Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_3511 and Pf6N2E2_3512 are separated by 17 nucleotidesPf6N2E2_3512 and Pf6N2E2_3513 are separated by 81 nucleotidesPf6N2E2_3513 and Pf6N2E2_3514 are separated by 193 nucleotidesPf6N2E2_3514 and Pf6N2E2_3515 are separated by 30 nucleotides Pf6N2E2_3511 - Biotin carboxyl carrier protein of acetyl-CoA carboxylase, at 4,092,870 to 4,093,334 _3511 Pf6N2E2_3512 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 4,093,352 to 4,094,707 _3512 Pf6N2E2_3513 - hypothetical protein, at 4,094,789 to 4,098,757 _3513 Pf6N2E2_3514 - Ribosomal protein L11 methyltransferase (EC 2.1.1.-), at 4,098,951 to 4,099,829 _3514 Pf6N2E2_3515 - Probable transmembrane protein, at 4,099,860 to 4,100,942 _3515
Group Condition Pf6N2E2_3511 Pf6N2E2_3512 Pf6N2E2_3513 Pf6N2E2_3514 Pf6N2E2_3515
stress deoxycholate 0.0125 M N.D. N.D. +0.0 -0.9 -2.9
stress Benzalkonium Chloride 0.016 mg/ml N.D. N.D. +0.0 -1.1 -2.6
stress Vancomycin 0.24 mg/ml N.D. N.D. +0.1 -1.2 -2.2
stress Rifampicin 0.003 mg/ml N.D. N.D. -0.0 -1.2 -2.0
stress Chromium(III) Chloride Hexahydrate 0.000390625 M N.D. N.D. +0.1 -1.4 -1.4
stress Benzethonium chloride 0.02 mM N.D. N.D. +0.0 -0.6 -2.1
stress Bile salts 0.0125 M N.D. N.D. +0.0 +0.3 -2.9
stress Vancomycin 0.12 mg/ml N.D. N.D. +0.0 -0.8 -1.8
stress RCH2 defined with Nickel (II) chloride 0.04 mM N.D. N.D. +0.0 -2.4 -0.1
motility outer cut, LB soft agar motility assay N.D. N.D. +0.0 -1.0 -1.5
stress RCH2 defined with Cobalt chloride 0.02 mM N.D. N.D. +0.0 -2.2 -0.2
stress Doxycycline hyclate 0.0001 mg/ml N.D. N.D. +0.0 -0.1 -2.2
motility inner cut, LB soft agar motility assay N.D. N.D. +0.1 -0.0 -2.0
vitamin No vitamins t2 N.D. N.D. +0.2 -2.2 +0.2
nitrogen source L-Proline (N) N.D. N.D. +0.1 -0.5 -1.3
stress Tetracycline 0.0002 mg/ml N.D. N.D. -0.0 -0.5 -1.0
carbon source D-Galactose (C) N.D. N.D. +0.0 -0.6 -0.9
motility inner cut, LB soft agar motility assay N.D. N.D. +0.0 +0.1 -1.6
carbon source Trisodium citrate (C) N.D. N.D. +0.0 -1.5 +0.3
motility inner cut, LB soft agar motility assay N.D. N.D. +0.2 +0.3 -1.5
stress Fusidic 0.3 mg/ml N.D. N.D. +0.0 +0.4 -1.4
motility outer cut, LB soft agar motility assay N.D. N.D. +0.2 +0.6 -1.0
stress Cobalt chloride 0.6 mM N.D. N.D. +0.2 +0.6 -0.9
stress Cobalt chloride 0.6 mM N.D. N.D. +0.1 +0.8 -0.9
stress Fusidic 0.4 mg/ml N.D. N.D. -0.1 +1.0 -0.8
lb LB no stress 48 hours N.D. N.D. +0.1 +1.0 -0.4
carbon source Putrescine (C) N.D. N.D. -0.1 +0.5 +1.0
carbon source Ying_sugar7 rep C; time point 2 N.D. N.D. +0.0 +0.8 +0.7
carbon source Ying_sugar7 rep B; time point 2 N.D. N.D. -0.0 +0.9 +0.7
stress RCH2 defined with Cobalt chloride 0.04 mM N.D. N.D. +0.1 +1.2 +0.3
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