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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_4530 and Pf6N2E2_4531 are separated by 157 nucleotides
Pf6N2E2_4531 and Pf6N2E2_4532 are separated by 2 nucleotides
Pf6N2E2_4532 and Pf6N2E2_4533 overlap by 14 nucleotides
Pf6N2E2_4533 and Pf6N2E2_4534 are separated by 115 nucleotides
Pf6N2E2_4530 - Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV), at 5,263,855 to 5,265,942
_4530
Pf6N2E2_4531 - hypothetical protein, at 5,266,100 to 5,266,993
_4531
Pf6N2E2_4532 - Flagellum-specific ATP synthase FliI, at 5,266,996 to 5,268,351
_4532
Pf6N2E2_4533 - RspO, at 5,268,338 to 5,268,757
_4533
Pf6N2E2_4534 - hypothetical protein, at 5,268,873 to 5,269,319
_4534
Group
Condition
Pf6N2E2
_4530
Pf6N2E2
_4531
Pf6N2E2
_4532
Pf6N2E2
_4533
Pf6N2E2
_4534
stress
bromate 0.05 M
-0.7
-2.4
+0.3
N.D.
-0.5
nitrogen source
L-Serine (N)
-0.0
-1.6
-0.1
N.D.
-0.7
nitrogen source
nitrogen source nitrite 5mM
-0.3
-1.3
-0.5
N.D.
-0.2
nitrogen source
Glucuronamide (N)
-0.5
-0.6
-0.2
N.D.
-0.9
nitrogen source
nitrogen source Ammonium chloride 20mM
-0.5
-1.4
-0.1
N.D.
+0.1
carbon source
Ying_Others16 rep C; time point 1
+0.2
-0.9
+0.3
N.D.
-1.6
stress
molybdate 0.25 M
+0.1
-1.3
-0.1
N.D.
-0.6
carbon source
Ying_OrganicAcid7 rep B; time point 3
-0.8
+0.2
-0.0
N.D.
-1.2
lb
LB no stress 48 hours
-0.3
-0.9
+0.2
N.D.
-0.6
stress
Hydroxylamine 0.5 M
-0.5
+0.5
+0.0
N.D.
-1.5
carbon source
Ying_OrganicAcid7 rep B; time point 2
+0.1
-0.9
+0.2
N.D.
-0.8
carbon source
Ying_AminoAcid20 rep C; time point 2
+0.2
-1.0
+0.2
N.D.
-0.8
carbon source
Ying_Nucleobase14 rep C; time point 2
+0.3
-0.9
+0.2
N.D.
-1.0
nitrogen source
Cytidine (N)
+0.2
-1.8
-0.0
N.D.
+0.4
carbon source
Ying_AminoAcid20 rep A; time point 2
+0.2
-1.0
+0.4
N.D.
-0.9
carbon source
Ying_AminoAcid20 rep B; time point 2
+0.3
-1.0
+0.5
N.D.
-1.0
lb
LB no stress 48 hours
-0.4
+0.1
-1.2
N.D.
+0.5
stress
Zinc chloride 0.0000015625 M
-0.0
-1.5
-0.5
N.D.
+1.1
carbon source
Ying_Nucleobase14 rep B; time point 1
+0.4
-0.9
+0.5
N.D.
-0.5
nitrogen source
Glucuronamide (N)
-0.3
+1.2
-0.3
N.D.
-1.1
carbon source
Ying_Nucleobase14 rep C; time point 1
+0.5
-0.9
+0.4
N.D.
-0.4
stress
Nickel (II) chloride 1 mM
-0.2
+1.0
+0.1
N.D.
-1.2
stress
Spectinomycin 0.01 mg/ml
-0.1
+1.0
+0.2
N.D.
-0.9
motility
outer cut, LB soft agar motility assay
-0.7
+0.7
+0.2
N.D.
+0.5
stress
Nickel (II) chloride 1 mM
-0.0
+1.4
-0.2
N.D.
-0.5
carbon source
D-Galactose (C)
-0.3
+2.0
-0.3
N.D.
+0.0
stress
RCH2 defined with Zinc 0.032 mM
-0.4
+0.7
+0.0
N.D.
+1.0
carbon source
D-Fructose (C)
+0.3
+2.2
-0.3
N.D.
-0.5
carbon source
m-Inositol (C)
+0.1
+1.8
+0.1
N.D.
-0.0
carbon source
D-Galactose (C)
+0.5
+2.6
+0.1
N.D.
+0.2
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