Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2840 and Pf6N2E2_2841 are separated by 404 nucleotidesPf6N2E2_2841 and Pf6N2E2_2842 are separated by 169 nucleotidesPf6N2E2_2842 and Pf6N2E2_2843 are separated by 144 nucleotidesPf6N2E2_2843 and Pf6N2E2_2844 are separated by 94 nucleotides Pf6N2E2_2840 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,330,289 to 3,330,489 _2840 Pf6N2E2_2841 - Low-affinity inorganic phosphate transporter, at 3,330,894 to 3,332,330 _2841 Pf6N2E2_2842 - hypothetical protein, at 3,332,500 to 3,332,616 _2842 Pf6N2E2_2843 - FIG00959308: hypothetical protein, at 3,332,761 to 3,332,922 _2843 Pf6N2E2_2844 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 3,333,017 to 3,333,358 _2844
Group Condition Pf6N2E2_2840 Pf6N2E2_2841 Pf6N2E2_2842 Pf6N2E2_2843 Pf6N2E2_2844
carbon source octanoate (C) -0.7 N.D. -1.7 N.D. -0.0
carbon source Ying_Others16 rep B; time point 3 +0.0 N.D. -1.2 N.D. -0.8
carbon source Ying_all64 rep A; time point 3 -0.2 N.D. -0.3 N.D. -1.4
stress Fusidic 0.4 mg/ml -1.1 N.D. -0.0 N.D. -0.7
nitrogen source L-Serine (N) -0.2 N.D. -1.1 N.D. -0.5
stress Fusidic 0.3 mg/ml -0.9 N.D. -0.4 N.D. -0.5
carbon source Ying_OrganicAcid7 rep B; time point 3 -0.3 N.D. +0.1 N.D. -1.5
stress thiosulfate 0.25 M -0.3 N.D. -1.0 N.D. -0.4
stress RCH2 defined with Cobalt chloride 0.04 mM -0.6 N.D. -0.2 N.D. -0.8
nitrogen source nitrogen source nitrite 5mM -0.1 N.D. -0.5 N.D. -1.0
stress Bile salts 0.0125 M -0.3 N.D. -1.4 N.D. +0.1
carbon source Ying_sugar7 rep C; time point 1 +0.1 N.D. -1.1 N.D. -0.4
carbon source Ying_sugar7 rep B; time point 1 +0.2 N.D. -1.1 N.D. -0.4
carbon source Ying_Nucleobase14 rep A; time point 3 +0.2 N.D. -0.3 N.D. -1.2
carbon source Ying_sugar7 rep B; time point 2 -0.2 N.D. -1.3 N.D. +0.4
lb LB no stress 24 hours +0.4 N.D. -0.4 N.D. -0.9
carbon source Ying_AminoAcid20 rep C; time point 3 -0.2 N.D. +0.6 N.D. -1.3
stress Hydroxylamine 0.5 M +0.6 N.D. -0.7 N.D. -0.8
carbon source Ying_OrganicAcid7 rep C; time point 3 -0.1 N.D. +0.9 N.D. -1.3
carbon source Ying_all64 rep B; time point 3 -0.1 N.D. +1.1 N.D. -1.3
carbon source Ying_Nucleobase14 rep B; time point 3 +0.2 N.D. +0.6 N.D. -0.9
motility inner cut, LB soft agar motility assay +0.5 N.D. -0.6 N.D. +0.6
carbon source Ying_OrganicAcid7 rep A; time point 3 -0.0 N.D. +1.7 N.D. -0.9
denitrifying Nitrate reduction, replicate B -0.5 N.D. +1.2 N.D. +0.3
carbon source D-Xylose (C) +0.5 N.D. +1.1 N.D. +0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C -0.1 N.D. +1.4 N.D. +0.3
stress dimethyl sulfone 0.25 M +0.6 N.D. +0.6 N.D. +0.5
carbon source D-Fructose (C) +0.6 N.D. +1.1 N.D. +0.1
carbon source D-Galactose (C) +0.9 N.D. +1.3 N.D. +0.1
carbon source D-Galactose (C) +1.5 N.D. +0.7 N.D. +0.5
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