Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_3339 and Pf6N2E2_3340 are separated by 288 nucleotidesPf6N2E2_3340 and Pf6N2E2_3341 are separated by 20 nucleotidesPf6N2E2_3341 and Pf6N2E2_3342 are separated by 131 nucleotidesPf6N2E2_3342 and Pf6N2E2_3343 are separated by 319 nucleotides Pf6N2E2_3339 - Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9), at 3,863,414 to 3,866,278 _3339 Pf6N2E2_3340 - Fructose repressor FruR, LacI family, at 3,866,567 to 3,867,562 _3340 Pf6N2E2_3341 - Putative deoxyribonuclease YjjV, at 3,867,583 to 3,868,359 _3341 Pf6N2E2_3342 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 3,868,491 to 3,870,521 _3342 Pf6N2E2_3343 - AmpE protein, at 3,870,841 to 3,871,677 _3343
Group Condition Pf6N2E2_3339 Pf6N2E2_3340 Pf6N2E2_3341 Pf6N2E2_3342 Pf6N2E2_3343
stress periodate 0.00125 M +0.1 -0.5 -3.5 +0.1 -0.2
nitrogen source L-Isoleucine (N) -0.4 -0.9 -0.1 -0.2 -0.6
nitrogen source Ammonium chloride (N) -0.1 -2.0 +0.1 +0.1 -0.0
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -0.1 -1.7 -0.0 +0.0 -0.0
nitrogen source L-Arginine (N) +0.2 -1.9 -0.1 -0.1 +0.1
vitamin With vitamins t2 -0.1 -1.3 -0.0 -0.1 -0.3
motility inner cut, LB soft agar motility assay -0.6 -0.8 -0.2 +0.3 -0.5
motility inner cut, LB soft agar motility assay -0.2 -0.7 -0.0 +0.1 -0.9
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C -0.2 -1.6 -0.1 -0.1 +0.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B -0.1 -1.5 -0.1 -0.2 +0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B -0.1 -1.6 -0.1 +0.0 +0.2
carbon source Ying_Nucleobase14 rep B; time point 1 +0.1 -0.2 -0.1 -0.6 -0.8
nitrogen source L-Arginine (N) +0.1 -1.7 +0.0 +0.1 -0.1
motility inner cut, LB soft agar motility assay -0.5 -0.5 -0.2 +0.1 -0.4
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A +0.0 -1.5 +0.2 -0.1 +0.0
motility outer cut, LB soft agar motility assay -0.2 -0.6 +0.4 -0.0 -0.7
nitrogen source nitrogen source Ammonium chloride 20mM +0.3 -2.2 -0.0 +0.5 +0.2
nitrogen source nitrogen source Ammonium chloride 20mM -0.0 -1.4 -0.0 +0.2 +0.2
stress deoxycholate 0.0125 M -1.0 +0.2 +0.1 -0.3 +0.2
carbon source L-Leucine (C) -0.1 -1.1 -0.2 +0.3 +0.2
nitrogen source nitrogen source nitrite 5mM -0.2 -0.9 +0.5 +0.0 -0.1
carbon source L-Leucine (C) +0.4 -1.2 -0.1 +0.2 +0.2
nitrogen source nitrogen source nitrite 5mM -0.2 -1.0 +0.2 +0.3 +0.3
nitrogen source L-Citrulline (N) +0.3 -1.0 -0.2 +0.2 +0.4
nitrogen source L-Serine (N) -0.3 -0.3 +0.2 -0.6 +0.7
stress 4-Hydroxyacetophenone 0.00025 M +0.5 -0.7 +0.0 -0.2 +0.4
carbon source D-Glucosamine Hydrochloride (C) +2.1 -0.6 -0.2 -0.1 +0.2
carbon source D-Fructose (C) +1.0 +1.6 -0.2 -0.1 +0.3
carbon source D-Mannose (C) +4.6 -0.6 +0.2 +0.1 -0.1
carbon source D-Mannose (C) +5.4 -0.3 +0.1 +0.1 +0.1
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