Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_4201 and Pf6N2E2_4202 are separated by 79 nucleotidesPf6N2E2_4202 and Pf6N2E2_4203 are separated by 565 nucleotidesPf6N2E2_4203 and Pf6N2E2_4204 are separated by 5 nucleotidesPf6N2E2_4204 and Pf6N2E2_4205 are separated by 18 nucleotides Pf6N2E2_4201 - Inner membrane protein translocase component YidC, long form, at 4,890,330 to 4,892,012 _4201 Pf6N2E2_4202 - GTPase and tRNA-U34 5-formylation enzyme TrmE, at 4,892,092 to 4,893,462 _4202 Pf6N2E2_4203 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 4,894,028 to 4,895,926 _4203 Pf6N2E2_4204 - rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB, at 4,895,932 to 4,896,567 _4204 Pf6N2E2_4205 - Chromosome (plasmid) partitioning protein ParA, at 4,896,586 to 4,897,383 _4205
Group Condition Pf6N2E2_4201 Pf6N2E2_4202 Pf6N2E2_4203 Pf6N2E2_4204 Pf6N2E2_4205
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -3.4 -3.6
motility inner cut, LB soft agar motility assay N.D. N.D. N.D. -3.1 -3.1
stress deoxycholate 0.0125 M N.D. N.D. N.D. -3.0 -3.1
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -3.8 -2.0
stress 4-Hydroxyacetophenone 0.00025 M N.D. N.D. N.D. -2.7 -2.8
stress Choline Lysine 20 mM N.D. N.D. N.D. -4.9 -0.5
stress DEANONOate 0.000625 M N.D. N.D. N.D. -3.8 -1.6
carbon source propionate (C) N.D. N.D. N.D. -4.0 -1.0
stress Cisplatin 0.008 mg/ml N.D. N.D. N.D. -3.3 -1.6
stress Cadmium chloride hemipentahydrate 0.000015625 M N.D. N.D. N.D. -3.9 -0.9
stress thiosulfate 0.25 M N.D. N.D. N.D. -2.9 -1.9
stress Hydroxide 0.05 M N.D. N.D. N.D. -3.0 -1.6
carbon source a-Ketoglutaric (C) N.D. N.D. N.D. -3.4 -1.1
motility inner cut, LB soft agar motility assay N.D. N.D. N.D. -2.2 -2.3
stress Bile salts 0.0125 M N.D. N.D. N.D. -1.7 -2.8
stress Hydroxylamine 0.5 M N.D. N.D. N.D. -2.0 -2.2
stress phosphite 0.25 M N.D. N.D. N.D. -3.1 -1.1
stress molybdate 0.25 M N.D. N.D. N.D. -1.0 -3.2
carbon source Putrescine (C) N.D. N.D. N.D. -3.2 -0.9
motility inner cut, LB soft agar motility assay N.D. N.D. N.D. -2.8 -1.3
lb LB no stress 48 hours N.D. N.D. N.D. -3.0 -1.0
stress DEANONOate 0.000625 M N.D. N.D. N.D. -3.6 -0.4
stress dimethyl sulfone 0.25 M N.D. N.D. N.D. -2.8 -1.1
stress Zinc chloride 0.0000015625 M N.D. N.D. N.D. -3.0 -0.9
stress Vanillyl Alcohol 0.005 M N.D. N.D. N.D. -3.1 -0.7
carbon source Putrescine (C) N.D. N.D. N.D. -2.8 -1.0
carbon source succinate (C) N.D. N.D. N.D. -2.5 -1.3
nitrogen source L-Citrulline (N) N.D. N.D. N.D. -2.0 -1.8
carbon source D-Lactate (C) N.D. N.D. N.D. -2.9 -0.9
stress Cobalt chloride 0.6 mM N.D. N.D. N.D. +2.2 -1.8
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