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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_3806 and Pf6N2E2_3807 overlap by 4 nucleotides
Pf6N2E2_3807 and Pf6N2E2_3808 are separated by 104 nucleotides
Pf6N2E2_3808 and Pf6N2E2_3809 are separated by 121 nucleotides
Pf6N2E2_3809 and Pf6N2E2_3810 are separated by 109 nucleotides
Pf6N2E2_3806 - Conserved hypothetical protein (perhaps related to histidine degradation), at 4,435,983 to 4,436,552
_3806
Pf6N2E2_3807 - Histidine utilization repressor, at 4,436,549 to 4,437,298
_3807
Pf6N2E2_3808 - Formiminoglutamic iminohydrolase (EC 3.5.3.13)
(from data)
, at 4,437,403 to 4,438,767
_3808
Pf6N2E2_3809 - Outer membrane lipoprotein Blc, at 4,438,889 to 4,439,455
_3809
Pf6N2E2_3810 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,439,565 to 4,439,819
_3810
Group
Condition
Pf6N2E2
_3806
Pf6N2E2
_3807
Pf6N2E2
_3808
Pf6N2E2
_3809
Pf6N2E2
_3810
nitrogen source
L-Histidine (N)
-0.4
+0.3
-3.8
+0.2
-0.3
nitrogen source
L-Histidine (N)
-0.3
+0.5
-3.7
-0.0
-0.5
denitrifying
Nitrate reduction, replicate B
+0.2
-4.2
-0.1
+0.1
+0.2
carbon source
L-Histidine (C)
-0.1
+0.9
-4.0
+0.1
-0.6
carbon source
L-Histidine (C)
+0.0
+1.0
-4.5
+0.0
-0.1
stress
antimonate 0.005 M
-0.3
-3.0
-0.3
-0.0
+0.2
motility
outer cut, LB soft agar motility assay
-0.5
-2.9
-0.1
+0.1
+0.4
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.6
-1.7
+0.1
+0.1
-0.8
nitrogen source
nitrogen source nitrite 5mM
-0.2
-2.1
-0.1
-0.0
-0.4
motility
inner cut, LB soft agar motility assay
-0.1
-1.1
-0.5
-0.1
-1.0
motility
inner cut, LB soft agar motility assay
-0.2
-2.5
-0.3
-0.2
+0.4
nitrogen source
Glycine (N)
-0.5
-1.7
+0.1
-0.0
-0.6
nitrogen source
nitrogen source nitrite 1mM
-0.3
-0.8
-0.4
+0.0
-1.1
carbon source
Ying_AminoAcid20 rep B; time point 3
-0.3
+0.1
-2.3
-0.1
+0.2
carbon source
Ying_AminoAcid20 rep A; time point 3
-0.3
-0.1
-2.3
+0.0
+0.2
denitrifying
Nitrate reduction, replicate A
+0.4
-2.4
-0.1
+0.1
-0.3
vitamin
No vitamins t1
-2.2
-0.5
+0.1
+0.2
+0.2
motility
outer cut, LB soft agar motility assay
-0.3
-1.9
-0.2
-0.1
+0.3
stress
deoxycholate 0.0125 M
-2.2
-0.7
+0.4
+0.1
+0.3
nitrogen source
L-Serine (N)
-0.2
-2.4
+0.0
-0.1
+0.6
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
-0.6
-1.5
+0.2
+0.1
-0.2
stress
periodate 0.00125 M
-0.5
-2.6
+0.2
-0.1
+1.0
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
+0.4
-2.1
+0.0
+0.3
-0.4
carbon source
Ying_AminoAcid20 rep C; time point 3
+0.1
+0.1
-2.2
-0.0
+0.3
carbon source
Ying_AminoAcid20 rep A; time point 2
-0.5
+0.5
-0.8
+0.3
-1.1
nitrogen source
nitrogen source nitrite 0.5mM
+0.8
-1.6
-0.0
-0.3
-0.4
stress
Hydroxylamine 0.5 M
-1.1
+1.1
-0.1
-0.7
-0.2
nitrogen source
nitrogen source nitrite 5mM
+0.8
-1.5
+0.3
-0.0
+0.1
carbon source
D-Galactose (C)
+0.3
+2.3
-0.1
+0.1
-0.4
carbon source
D-Galactose (C)
+0.2
+2.6
+0.1
+0.2
-0.2
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