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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_4414 and Pf6N2E2_4415 are separated by 207 nucleotides
Pf6N2E2_4415 and Pf6N2E2_4416 overlap by 4 nucleotides
Pf6N2E2_4416 and Pf6N2E2_4417 are separated by 2 nucleotides
Pf6N2E2_4417 and Pf6N2E2_4418 are separated by 13 nucleotides
Pf6N2E2_4414 - FIG00953808: hypothetical protein, at 5,148,368 to 5,148,691
_4414
Pf6N2E2_4415 - Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase, at 5,148,899 to 5,149,603
_4415
Pf6N2E2_4416 - Tyrosine recombinase XerC, at 5,149,600 to 5,150,499
_4416
Pf6N2E2_4417 - Protein of unknown function DUF484, at 5,150,502 to 5,151,224
_4417
Pf6N2E2_4418 - Diaminopimelate epimerase (EC 5.1.1.7), at 5,151,238 to 5,152,068
_4418
Group
Condition
Pf6N2E2
_4414
Pf6N2E2
_4415
Pf6N2E2
_4416
Pf6N2E2
_4417
Pf6N2E2
_4418
carbon source
octanoate (C)
-7.0
+0.0
-0.7
-2.9
-4.0
stress
DEANONOate 0.000625 M
-1.4
+0.4
-4.8
-1.6
-4.5
stress
DEANONOate 0.000625 M
+0.3
-1.1
-4.0
-0.6
-4.4
carbon source
octanoate (C)
-4.0
+0.4
-2.6
-2.2
-0.9
carbon source
D-Sorbitol (C)
-0.9
-0.6
-1.4
-1.5
-3.7
nitrogen source
nitrogen source nitrate 20mM
-2.7
-2.5
-1.2
-0.7
-1.0
nitrogen source
L-Alanine (N)
+0.4
-0.8
-1.3
-1.9
-4.0
stress
Lomefloxacin 0.0002 mM
+0.8
+0.2
-4.8
-1.5
-2.2
motility
outer cut, LB soft agar motility assay
+1.9
-0.4
-2.7
-2.1
-3.8
nitrogen source
L-Asparagine (N)
+0.2
+0.5
-2.2
-1.5
-3.8
stress
Vancomycin 0.24 mg/ml
-2.3
+0.3
-2.6
-2.4
+0.7
stress
Nalidixic 0.008 mg/ml
-0.7
+0.6
-3.2
-3.0
+0.1
lb
LB no stress 24 hours
+0.6
-0.3
-4.3
-0.7
-1.4
nitrogen source
Parabanic Acid (N)
+1.3
+0.3
-1.4
-3.6
-2.7
stress
Rifampicin 0.003 mg/ml
-3.0
+0.3
-2.2
-2.9
+2.1
lb
LB no stress 48 hours
+0.9
+0.4
-3.1
-1.1
-2.3
stress
Bile salts 0.0125 M
-0.1
-0.3
-2.1
-4.4
+1.7
stress
Calcium chloride 0.0125 M
+0.8
-1.0
-3.7
+0.4
-1.4
motility
inner cut, LB soft agar motility assay
+1.6
+0.3
-3.2
-2.8
-0.1
carbon source
L-Leucine (C)
+2.1
-0.4
-2.4
-0.9
-2.8
nitrogen source
L-Histidine (N)
+1.4
+0.6
-1.4
-1.0
-3.9
motility
outer cut, LB soft agar motility assay
+1.6
+0.6
-2.6
-3.2
-0.3
motility
inner cut, LB soft agar motility assay
+2.8
+0.9
-3.6
-2.3
-1.4
carbon source
L-Leucine (C)
+1.8
+0.5
-2.3
-0.3
-3.0
motility
inner cut, LB soft agar motility assay
+2.5
-1.0
-2.7
-3.2
+1.2
stress
Hydroxylamine 0.5 M
-2.9
-0.9
-1.7
+0.1
+2.5
carbon source
L-Histidine (C)
+2.4
-0.0
-1.3
+0.2
-3.8
stress
deoxycholate 0.0125 M
-0.1
+0.7
-2.0
-2.8
+1.7
stress
Choline Lysine 20 mM
+2.6
+0.6
-2.6
-0.4
-1.1
carbon source
D-Alanine (C)
+5.0
+0.2
-0.8
-0.5
-3.3
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