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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_4918 and Pf6N2E2_4919 overlap by 4 nucleotides
Pf6N2E2_4919 and Pf6N2E2_4920 are separated by 46 nucleotides
Pf6N2E2_4920 and Pf6N2E2_4921 are separated by 16 nucleotides
Pf6N2E2_4921 and Pf6N2E2_4922 are separated by 301 nucleotides
Pf6N2E2_4918 - Ribonucleotide reductase transcriptional regulator NrdR, at 5,672,761 to 5,673,327
_4918
Pf6N2E2_4919 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 5,673,324 to 5,674,457
_4919
Pf6N2E2_4920 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 5,674,504 to 5,675,169
_4920
Pf6N2E2_4921 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 5,675,186 to 5,676,277
_4921
Pf6N2E2_4922 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 5,676,579 to 5,677,055
_4922
Group
Condition
Pf6N2E2
_4918
Pf6N2E2
_4919
Pf6N2E2
_4920
Pf6N2E2
_4921
Pf6N2E2
_4922
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
-1.3
N.D.
N.D.
-3.5
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
-0.5
N.D.
N.D.
-3.8
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
-1.2
N.D.
N.D.
-2.9
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
-0.3
N.D.
N.D.
-3.4
N.D.
denitrifying
Nitrate reduction, replicate B
-0.5
N.D.
N.D.
-2.8
N.D.
nitrogen source
nitrogen source nitrate 20mM
-3.7
N.D.
N.D.
+0.6
N.D.
denitrifying
Nitrate reduction, replicate A
-0.8
N.D.
N.D.
-1.9
N.D.
vitamin
With vitamins t2
-1.7
N.D.
N.D.
-1.0
N.D.
stress
Nickel (II) chloride 0.0025 M
-1.6
N.D.
N.D.
-0.7
N.D.
stress
Zinc chloride 0.0000015625 M
-2.3
N.D.
N.D.
+0.1
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
+0.2
N.D.
N.D.
-2.4
N.D.
vitamin
No vitamins t2
-1.0
N.D.
N.D.
-1.1
N.D.
lb
LB no stress 48 hours
-1.5
N.D.
N.D.
-0.7
N.D.
stress
Hydroxylamine 0.5 M
-1.3
N.D.
N.D.
-0.8
N.D.
lb
LB no stress 24 hours
-1.1
N.D.
N.D.
-1.0
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
-0.6
N.D.
N.D.
-1.5
N.D.
stress
antimonate 0.005 M
-1.3
N.D.
N.D.
-0.7
N.D.
stress
Cobalt chloride 0.4 mM
-1.4
N.D.
N.D.
-0.6
N.D.
stress
Chloride 0.5 M
-0.7
N.D.
N.D.
-1.3
N.D.
lb
LB no stress 48 hours
-1.4
N.D.
N.D.
-0.5
N.D.
stress
Fluorophosphate 0.125 M
-0.3
N.D.
N.D.
-1.5
N.D.
stress
5-Hydroxymethylfurfural 10 mM
-1.1
N.D.
N.D.
-0.8
N.D.
carbon source
Ying_sugar7 rep B; time point 1
-1.7
N.D.
N.D.
-0.2
N.D.
stress
Cobalt chloride 0.6 mM
-2.0
N.D.
N.D.
+0.2
N.D.
nitrogen source
nitrogen source nitrite 1mM
-2.3
N.D.
N.D.
+0.4
N.D.
vitamin
No vitamins t1
-1.1
N.D.
N.D.
-0.7
N.D.
carbon source
Ying_OrganicAcid7 rep A; time point 3
-1.6
N.D.
N.D.
-0.2
N.D.
stress
D-Cycloserine 0.12 mg/ml
-1.8
N.D.
N.D.
+0.2
N.D.
lb
LB no stress 48 hours
-2.0
N.D.
N.D.
+0.6
N.D.
nitrogen source
L-Serine (N)
+0.9
N.D.
N.D.
+0.9
N.D.
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