Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_4798 and Pf6N2E2_4799 are separated by 63 nucleotidesPf6N2E2_4799 and Pf6N2E2_4800 overlap by 4 nucleotidesPf6N2E2_4800 and Pf6N2E2_4801 are separated by 113 nucleotidesPf6N2E2_4801 and Pf6N2E2_4802 are separated by 300 nucleotides Pf6N2E2_4798 - tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25), at 5,546,436 to 5,547,662 _4798 Pf6N2E2_4799 - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data), at 5,547,726 to 5,549,291 _4799 Pf6N2E2_4800 - yeaH component of nitrogen-related signalling system yeaH (of yeaGH-ycgB) (from data), at 5,549,288 to 5,550,559 _4800 Pf6N2E2_4801 - yeaG component of nitrogen-related signalling system (of yeaGH-ycgB) (from data), at 5,550,673 to 5,552,595 _4801 Pf6N2E2_4802 - Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1), at 5,552,896 to 5,553,225 _4802
Group Condition Pf6N2E2_4798 Pf6N2E2_4799 Pf6N2E2_4800 Pf6N2E2_4801 Pf6N2E2_4802
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. -2.3 -2.6 -2.3 -0.9
carbon source Ying_OrganicAcid7 rep C; time point 3 N.D. -2.8 -2.3 -2.4 -0.4
carbon source Ying_OrganicAcid7 rep B; time point 3 N.D. -2.7 -2.3 -2.3 -0.4
carbon source Ying_OrganicAcid7 rep A; time point 3 N.D. -2.8 -2.0 -2.4 -0.5
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. -2.0 -2.2 -1.9 -0.7
carbon source propionate (C) N.D. -2.1 -2.1 -2.1 -0.1
carbon source propionate (C) N.D. -1.9 -2.5 -1.6 -0.4
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine N.D. -1.8 -1.9 -1.7 -0.6
resistance 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. -1.6 -1.8 -1.6 -0.5
carbon source Ying_OrganicAcid7 rep B; time point 2 N.D. -1.7 -1.3 -1.6 -0.4
resistance 10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine N.D. -1.5 -1.6 -1.3 -0.6
carbon source Ying_OrganicAcid7 rep A; time point 2 N.D. -1.5 -1.4 -1.5 -0.3
stress RCH2 defined with Zinc 0.032 mM N.D. -1.5 -1.5 -1.3 -0.3
carbon source Ying_OrganicAcid7 rep C; time point 2 N.D. -1.6 -1.3 -1.6 -0.0
resistance 20 mM putrescine N.D. -1.3 -1.5 -1.3 -0.2
stress RCH2 defined with Zinc 0.032 mM N.D. -1.2 -1.2 -1.4 -0.4
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. -1.5 -1.4 -1.2 +0.0
carbon source Ying_Nucleobase14 rep A; time point 3 N.D. -0.8 -0.6 -0.9 -0.7
carbon source Ying_Nucleobase14 rep C; time point 3 N.D. -0.8 -0.5 -1.0 -0.2
carbon source D-Sorbitol (C) N.D. -0.7 -0.8 -0.7 -0.4
carbon source Ying_AminoAcid20 rep B; time point 3 N.D. -0.6 -0.4 -0.8 -0.7
carbon source D-Glucose (C) N.D. -0.7 -0.8 -0.6 -0.3
carbon source Ying_Nucleobase14 rep B; time point 3 N.D. -0.8 -0.4 -0.9 -0.2
carbon source Ying_AminoAcid20 rep C; time point 3 N.D. -0.9 -0.6 -0.9 -0.0
carbon source D-Glucose (C) N.D. -0.9 -0.9 -0.5 -0.0
carbon source Ying_AminoAcid20 rep A; time point 3 N.D. -0.7 -0.5 -0.8 -0.3
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A N.D. -0.7 -0.7 -0.6 -0.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C N.D. -0.5 -0.6 -0.7 -0.5
stress Nickel (II) chloride 0.0025 M N.D. +0.6 +0.6 +0.5 +0.8
stress Nickel (II) chloride 0.0025 M N.D. +0.8 +0.8 +0.8 +1.1
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