Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_3182 and Pf6N2E2_3183 are separated by 88 nucleotidesPf6N2E2_3183 and Pf6N2E2_3184 are separated by 13 nucleotidesPf6N2E2_3184 and Pf6N2E2_3185 are separated by 3 nucleotidesPf6N2E2_3185 and Pf6N2E2_3186 are separated by 50 nucleotides Pf6N2E2_3182 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 3,678,532 to 3,678,957 _3182 Pf6N2E2_3183 - Believed to be involved in assembly of Fe-S clusters, at 3,679,046 to 3,679,246 _3183 Pf6N2E2_3184 - Ferredoxin, 2Fe-2S, at 3,679,260 to 3,679,601 _3184 Pf6N2E2_3185 - Chaperone protein HscA, at 3,679,605 to 3,681,467 _3185 Pf6N2E2_3186 - Chaperone protein HscB, at 3,681,518 to 3,682,039 _3186
Group Condition Pf6N2E2_3182 Pf6N2E2_3183 Pf6N2E2_3184 Pf6N2E2_3185 Pf6N2E2_3186
stress PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x N.D. -3.4 N.D. N.D. N.D.
stress RCH2 defined with Cobalt chloride 0.04 mM N.D. -2.6 N.D. N.D. N.D.
carbon source propionate (C) N.D. -2.4 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate A N.D. -2.4 N.D. N.D. N.D.
stress RCH2 defined with Zinc 0.032 mM N.D. -2.3 N.D. N.D. N.D.
carbon source propionate (C) N.D. -2.3 N.D. N.D. N.D.
stress RCH2 defined with Thallium(I) acetate 0.002 mg/ml N.D. -1.9 N.D. N.D. N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A N.D. -1.8 N.D. N.D. N.D.
carbon source RCH2_defined N.D. -1.7 N.D. N.D. N.D.
nitrogen source Cytidine (N) N.D. -1.6 N.D. N.D. N.D.
carbon source D-Lactate (C) N.D. -1.6 N.D. N.D. N.D.
nitrogen source Cytidine (N) N.D. -1.5 N.D. N.D. N.D.
carbon source D-Lactate (C) N.D. -1.4 N.D. N.D. N.D.
stress RCH2 defined with Cobalt chloride 0.04 mM N.D. -1.4 N.D. N.D. N.D.
nitrogen source Uridine (N) N.D. -1.3 N.D. N.D. N.D.
nitrogen source Gly-Glu (N) N.D. -1.3 N.D. N.D. N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C N.D. -1.3 N.D. N.D. N.D.
nitrogen source L-Aspartic Acid (N) N.D. -1.3 N.D. N.D. N.D.
stress Cadmium chloride hemipentahydrate 0.000015625 M N.D. -1.2 N.D. N.D. N.D.
stress RCH2 defined with Zinc 0.016 mM N.D. -1.2 N.D. N.D. N.D.
carbon source octanoate (C) N.D. -1.2 N.D. N.D. N.D.
stress Chlorite 0.00005 M N.D. -1.1 N.D. N.D. N.D.
denitrifying Nitrate reduction, replicate B N.D. -1.1 N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay N.D. +1.1 N.D. N.D. N.D.
resistance 20 mM putrescine N.D. +1.2 N.D. N.D. N.D.
stress DEANONOate 0.000625 M N.D. +1.2 N.D. N.D. N.D.
stress DEANONOate 0.000625 M N.D. +1.2 N.D. N.D. N.D.
stress tungstate 0.05 M N.D. +1.2 N.D. N.D. N.D.
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine N.D. +1.3 N.D. N.D. N.D.
stress Hydroxylamine 0.5 M N.D. +1.7 N.D. N.D. N.D.
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