Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2525 and Pf6N2E2_2526 are separated by 4 nucleotidesPf6N2E2_2526 and Pf6N2E2_2527 are separated by 167 nucleotidesPf6N2E2_2527 and Pf6N2E2_2528 are separated by 353 nucleotidesPf6N2E2_2528 and Pf6N2E2_2529 are separated by 139 nucleotides Pf6N2E2_2525 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 2,982,418 to 2,983,116 _2525 Pf6N2E2_2526 - Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase, at 2,983,121 to 2,983,792 _2526 Pf6N2E2_2527 - Oxidoreductase, short chain dehydrogenase/reductase family, at 2,983,960 to 2,984,700 _2527 Pf6N2E2_2528 - GGDEF domain protein, at 2,985,054 to 2,985,980 _2528 Pf6N2E2_2529 - Pyrroloquinoline quinone (Coenzyme PQQ) biosynthesis protein C, at 2,986,120 to 2,986,923 _2529
Group Condition Pf6N2E2_2525 Pf6N2E2_2526 Pf6N2E2_2527 Pf6N2E2_2528 Pf6N2E2_2529
motility inner cut, LB soft agar motility assay N.D. -4.5 -4.8 +0.2 -0.0
carbon source Glycerol (C) N.D. -5.9 +0.2 +0.0 -0.3
carbon source Glycerol (C) N.D. -6.3 +0.8 +0.2 -0.5
motility inner cut, LB soft agar motility assay N.D. -2.3 -3.2 +0.1 -0.1
nitrogen source Cytidine (N) N.D. -0.6 -3.7 -0.0 -0.9
motility outer cut, LB soft agar motility assay N.D. -1.8 -3.6 +0.2 -0.0
stress Zinc chloride 0.0000015625 M N.D. -5.0 +0.3 -0.6 +0.1
motility outer cut, LB soft agar motility assay N.D. -1.9 -3.5 +0.3 +0.1
motility inner cut, LB soft agar motility assay N.D. -1.7 -2.9 -0.1 +0.1
nitrogen source Cytidine (N) N.D. -0.8 -2.9 +0.1 -0.7
stress 5-Hydroxymethylfurfural 10 mM N.D. -2.4 -1.7 +0.1 +0.0
carbon source D-Alanine (C) N.D. -2.1 -1.8 +0.2 -0.4
carbon source D-Alanine (C) N.D. -1.4 -2.5 +0.0 -0.0
nitrogen source Cytosine (N) N.D. -0.3 -3.2 +0.2 -0.4
nitrogen source L-Serine (N) N.D. -3.1 -0.3 +0.1 -0.4
carbon source Carnitine Hydrochloride (C) N.D. -0.5 -3.3 +0.2 -0.1
nitrogen source L-Isoleucine (N) N.D. -2.1 -0.9 +0.0 -0.5
stress Phosphomycin 0.004 mg/ml N.D. -2.9 -0.4 +0.6 -0.2
vitamin No vitamins t2 N.D. -0.8 -2.5 +0.3 +0.4
carbon source D-Galactose (C) N.D. -1.3 +3.3 -0.2 -0.5
carbon source D-Galactose (C) N.D. -1.1 +3.6 -0.1 -0.6
carbon source m-Inositol (C) N.D. +0.4 +2.6 -0.4 -0.4
stress arsenate 0.0005 M N.D. +3.1 -0.7 +0.2 +0.1
carbon source D-Fructose (C) N.D. -0.4 +3.0 +0.8 -0.4
stress arsenate 0.0005 M N.D. +3.7 -0.7 +0.1 -0.0
stress Bile salts 0.0125 M N.D. +1.5 +2.6 -0.3 +0.0
stress DEANONOate 0.000625 M N.D. +1.8 +3.2 -0.1 -0.2
stress DEANONOate 0.000625 M N.D. +1.8 +2.9 +0.2 -0.1
stress Rifampicin 0.003 mg/ml N.D. +3.2 +1.1 +0.7 -0.1
stress Hydroxylamine 0.5 M N.D. +2.5 +3.4 +0.1 +0.1
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