5 M N.D. +0.4 -0.2 +0.3 -0.7
stress Fusidic 0.4 mg/ml N.D. -0.6 -0.1 +1.0 -0.2
stress RCH2 defined with Cobalt chloride 0.02 mM N.D. +1.0 -0.3 -0.4 +0.1
stress Hydroxylamine 0.5 M N.D. +0.7 -0.5 +0.4 -0.1
stress Rifampicin 0.003 mg/ml N.D. -0.3 -0.1 +0.8 +0.5
lb LB no stress 48 hours N.D. +0.9 -0.2 -0.0 +0.3
carbon source octanoate (C) N.D. +0.9 +0.0 +0.4 -0.3
stress Tetracycline 0.0002 mg/ml N.D. +0.3 -0.0 +1.0 -0.2
stress Nalidixic 0.008 mg/ml N.D. +0.6 -0.0 +0.3 +0.4
nitrogen source Gly-Glu (N) N.D. +0.8 +0.1 +0.3 +0.3
stress Lomefloxacin 0.0002 mM N.D. +0.4 -0.0 +3.7 +0.1
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