Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2596 and Pf6N2E2_2597 are separated by 155 nucleotidesPf6N2E2_2597 and Pf6N2E2_2598 are separated by 169 nucleotidesPf6N2E2_2598 and Pf6N2E2_2599 are separated by 11 nucleotidesPf6N2E2_2599 and Pf6N2E2_2600 are separated by 131 nucleotides Pf6N2E2_2596 - Flagellar sensor histidine kinase FleS, at 3,053,727 to 3,054,890 _2596 Pf6N2E2_2597 - Flagellar regulatory protein FleQ, at 3,055,046 to 3,056,521 _2597 Pf6N2E2_2598 - FIG00953167: hypothetical protein, at 3,056,691 to 3,056,987 _2598 Pf6N2E2_2599 - Flagellar biosynthesis protein FliS, at 3,056,999 to 3,057,394 _2599 Pf6N2E2_2600 - Flagellar hook-associated protein FliD, at 3,057,526 to 3,058,959 _2600
Group Condition Pf6N2E2_2596 Pf6N2E2_2597 Pf6N2E2_2598 Pf6N2E2_2599 Pf6N2E2_2600
motility inner cut, LB soft agar motility assay -1.9 -6.2 N.D. -4.1 -3.8
motility inner cut, LB soft agar motility assay -1.8 -5.1 N.D. -3.8 -4.3
motility inner cut, LB soft agar motility assay -1.9 -5.5 N.D. -3.0 -4.6
motility outer cut, LB soft agar motility assay -1.3 -3.5 N.D. -4.1 -4.3
motility outer cut, LB soft agar motility assay -1.3 -3.4 N.D. -3.3 -3.7
stress Vancomycin 0.24 mg/ml -0.3 -1.8 N.D. -0.7 -0.5
carbon source Ying_OrganicAcid7 rep B; time point 3 +0.5 +2.3 N.D. -0.3 -0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B +0.5 +1.8 N.D. +0.6 -0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C +0.7 +1.7 N.D. +0.7 -0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A +0.7 +1.8 N.D. +0.5 -0.0
carbon source L-Phenylalanine (C) +0.4 +1.6 N.D. +0.7 +0.3
carbon source 4-Hydroxybenzoic Acid (C) +0.0 +1.3 N.D. +1.1 +0.6
carbon source Ying_AminoAcid20 rep B; time point 3 +0.6 +1.8 N.D. +0.1 +0.4
carbon source D-Xylose (C) +0.3 +1.2 N.D. +0.9 +0.6
denitrifying Nitrate reduction, replicate A +0.8 +1.6 N.D. +0.8 -0.1
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +0.3 +1.5 N.D. +0.7 +0.6
carbon source Ying_Others16 rep A; time point 2 +0.7 +1.9 N.D. +0.5 +0.1
nitrogen source Cytidine (N) +0.1 +1.7 N.D. +0.7 +0.8
carbon source Ying_Others16 rep C; time point 3 +0.7 +2.4 N.D. +0.2 +0.1
nitrogen source L-Serine (N) +0.2 +1.9 N.D. +0.8 +0.5
carbon source D-Galactose (C) -0.0 +1.2 N.D. +1.4 +0.9
carbon source D-Galactose (C) +0.1 +1.1 N.D. +1.3 +1.0
nitrogen source Cytidine (N) +0.2 +1.7 N.D. +0.9 +0.8
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B +0.8 +1.8 N.D. +1.0 +0.0
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C +0.9 +1.7 N.D. +0.9 +0.3
carbon source Ying_AminoAcid20 rep A; time point 3 +0.6 +2.2 N.D. +0.5 +0.6
carbon source Ying_Others16 rep A; time point 3 +0.7 +2.7 N.D. +0.3 +0.2
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +0.7 +2.1 N.D. +0.7 +0.7
carbon source Ying_AminoAcid20 rep C; time point 3 +0.7 +2.3 N.D. +0.6 +0.7
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +0.6 +2.0 N.D. +0.9 +0.9
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