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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_4333 and Pf6N2E2_4334 are separated by 220 nucleotides
Pf6N2E2_4334 and Pf6N2E2_4335 overlap by 4 nucleotides
Pf6N2E2_4335 and Pf6N2E2_4336 are separated by 81 nucleotides
Pf6N2E2_4336 and Pf6N2E2_4337 are separated by 415 nucleotides
Pf6N2E2_4333 - Uncharacterized protein ImpD, at 5,055,464 to 5,055,952
_4333
Pf6N2E2_4334 - Uncharacterized protein ImpC, at 5,056,173 to 5,057,675
_4334
Pf6N2E2_4335 - Uncharacterized protein ImpB, at 5,057,672 to 5,058,178
_4335
Pf6N2E2_4336 - Uncharacterized protein ImpA, at 5,058,260 to 5,059,303
_4336
Pf6N2E2_4337 - Uncharacterized protein ImpI/VasC, at 5,059,719 to 5,061,218
_4337
Group
Condition
Pf6N2E2
_4333
Pf6N2E2
_4334
Pf6N2E2
_4335
Pf6N2E2
_4336
Pf6N2E2
_4337
stress
deoxycholate 0.0125 M
-0.3
+0.6
-0.3
-0.1
+0.3
motility
inner cut, LB soft agar motility assay
-0.5
+0.0
+0.1
+0.4
+0.8
stress
Hydroxylamine 0.5 M
+0.7
+0.9
-0.2
-0.2
+0.2
stress
RCH2 defined with copper (II) chloride 0.03 mM
+0.2
+0.3
+0.4
+0.1
+0.5
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+0.3
+0.5
+0.3
+0.4
+0.1
nitrogen source
nitrogen source nitrite 1mM
+0.0
+0.2
+0.7
+0.3
+0.4
nitrogen source
Putrescine (N)
+0.4
+0.4
+0.3
+0.4
+0.2
motility
outer cut, LB soft agar motility assay
+0.3
+0.2
+0.2
+0.3
+0.7
nitrogen source
L-Isoleucine (N)
+0.3
+0.4
+0.2
+0.4
+0.4
nitrogen source
L-Phenylalanine (N)
+0.5
+0.3
+0.3
+0.4
+0.3
motility
inner cut, LB soft agar motility assay
-0.2
+0.3
+0.4
+0.3
+1.0
stress
Aluminum chloride 4 mM
+0.6
+0.3
+0.4
+0.2
+0.2
nitrogen source
L-Phenylalanine (N)
+0.3
+0.3
+0.3
+0.4
+0.4
nitrogen source
L-Isoleucine (N)
+0.2
+0.3
+0.4
+0.6
+0.4
nitrogen source
Cytosine (N)
+0.3
+0.5
+0.3
+0.3
+0.5
motility
outer cut, LB soft agar motility assay
+0.1
+0.4
+0.0
+0.5
+1.0
carbon source
Carnitine Hydrochloride (C)
+0.4
+0.5
+0.5
+0.4
+0.4
stress
Doxycycline hyclate 0.0001 mg/ml
+0.3
+0.4
+0.7
+0.4
+0.6
carbon source
D-Glucose (C)
+0.5
+0.6
+0.3
+0.6
+0.6
motility
inner cut, LB soft agar motility assay
+0.5
+0.4
+0.5
+0.4
+0.9
nitrogen source
Cytidine (N)
+0.2
+0.5
+0.5
+0.6
+0.7
carbon source
Carbon source Sucrose 10 mM
+0.4
+0.5
+0.7
+0.5
+0.6
nitrogen source
Cytosine (N)
+0.7
+0.6
+0.7
+0.5
+0.6
carbon source
D-Sorbitol (C)
+0.5
+0.9
+0.8
+0.6
+0.7
carbon source
D-Glucose (C)
+0.7
+0.5
+0.9
+1.0
+0.6
nitrogen source
Cytidine (N)
+0.7
+0.9
+0.8
+0.6
+1.0
carbon source
Carbon source Sucrose 10 mM
+1.0
+0.8
+0.8
+0.9
+0.9
carbon source
D-Sorbitol (C)
+0.8
+1.0
+1.2
+0.8
+0.9
nitrogen source
L-Serine (N)
+0.6
+1.2
+0.9
+1.4
+1.1
nitrogen source
L-Serine (N)
+1.2
+0.9
+1.3
+0.7
+1.1
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