Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_4202 and Pf6N2E2_4203 are separated by 565 nucleotidesPf6N2E2_4203 and Pf6N2E2_4204 are separated by 5 nucleotidesPf6N2E2_4204 and Pf6N2E2_4205 are separated by 18 nucleotidesPf6N2E2_4205 and Pf6N2E2_4206 are separated by 9 nucleotides Pf6N2E2_4202 - GTPase and tRNA-U34 5-formylation enzyme TrmE, at 4,892,092 to 4,893,462 _4202 Pf6N2E2_4203 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 4,894,028 to 4,895,926 _4203 Pf6N2E2_4204 - rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB, at 4,895,932 to 4,896,567 _4204 Pf6N2E2_4205 - Chromosome (plasmid) partitioning protein ParA, at 4,896,586 to 4,897,383 _4205 Pf6N2E2_4206 - Chromosome (plasmid) partitioning protein ParB, at 4,897,393 to 4,898,265 _4206
Group Condition Pf6N2E2_4202 Pf6N2E2_4203 Pf6N2E2_4204 Pf6N2E2_4205 Pf6N2E2_4206
motility outer cut, LB soft agar motility assay N.D. N.D. -3.4 -3.6 -2.6
motility outer cut, LB soft agar motility assay N.D. N.D. -3.8 -2.0 -3.4
stress Bile salts 0.0125 M N.D. N.D. -1.7 -2.8 -4.3
motility inner cut, LB soft agar motility assay N.D. N.D. -3.1 -3.1 -2.4
stress deoxycholate 0.0125 M N.D. N.D. -3.0 -3.1 -2.3
stress DEANONOate 0.000625 M N.D. N.D. -3.6 -0.4 -4.3
stress 4-Hydroxyacetophenone 0.00025 M N.D. N.D. -2.7 -2.8 -2.7
carbon source D-Sorbitol (C) N.D. N.D. -0.9 -1.1 -6.1
stress pyrophosphate 0.0025 M N.D. N.D. -2.2 -0.4 -4.7
stress thiosulfate 0.25 M N.D. N.D. -2.9 -1.9 -2.0
carbon source D-Mannose (C) N.D. N.D. -2.0 -1.1 -3.5
nitrogen source D-Alanine (N) N.D. N.D. -2.1 -1.2 -3.2
stress Cisplatin 0.008 mg/ml N.D. N.D. -3.3 -1.6 -1.6
motility inner cut, LB soft agar motility assay N.D. N.D. -2.2 -2.3 -1.9
carbon source D-Xylose (C) N.D. N.D. -2.1 -1.3 -2.8
motility inner cut, LB soft agar motility assay N.D. N.D. -2.8 -1.3 -2.1
carbon source propionate (C) N.D. N.D. -4.0 -1.0 -1.1
stress phosphite 0.25 M N.D. N.D. -3.1 -1.1 -1.9
carbon source succinate (C) N.D. N.D. -2.5 -1.3 -2.3
carbon source D-Alanine (C) N.D. N.D. -1.6 -1.7 -2.7
lb LB no stress 48 hours N.D. N.D. -3.0 -1.0 -1.9
carbon source succinate (C) N.D. N.D. -2.1 -1.2 -2.5
stress DEANONOate 0.000625 M N.D. N.D. -3.8 -1.6 -0.2
stress Choline Lysine 20 mM N.D. N.D. -4.9 -0.5 -0.2
nitrogen source nitrogen source nitrate 20mM N.D. N.D. -1.3 -0.8 -3.5
carbon source D-Lactate (C) N.D. N.D. -2.9 -0.9 -1.9
nitrogen source Glycine (N) N.D. N.D. +0.1 -1.3 -4.2
stress molybdate 0.25 M N.D. N.D. -1.0 -3.2 -1.3
nitrogen source Parabanic Acid (N) N.D. N.D. -0.4 -1.5 -3.5
stress Nickel (II) chloride 0.0025 M N.D. N.D. +1.5 -0.2 -4.1
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