Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_4918 and Pf6N2E2_4919 overlap by 4 nucleotidesPf6N2E2_4919 and Pf6N2E2_4920 are separated by 46 nucleotidesPf6N2E2_4920 and Pf6N2E2_4921 are separated by 16 nucleotidesPf6N2E2_4921 and Pf6N2E2_4922 are separated by 301 nucleotides Pf6N2E2_4918 - Ribonucleotide reductase transcriptional regulator NrdR, at 5,672,761 to 5,673,327 _4918 Pf6N2E2_4919 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 5,673,324 to 5,674,457 _4919 Pf6N2E2_4920 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 5,674,504 to 5,675,169 _4920 Pf6N2E2_4921 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 5,675,186 to 5,676,277 _4921 Pf6N2E2_4922 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 5,676,579 to 5,677,055 _4922
Group Condition Pf6N2E2_4918 Pf6N2E2_4919 Pf6N2E2_4920 Pf6N2E2_4921 Pf6N2E2_4922
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A -1.3 N.D. N.D. -3.5 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C -0.5 N.D. N.D. -3.8 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B -1.2 N.D. N.D. -2.9 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B -0.3 N.D. N.D. -3.4 N.D.
denitrifying Nitrate reduction, replicate B -0.5 N.D. N.D. -2.8 N.D.
nitrogen source nitrogen source nitrate 20mM -3.7 N.D. N.D. +0.6 N.D.
denitrifying Nitrate reduction, replicate A -0.8 N.D. N.D. -1.9 N.D.
vitamin With vitamins t2 -1.7 N.D. N.D. -1.0 N.D.
stress Nickel (II) chloride 0.0025 M -1.6 N.D. N.D. -0.7 N.D.
stress Zinc chloride 0.0000015625 M -2.3 N.D. N.D. +0.1 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A +0.2 N.D. N.D. -2.4 N.D.
vitamin No vitamins t2 -1.0 N.D. N.D. -1.1 N.D.
lb LB no stress 48 hours -1.5 N.D. N.D. -0.7 N.D.
stress Hydroxylamine 0.5 M -1.3 N.D. N.D. -0.8 N.D.
lb LB no stress 24 hours -1.1 N.D. N.D. -1.0 N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C -0.6 N.D. N.D. -1.5 N.D.
stress antimonate 0.005 M -1.3 N.D. N.D. -0.7 N.D.
stress Cobalt chloride 0.4 mM -1.4 N.D. N.D. -0.6 N.D.
stress Chloride 0.5 M -0.7 N.D. N.D. -1.3 N.D.
lb LB no stress 48 hours -1.4 N.D. N.D. -0.5 N.D.
stress Fluorophosphate 0.125 M -0.3 N.D. N.D. -1.5 N.D.
stress 5-Hydroxymethylfurfural 10 mM -1.1 N.D. N.D. -0.8 N.D.
carbon source Ying_sugar7 rep B; time point 1 -1.7 N.D. N.D. -0.2 N.D.
stress Cobalt chloride 0.6 mM -2.0 N.D. N.D. +0.2 N.D.
nitrogen source nitrogen source nitrite 1mM -2.3 N.D. N.D. +0.4 N.D.
vitamin No vitamins t1 -1.1 N.D. N.D. -0.7 N.D.
carbon source Ying_OrganicAcid7 rep A; time point 3 -1.6 N.D. N.D. -0.2 N.D.
stress D-Cycloserine 0.12 mg/ml -1.8 N.D. N.D. +0.2 N.D.
lb LB no stress 48 hours -2.0 N.D. N.D. +0.6 N.D.
nitrogen source L-Serine (N) +0.9 N.D. N.D. +0.9 N.D.
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