Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2598 and Pf6N2E2_2599 are separated by 11 nucleotidesPf6N2E2_2599 and Pf6N2E2_2600 are separated by 131 nucleotidesPf6N2E2_2600 and Pf6N2E2_2601 are separated by 82 nucleotidesPf6N2E2_2601 and Pf6N2E2_2602 are separated by 66 nucleotides Pf6N2E2_2598 - FIG00953167: hypothetical protein, at 3,056,691 to 3,056,987 _2598 Pf6N2E2_2599 - Flagellar biosynthesis protein FliS, at 3,056,999 to 3,057,394 _2599 Pf6N2E2_2600 - Flagellar hook-associated protein FliD, at 3,057,526 to 3,058,959 _2600 Pf6N2E2_2601 - Flagellin protein FlaG, at 3,059,042 to 3,059,413 _2601 Pf6N2E2_2602 - Flagellin protein FlaA, at 3,059,480 to 3,060,331 _2602
Group Condition Pf6N2E2_2598 Pf6N2E2_2599 Pf6N2E2_2600 Pf6N2E2_2601 Pf6N2E2_2602
motility inner cut, LB soft agar motility assay N.D. -3.0 -4.6 +1.9 -6.1
motility inner cut, LB soft agar motility assay N.D. -4.1 -3.8 +2.3 -5.7
motility outer cut, LB soft agar motility assay N.D. -4.1 -4.3 +2.1 -4.7
motility inner cut, LB soft agar motility assay N.D. -3.8 -4.3 +2.7 -3.9
motility outer cut, LB soft agar motility assay N.D. -3.3 -3.7 +1.8 -3.7
carbon source Ying_AminoAcid20 rep A; time point 2 N.D. -0.6 -1.0 +0.1 -0.7
carbon source Ying_OrganicAcid7 rep A; time point 2 N.D. -0.3 -0.5 -0.3 -1.0
stress Bile salts 0.0125 M N.D. -0.2 +0.1 -1.4 +0.5
nitrogen source L-Serine (N) N.D. +0.8 +0.5 -1.8 +0.6
carbon source Ying_AminoAcid20 rep A; time point 3 N.D. +0.5 +0.6 -0.3 +0.6
nitrogen source Glucuronamide (N) N.D. +0.7 +0.3 -0.2 +0.9
carbon source m-Inositol (C) N.D. +0.8 +0.6 -0.3 +0.8
carbon source L-Leucine (C) N.D. +0.5 +0.6 -0.1 +0.9
stress Nalidixic 0.008 mg/ml N.D. +0.8 +0.7 -0.3 +0.7
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine N.D. +0.7 +0.7 -0.2 +0.9
carbon source Ying_AminoAcid20 rep C; time point 3 N.D. +0.6 +0.7 +0.1 +0.6
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C N.D. +0.9 +0.3 +0.0 +0.9
nitrogen source Cytidine (N) N.D. +0.7 +0.8 -0.3 +0.9
stress Nalidixic 0.004 mg/ml N.D. +0.9 +0.7 +0.3 +0.2
carbon source L-Leucine (C) N.D. +0.7 +0.5 +0.3 +0.6
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. +0.7 +0.6 -0.1 +0.9
carbon source D-Xylose (C) N.D. +0.9 +0.6 -0.2 +0.8
carbon source 4-Hydroxybenzoic Acid (C) N.D. +0.8 +0.6 +0.1 +0.7
carbon source 4-Hydroxybenzoic Acid (C) N.D. +1.1 +0.6 -0.1 +0.9
nitrogen source Cytidine (N) N.D. +0.9 +0.8 -0.3 +0.9
carbon source Glycerol (C) N.D. +0.8 +0.7 +0.2 +1.0
stress Choline Lysine 20 mM N.D. +0.8 +0.9 +0.1 +0.9
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine N.D. +0.9 +0.9 +0.1 +1.2
carbon source D-Galactose (C) N.D. +1.3 +1.0 +0.1 +1.0
carbon source D-Galactose (C) N.D. +1.4 +0.9 +0.4 +0.8
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