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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_4027 and Pf6N2E2_4028 are separated by 67 nucleotides
Pf6N2E2_4028 and Pf6N2E2_4029 overlap by 4 nucleotides
Pf6N2E2_4029 and Pf6N2E2_4030 are separated by 1 nucleotides
Pf6N2E2_4030 and Pf6N2E2_4031 are separated by 12 nucleotides
Pf6N2E2_4027 - Ferredoxin reductase, at 4,698,874 to 4,700,406
_4027
Pf6N2E2_4028 - Biofilm PGA synthesis auxiliary protein PgaD, at 4,700,474 to 4,700,980
_4028
Pf6N2E2_4029 - Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-), at 4,700,977 to 4,702,332
_4029
Pf6N2E2_4030 - Biofilm PGA synthesis deacetylase PgaB (EC 3.-), at 4,702,334 to 4,704,331
_4030
Pf6N2E2_4031 - Biofilm PGA outer membrane secretin PgaA, at 4,704,344 to 4,706,824
_4031
Group
Condition
Pf6N2E2
_4027
Pf6N2E2
_4028
Pf6N2E2
_4029
Pf6N2E2
_4030
Pf6N2E2
_4031
nitrogen source
L-Glutamic (N)
-0.1
+0.4
+0.3
+0.4
+0.4
carbon source
D-Glucose (C)
-0.1
+0.6
+0.4
+0.2
+0.3
nitrogen source
Parabanic Acid (N)
-0.2
+0.5
+0.2
+0.5
+0.4
carbon source
L-Alanine (C)
-0.2
+0.3
+0.7
+0.2
+0.5
stress
Cobalt chloride 0.16 mM
-0.0
+0.5
+0.4
+0.4
+0.3
carbon source
D-Glucose (C)
-0.2
+0.6
+0.6
+0.5
+0.4
carbon source
RCH2_defined
+0.2
+0.3
+0.7
+0.5
+0.3
stress
Nickel (II) chloride 1.5 mM
+0.2
+0.6
+0.4
+0.5
+0.4
carbon source
4-Hydroxybenzoic Acid (C)
+0.0
+0.6
+0.5
+0.4
+0.4
nitrogen source
L-Alanine (N)
+0.2
+0.3
+0.8
+0.5
+0.4
stress
RCH2 defined with Nickel (II) chloride 0.04 mM
+0.1
+0.3
+0.8
+0.5
+0.6
stress
Cobalt chloride 0.6 mM
-0.0
+0.5
+0.6
+0.7
+0.5
stress
Nickel (II) chloride 1.5 mM
+0.1
+0.6
+0.7
+0.5
+0.4
nitrogen source
L-Alanine (N)
-0.1
+0.5
+0.7
+0.7
+0.6
stress
RCH2 defined with Nickel (II) chloride 0.04 mM
+0.1
+0.4
+0.7
+0.6
+0.6
nitrogen source
L-Isoleucine (N)
+0.0
+0.5
+0.8
+0.6
+0.5
nitrogen source
L-Glutamine (N)
+0.0
+0.5
+0.6
+0.8
+0.6
carbon source
4-Hydroxybenzoic Acid (C)
+0.3
+0.6
+0.6
+0.6
+0.5
nitrogen source
L-Isoleucine (N)
-0.0
+0.6
+0.6
+0.8
+0.6
stress
Cobalt chloride 0.6 mM
+0.0
+0.8
+0.9
+0.6
+0.6
stress
Fusidic 0.3 mg/ml
+0.1
+0.8
+0.8
+0.7
+0.7
stress
RCH2 defined with copper (II) chloride 0.03 mM
+0.1
+0.5
+1.0
+0.8
+0.7
nitrogen source
Glucuronamide (N)
-0.1
+0.7
+1.0
+0.8
+0.8
stress
RCH2 defined with copper (II) chloride 0.03 mM
+0.1
+0.8
+1.0
+0.7
+0.8
stress
Cobalt chloride 0.6 mM
-0.2
+1.1
+1.1
+1.4
+1.1
nitrogen source
L-Serine (N)
+0.1
+1.1
+1.0
+1.3
+1.2
stress
Doxycycline hyclate 0.0001 mg/ml
-0.0
+1.2
+1.6
+0.9
+1.0
nitrogen source
Cytidine (N)
-0.1
+1.3
+1.5
+1.4
+1.4
nitrogen source
L-Serine (N)
-0.4
+1.4
+1.9
+1.7
+1.5
nitrogen source
Cytidine (N)
+0.1
+1.4
+1.6
+1.4
+1.5
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