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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_4200 and Pf6N2E2_4201 are separated by 240 nucleotides
Pf6N2E2_4201 and Pf6N2E2_4202 are separated by 79 nucleotides
Pf6N2E2_4202 and Pf6N2E2_4203 are separated by 565 nucleotides
Pf6N2E2_4203 and Pf6N2E2_4204 are separated by 5 nucleotides
Pf6N2E2_4200 - Ribonuclease P protein component (EC 3.1.26.5), at 4,889,892 to 4,890,089
_4200
Pf6N2E2_4201 - Inner membrane protein translocase component YidC, long form, at 4,890,330 to 4,892,012
_4201
Pf6N2E2_4202 - GTPase and tRNA-U34 5-formylation enzyme TrmE, at 4,892,092 to 4,893,462
_4202
Pf6N2E2_4203 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA, at 4,894,028 to 4,895,926
_4203
Pf6N2E2_4204 - rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB, at 4,895,932 to 4,896,567
_4204
Group
Condition
Pf6N2E2
_4200
Pf6N2E2
_4201
Pf6N2E2
_4202
Pf6N2E2
_4203
Pf6N2E2
_4204
stress
Choline Lysine 20 mM
N.D.
N.D.
N.D.
N.D.
-4.9
carbon source
propionate (C)
N.D.
N.D.
N.D.
N.D.
-4.0
stress
Cadmium chloride hemipentahydrate 0.000015625 M
N.D.
N.D.
N.D.
N.D.
-3.9
stress
DEANONOate 0.000625 M
N.D.
N.D.
N.D.
N.D.
-3.8
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
N.D.
N.D.
-3.8
stress
DEANONOate 0.000625 M
N.D.
N.D.
N.D.
N.D.
-3.6
motility
outer cut, LB soft agar motility assay
N.D.
N.D.
N.D.
N.D.
-3.4
carbon source
a-Ketoglutaric (C)
N.D.
N.D.
N.D.
N.D.
-3.4
stress
Cisplatin 0.008 mg/ml
N.D.
N.D.
N.D.
N.D.
-3.3
carbon source
Putrescine (C)
N.D.
N.D.
N.D.
N.D.
-3.2
motility
inner cut, LB soft agar motility assay
N.D.
N.D.
N.D.
N.D.
-3.1
stress
Vanillyl Alcohol 0.005 M
N.D.
N.D.
N.D.
N.D.
-3.1
stress
phosphite 0.25 M
N.D.
N.D.
N.D.
N.D.
-3.1
stress
Hydroxide 0.05 M
N.D.
N.D.
N.D.
N.D.
-3.0
lb
LB no stress 48 hours
N.D.
N.D.
N.D.
N.D.
-3.0
stress
deoxycholate 0.0125 M
N.D.
N.D.
N.D.
N.D.
-3.0
stress
Zinc chloride 0.0000015625 M
N.D.
N.D.
N.D.
N.D.
-3.0
lb
LB no stress 48 hours
N.D.
N.D.
N.D.
N.D.
-3.0
nitrogen source
Glucuronamide (N)
N.D.
N.D.
N.D.
N.D.
-3.0
stress
Fusidic 0.4 mg/ml
N.D.
N.D.
N.D.
N.D.
-2.9
stress
thiosulfate 0.25 M
N.D.
N.D.
N.D.
N.D.
-2.9
carbon source
D-Lactate (C)
N.D.
N.D.
N.D.
N.D.
-2.9
stress
dimethyl sulfone 0.25 M
N.D.
N.D.
N.D.
N.D.
-2.8
carbon source
Putrescine (C)
N.D.
N.D.
N.D.
N.D.
-2.8
motility
inner cut, LB soft agar motility assay
N.D.
N.D.
N.D.
N.D.
-2.8
stress
perchlorate 0.00625 M
N.D.
N.D.
N.D.
N.D.
-2.7
stress
4-Hydroxyacetophenone 0.00025 M
N.D.
N.D.
N.D.
N.D.
-2.7
stress
perchlorate 0.00625 M
N.D.
N.D.
N.D.
N.D.
-2.6
stress
Cobalt chloride 0.6 mM
N.D.
N.D.
N.D.
N.D.
+2.6
stress
Cobalt chloride 0.4 mM
N.D.
N.D.
N.D.
N.D.
+2.8
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