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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_3539 and Pf6N2E2_3540 are separated by 375 nucleotides
Pf6N2E2_3540 and Pf6N2E2_3541 are separated by 57 nucleotides
Pf6N2E2_3541 and Pf6N2E2_3542 are separated by 245 nucleotides
Pf6N2E2_3542 and Pf6N2E2_3543 are separated by 203 nucleotides
Pf6N2E2_3539 - FIG00955840: hypothetical protein, at 4,129,902 to 4,130,027
_3539
Pf6N2E2_3540 - Copper metallochaperone, bacterial analog of Cox17 protein, at 4,130,403 to 4,130,555
_3540
Pf6N2E2_3541 - Putative multicopper oxidases, at 4,130,613 to 4,131,011
_3541
Pf6N2E2_3542 - Outer membrane receptor proteins, mostly Fe transport, at 4,131,257 to 4,133,383
_3542
Pf6N2E2_3543 - Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB, at 4,133,587 to 4,134,966
_3543
Group
Condition
Pf6N2E2
_3539
Pf6N2E2
_3540
Pf6N2E2
_3541
Pf6N2E2
_3542
Pf6N2E2
_3543
nitrogen source
nitrogen source nitrate 20mM
-3.8
N.D.
-0.4
-0.5
+0.3
motility
inner cut, LB soft agar motility assay
-1.3
N.D.
-0.4
-0.6
-0.6
nitrogen source
nitrogen source nitrite 1mM
-1.2
N.D.
-0.9
-0.4
+0.3
nitrogen source
nitrogen source nitrite 1mM
-1.3
N.D.
-0.5
-0.5
+0.2
motility
inner cut, LB soft agar motility assay
-0.8
N.D.
-0.3
-0.4
-0.2
motility
inner cut, LB soft agar motility assay
+0.1
N.D.
-1.2
-0.6
+0.1
nitrogen source
nitrogen source nitrate 5mM
-1.2
N.D.
-0.2
-0.4
+0.3
stress
Vanillyl Alcohol 0.005 M
-0.0
N.D.
-0.7
-0.9
+0.3
nitrogen source
nitrogen source Ammonium chloride 20mM
-0.5
N.D.
-0.4
-0.4
+0.4
lb
LB no stress 24 hours
+0.3
N.D.
-0.9
-0.2
+0.2
stress
dimethyl sulfone 0.25 M
+0.1
N.D.
-0.2
-1.0
+0.6
carbon source
octanoate (C)
-1.1
N.D.
+0.2
+0.3
+0.3
stress
Nickel (II) chloride 0.0025 M
-0.0
N.D.
-1.0
+0.3
+0.5
stress
4-Hydroxyacetophenone 0.00025 M
+0.4
N.D.
-0.1
-0.9
+0.7
nitrogen source
nitrogen source nitrite 5mM
+1.1
N.D.
-0.4
-0.4
+0.3
carbon source
Carbon source D,L-Lactate 10 mM
+1.1
N.D.
-0.3
+0.1
-0.2
nitrogen source
nitrogen source nitrite 5mM
+1.5
N.D.
-0.6
-0.5
+0.5
stress
pyrophosphate 0.0025 M
-0.3
N.D.
+0.7
+0.4
+0.3
stress
Magnesium chloride 0.125 M
+1.0
N.D.
+0.3
+0.4
-0.2
stress
DEANONOate 0.000625 M
+1.0
N.D.
+0.5
-1.5
+1.4
stress
deoxycholate 0.0125 M
+0.5
N.D.
+0.4
+0.3
+0.7
stress
sodium iodate 0.005 M
-0.1
N.D.
+1.0
+0.3
+0.6
stress
Choline Lysine 20 mM
+0.4
N.D.
+0.7
+0.5
+0.4
stress
Phosphomycin 0.004 mg/ml
+1.2
N.D.
+0.3
+0.5
+0.1
stress
DEANONOate 0.000625 M
+1.6
N.D.
+0.7
-1.4
+1.3
stress
Gjerstad humics 0.5 M
+0.2
N.D.
+0.9
+0.8
+0.3
stress
chromate 0.0000015625 M
+0.7
N.D.
+0.9
+0.5
+0.1
stress
Sisomicin 0.0016 mg/ml
+0.7
N.D.
+0.8
+0.7
+0.4
stress
chromate 0.0000015625 M
+0.5
N.D.
+1.2
+1.1
+0.1
stress
Hydroxylamine 0.5 M
+0.9
N.D.
+2.3
+1.2
-0.1
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