Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_3486 and Pf6N2E2_3487 are separated by 104 nucleotidesPf6N2E2_3487 and Pf6N2E2_3488 overlap by 4 nucleotidesPf6N2E2_3488 and Pf6N2E2_3489 are separated by 291 nucleotidesPf6N2E2_3489 and Pf6N2E2_3490 are separated by 239 nucleotides Pf6N2E2_3486 - FIG00953575: hypothetical protein, at 4,066,731 to 4,067,114 _3486 Pf6N2E2_3487 - Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4), at 4,067,219 to 4,069,612 _3487 Pf6N2E2_3488 - Cu(I)-responsive transcriptional regulator, at 4,069,609 to 4,070,022 _3488 Pf6N2E2_3489 - FIG00956297: hypothetical protein, at 4,070,314 to 4,071,207 _3489 Pf6N2E2_3490 - Transcriptional regulator, AraC family, at 4,071,447 to 4,072,238 _3490
Group Condition Pf6N2E2_3486 Pf6N2E2_3487 Pf6N2E2_3488 Pf6N2E2_3489 Pf6N2E2_3490
stress copper (II) chloride 3 mM +0.2 -5.5 -5.5 -0.0 -0.0
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B +0.4 -4.5 -0.6 +0.4 -0.3
vitamin With vitamins t2 -0.1 -3.5 -0.6 -0.1 +0.1
vitamin No vitamins t2 +0.4 -3.6 -0.8 -0.2 +0.1
stress RCH2 defined with Cobalt chloride 0.04 mM -0.1 -3.0 -0.6 -0.6 +0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A +0.3 -4.0 -0.4 +0.2 -0.0
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C +0.4 -4.1 -0.3 +0.2 -0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A +0.2 -3.7 -0.1 +0.2 -0.3
stress Zinc chloride 0.0000015625 M -0.0 -2.7 -0.9 +0.1 +0.2
lb LB no stress 48 hours -0.0 -2.2 -1.2 +0.0 +0.1
stress RCH2 defined with Cobalt chloride 0.04 mM +0.0 -2.7 -0.5 -0.2 +0.4
motility inner cut, LB soft agar motility assay -2.0 -0.2 -0.5 +0.1 -0.0
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B +0.2 -2.5 -0.3 +0.1 -0.1
carbon source Ying_OrganicAcid7 rep A; time point 2 -0.8 -0.1 -1.5 -0.1 -0.0
motility inner cut, LB soft agar motility assay -2.4 -0.3 -0.1 +0.2 +0.0
carbon source Ying_AminoAcid20 rep A; time point 2 -1.2 -0.0 -0.6 -0.2 -0.0
carbon source Ying_OrganicAcid7 rep B; time point 3 -0.4 -0.6 -0.9 +0.0 +0.0
stress RCH2 defined with copper (II) chloride 0.03 mM +0.4 -1.2 -0.6 +0.0 -0.4
motility outer cut, LB soft agar motility assay -1.8 +0.2 +0.1 -0.1 -0.3
denitrifying Nitrate reduction, replicate C -0.0 -1.5 +0.1 -0.2 -0.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C +0.3 -2.0 -0.0 +0.1 -0.1
denitrifying Nitrate reduction, replicate B +0.2 -1.6 +0.2 +0.3 -0.4
stress RCH2 defined with copper (II) chloride 0.03 mM +0.3 -1.3 -0.2 +0.1 +0.0
denitrifying Nitrate reduction, replicate A +0.2 -1.6 +0.1 +0.4 -0.1
stress RCH2 defined with Zinc 0.032 mM +0.4 -1.4 -0.2 -0.2 +0.2
carbon source Ying_AminoAcid20 rep C; time point 3 +0.5 -0.7 -0.2 -0.2 -0.2
carbon source m-Inositol (C) -0.3 +0.6 -0.2 +0.6 -0.4
carbon source D-Fructose (C) +0.4 -0.0 +0.4 +0.8 -0.3
carbon source D-Galactose (C) -0.3 +1.0 +0.2 +1.2 -0.5
carbon source D-Galactose (C) +0.0 +1.0 -0.1 +0.8 -0.3
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