Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_3490 and Pf6N2E2_3491 are separated by 79 nucleotidesPf6N2E2_3491 and Pf6N2E2_3492 overlap by 14 nucleotidesPf6N2E2_3492 and Pf6N2E2_3493 are separated by 194 nucleotidesPf6N2E2_3493 and Pf6N2E2_3494 are separated by 2 nucleotides Pf6N2E2_3490 - Transcriptional regulator, AraC family, at 4,071,447 to 4,072,238 _3490 Pf6N2E2_3491 - Sodium/bile acid symporter family, at 4,072,318 to 4,073,346 _3491 Pf6N2E2_3492 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 4,073,333 to 4,074,610 _3492 Pf6N2E2_3493 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 4,074,805 to 4,076,157 _3493 Pf6N2E2_3494 - Acyl dehydratase, at 4,076,160 to 4,077,014 _3494
Group Condition Pf6N2E2_3490 Pf6N2E2_3491 Pf6N2E2_3492 Pf6N2E2_3493 Pf6N2E2_3494
stress Bile salts 0.0125 M -0.3 -0.9 -0.6 -0.9 -0.4
carbon source Ying_all64 rep A; time point 3 -0.2 -0.0 -0.4 +0.1 -1.9
carbon source Ying_AminoAcid20 rep B; time point 3 +0.1 -0.0 -0.3 +0.3 -2.0
stress RCH2 defined with copper (II) chloride 0.03 mM -0.4 -0.2 -0.1 -0.7 -0.5
carbon source D-Fructose (C) -0.3 -0.6 +0.0 -0.3 -0.5
carbon source D-Galactose (C) -0.5 -0.0 -0.1 -0.4 -0.6
stress Lomefloxacin 0.0002 mM +0.0 -0.2 +0.2 -1.5 -0.0
stress RCH2 defined with Nickel (II) chloride 0.04 mM -0.4 -0.3 +0.4 -0.1 -1.0
lb LB no stress 48 hours +0.2 -0.3 -0.6 -0.6 +0.0
stress deoxycholate 0.0125 M -0.1 -0.3 -0.7 +0.3 -0.2
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine -0.4 -0.1 -0.5 +0.7 -0.1
motility inner cut, LB soft agar motility assay +0.0 -0.2 -0.9 +0.4 +0.2
stress dimethyl sulfone 0.25 M -0.1 +0.1 -0.9 +0.5 +0.0
stress Vanillyl Alcohol 0.005 M -0.2 -0.8 -0.1 +0.5 +0.3
stress molybdate 0.25 M -0.4 -0.1 +0.4 -0.4 +0.4
vitamin With vitamins t1 +0.1 +0.0 -0.1 +0.8 -0.7
stress Nickel (II) chloride 0.0025 M -0.2 -0.0 -0.4 +0.9 -0.1
carbon source Ying_Others16 rep A; time point 3 +0.0 -0.3 +0.1 +0.8 -0.4
stress Doxycycline hyclate 0.0001 mg/ml +0.3 -0.1 +0.5 -0.7 +0.4
stress Cobalt chloride 0.4 mM -0.2 -0.3 -0.2 +0.9 +0.1
stress A22 0.002 mg/ml +0.2 +0.1 +0.3 -0.6 +0.5
stress Cobalt chloride 0.4 mM +0.1 -0.2 -0.2 +1.1 -0.2
stress Nickel (II) chloride 0.0025 M +0.1 -0.0 -0.5 +1.2 -0.1
nitrogen source Glucuronamide (N) -0.5 -0.0 +0.3 -0.1 +1.1
nitrogen source L-Serine (N) -0.2 +0.4 +0.4 -0.2 +0.6
carbon source Ying_sugar7 rep B; time point 2 -0.0 +0.1 +0.6 +0.1 +0.7
stress Cobalt chloride 0.6 mM +0.1 -0.1 -0.4 +1.5 +0.4
stress Cobalt chloride 0.6 mM +0.2 -0.0 -0.4 +1.6 +0.2
stress Cobalt chloride 0.6 mM -0.0 -0.2 -0.3 +1.7 +0.7
stress Cobalt chloride 0.6 mM +0.1 -0.1 -0.7 +1.9 +0.6
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