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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_3945 and Pf6N2E2_3946 are separated by 49 nucleotides
Pf6N2E2_3946 and Pf6N2E2_3947 are separated by 115 nucleotides
Pf6N2E2_3947 and Pf6N2E2_3948 are separated by 86 nucleotides
Pf6N2E2_3948 and Pf6N2E2_3949 are separated by 204 nucleotides
Pf6N2E2_3945 - Prolipoprotein diacylglyceryl transferase (EC 2.4.99.-), at 4,582,737 to 4,583,546
_3945
Pf6N2E2_3946 - Thymidylate synthase (EC 2.1.1.45), at 4,583,596 to 4,584,567
_3946
Pf6N2E2_3947 - Cd(II)/Pb(II)-responsive transcriptional regulator, at 4,584,683 to 4,585,129
_3947
Pf6N2E2_3948 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 4,585,216 to 4,587,435
_3948
Pf6N2E2_3949 - RND efflux transporter, at 4,587,640 to 4,590,687
_3949
Group
Condition
Pf6N2E2
_3945
Pf6N2E2
_3946
Pf6N2E2
_3947
Pf6N2E2
_3948
Pf6N2E2
_3949
stress
Zinc chloride 0.0000015625 M
N.D.
N.D.
-4.9
-4.5
+0.0
stress
copper (II) chloride 3 mM
N.D.
N.D.
-1.6
-6.1
+0.0
stress
Cadmium chloride hemipentahydrate 0.000015625 M
N.D.
N.D.
-3.5
-3.6
+0.0
carbon source
Trisodium citrate (C)
N.D.
N.D.
-2.9
-3.8
+0.0
stress
Cadmium chloride hemipentahydrate 0.000015625 M
N.D.
N.D.
-2.0
-3.4
+0.0
stress
RCH2 defined with Zinc 0.032 mM
N.D.
N.D.
-0.1
-4.8
+0.0
carbon source
Trisodium citrate (C)
N.D.
N.D.
-1.0
-3.7
-0.1
stress
Cadmium chloride hemipentahydrate 0.00005 M
N.D.
N.D.
-0.4
-4.0
-0.1
stress
RCH2 defined with Zinc 0.032 mM
N.D.
N.D.
+0.2
-4.3
-0.0
stress
Nickel (II) chloride 0.0025 M
N.D.
N.D.
+0.9
-4.3
+0.0
stress
Bile salts 0.0125 M
N.D.
N.D.
-3.0
-0.4
+0.1
stress
Gjerstad humics 0.5 M
N.D.
N.D.
+0.4
-3.7
+0.1
nitrogen source
L-Serine (N)
N.D.
N.D.
-2.0
-0.3
-0.1
stress
RCH2 defined with Zinc 0.016 mM
N.D.
N.D.
-0.9
-1.4
+0.1
stress
Gjerstad humics 0.5 M
N.D.
N.D.
+0.6
-2.7
+0.1
stress
Nickel (II) chloride 0.0025 M
N.D.
N.D.
+1.1
-3.2
+0.1
nitrogen source
L-Glutamic (N)
N.D.
N.D.
-1.7
-0.1
-0.0
stress
Cobalt chloride 0.6 mM
N.D.
N.D.
-0.7
-1.1
+0.0
stress
dimethyl sulfone 0.25 M
N.D.
N.D.
-1.5
-0.3
+0.1
stress
RCH2 defined with Cobalt chloride 0.04 mM
N.D.
N.D.
-0.3
-1.3
-0.1
nitrogen source
L-Asparagine (N)
N.D.
N.D.
-1.8
+0.2
-0.0
lb
LB no stress 48 hours
N.D.
N.D.
-0.8
-0.8
+0.1
stress
RCH2 defined with Zinc 0.016 mM
N.D.
N.D.
+0.2
-1.7
+0.1
carbon source
octanoate (C)
N.D.
N.D.
-1.4
+0.1
+0.1
lb
LB no stress 48 hours
N.D.
N.D.
+0.7
-0.8
-0.1
lb
LB
N.D.
N.D.
+0.8
-1.0
+0.1
lb
LB no stress 48 hours
N.D.
N.D.
+1.1
-0.9
+0.0
lb
LB no stress 24 hours
N.D.
N.D.
+1.2
-0.5
+0.0
stress
DEANONOate 0.000625 M
N.D.
N.D.
+1.7
-0.1
-0.2
stress
Nickel (II) chloride 1.5 mM
N.D.
N.D.
+1.1
+0.3
+0.1
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