Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2590 and Pf6N2E2_2591 overlap by 11 nucleotidesPf6N2E2_2591 and Pf6N2E2_2592 are separated by 7 nucleotidesPf6N2E2_2592 and Pf6N2E2_2593 overlap by 8 nucleotidesPf6N2E2_2593 and Pf6N2E2_2594 are separated by 15 nucleotides Pf6N2E2_2590 - Flagellum-specific ATP synthase FliI, at 3,046,787 to 3,048,145 _2590 Pf6N2E2_2591 - Flagellar assembly protein FliH, at 3,048,135 to 3,048,980 _2591 Pf6N2E2_2592 - Flagellar motor switch protein FliG, at 3,048,988 to 3,050,007 _2592 Pf6N2E2_2593 - Flagellar M-ring protein FliF, at 3,050,000 to 3,051,784 _2593 Pf6N2E2_2594 - Flagellar hook-basal body complex protein FliE, at 3,051,800 to 3,052,126 _2594
Group Condition Pf6N2E2_2590 Pf6N2E2_2591 Pf6N2E2_2592 Pf6N2E2_2593 Pf6N2E2_2594
motility inner cut, LB soft agar motility assay -5.5 -5.8 -6.5 -4.4 -3.9
motility inner cut, LB soft agar motility assay -5.6 -4.4 -5.4 -5.0 -4.7
motility outer cut, LB soft agar motility assay -5.4 -4.5 -4.3 -6.2 -4.2
motility outer cut, LB soft agar motility assay -5.2 -5.9 -4.3 -3.6 -3.6
motility inner cut, LB soft agar motility assay -5.1 -3.5 -4.4 -4.5 -4.5
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +1.1 +1.2 +1.2 +1.1 +1.2
stress Rifampicin 0.003 mg/ml +1.1 +0.9 +1.5 +1.1 +1.2
nitrogen source nitrogen source nitrite 5mM +1.0 +1.5 +1.1 +1.1 +1.2
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A +1.0 +1.1 +1.3 +1.3 +1.1
carbon source octanoate (C) +1.2 +1.4 +1.1 +1.0 +1.4
carbon source L-Phenylalanine (C) +1.2 +1.1 +1.2 +1.4 +1.3
denitrifying Nitrate reduction, replicate B +1.1 +1.0 +1.4 +1.5 +1.0
carbon source L-Phenylalanine (C) +1.2 +1.3 +1.2 +1.1 +1.3
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C +1.2 +1.2 +1.3 +1.4 +1.2
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +1.3 +1.2 +1.3 +1.3 +1.1
carbon source Ying_Others16 rep B; time point 3 +1.5 +0.4 +1.3 +1.8 +1.4
carbon source Ying_OrganicAcid7 rep A; time point 3 +1.6 +0.4 +1.4 +1.7 +1.5
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A +1.3 +1.3 +1.4 +1.3 +1.3
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +1.4 +1.4 +1.3 +1.4 +1.2
carbon source Ying_AminoAcid20 rep B; time point 3 +1.6 +0.2 +1.7 +1.7 +1.7
nitrogen source Cytidine (N) +1.1 +1.3 +1.6 +1.2 +1.5
carbon source Ying_OrganicAcid7 rep C; time point 3 +1.9 +0.1 +1.7 +1.7 +1.5
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B +1.5 +1.5 +1.4 +1.5 +1.2
carbon source Ying_Others16 rep C; time point 3 +1.8 +0.1 +1.7 +1.9 +1.7
carbon source Ying_OrganicAcid7 rep B; time point 3 +1.9 +0.4 +1.8 +1.7 +1.7
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B +1.5 +1.5 +1.7 +1.6 +1.4
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C +1.5 +1.7 +1.6 +1.6 +1.5
carbon source Ying_Others16 rep A; time point 3 +1.9 +0.6 +1.9 +2.0 +1.7
carbon source Ying_AminoAcid20 rep A; time point 3 +1.8 +0.5 +2.0 +1.9 +1.8
carbon source Ying_AminoAcid20 rep C; time point 3 +2.1 +0.2 +2.1 +2.0 +2.0
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