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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
Pf6N2E2_2590 and Pf6N2E2_2591 overlap by 11 nucleotides
Pf6N2E2_2591 and Pf6N2E2_2592 are separated by 7 nucleotides
Pf6N2E2_2592 and Pf6N2E2_2593 overlap by 8 nucleotides
Pf6N2E2_2593 and Pf6N2E2_2594 are separated by 15 nucleotides
Pf6N2E2_2590 - Flagellum-specific ATP synthase FliI, at 3,046,787 to 3,048,145
_2590
Pf6N2E2_2591 - Flagellar assembly protein FliH, at 3,048,135 to 3,048,980
_2591
Pf6N2E2_2592 - Flagellar motor switch protein FliG, at 3,048,988 to 3,050,007
_2592
Pf6N2E2_2593 - Flagellar M-ring protein FliF, at 3,050,000 to 3,051,784
_2593
Pf6N2E2_2594 - Flagellar hook-basal body complex protein FliE, at 3,051,800 to 3,052,126
_2594
Group
Condition
Pf6N2E2
_2590
Pf6N2E2
_2591
Pf6N2E2
_2592
Pf6N2E2
_2593
Pf6N2E2
_2594
motility
inner cut, LB soft agar motility assay
-5.5
-5.8
-6.5
-4.4
-3.9
motility
inner cut, LB soft agar motility assay
-5.6
-4.4
-5.4
-5.0
-4.7
motility
outer cut, LB soft agar motility assay
-5.4
-4.5
-4.3
-6.2
-4.2
motility
outer cut, LB soft agar motility assay
-5.2
-5.9
-4.3
-3.6
-3.6
motility
inner cut, LB soft agar motility assay
-5.1
-3.5
-4.4
-4.5
-4.5
resistance
10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+1.1
+1.2
+1.2
+1.1
+1.2
stress
Rifampicin 0.003 mg/ml
+1.1
+0.9
+1.5
+1.1
+1.2
nitrogen source
nitrogen source nitrite 5mM
+1.0
+1.5
+1.1
+1.1
+1.2
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
+1.0
+1.1
+1.3
+1.3
+1.1
carbon source
octanoate (C)
+1.2
+1.4
+1.1
+1.0
+1.4
carbon source
L-Phenylalanine (C)
+1.2
+1.1
+1.2
+1.4
+1.3
denitrifying
Nitrate reduction, replicate B
+1.1
+1.0
+1.4
+1.5
+1.0
carbon source
L-Phenylalanine (C)
+1.2
+1.3
+1.2
+1.1
+1.3
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
+1.2
+1.2
+1.3
+1.4
+1.2
resistance
50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
+1.3
+1.2
+1.3
+1.3
+1.1
carbon source
Ying_Others16 rep B; time point 3
+1.5
+0.4
+1.3
+1.8
+1.4
carbon source
Ying_OrganicAcid7 rep A; time point 3
+1.6
+0.4
+1.4
+1.7
+1.5
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
+1.3
+1.3
+1.4
+1.3
+1.3
resistance
50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
+1.4
+1.4
+1.3
+1.4
+1.2
carbon source
Ying_AminoAcid20 rep B; time point 3
+1.6
+0.2
+1.7
+1.7
+1.7
nitrogen source
Cytidine (N)
+1.1
+1.3
+1.6
+1.2
+1.5
carbon source
Ying_OrganicAcid7 rep C; time point 3
+1.9
+0.1
+1.7
+1.7
+1.5
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
+1.5
+1.5
+1.4
+1.5
+1.2
carbon source
Ying_Others16 rep C; time point 3
+1.8
+0.1
+1.7
+1.9
+1.7
carbon source
Ying_OrganicAcid7 rep B; time point 3
+1.9
+0.4
+1.8
+1.7
+1.7
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
+1.5
+1.5
+1.7
+1.6
+1.4
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
+1.5
+1.7
+1.6
+1.6
+1.5
carbon source
Ying_Others16 rep A; time point 3
+1.9
+0.6
+1.9
+2.0
+1.7
carbon source
Ying_AminoAcid20 rep A; time point 3
+1.8
+0.5
+2.0
+1.9
+1.8
carbon source
Ying_AminoAcid20 rep C; time point 3
+2.1
+0.2
+2.1
+2.0
+2.0
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