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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
Pf6N2E2_2424 and Pf6N2E2_2425 are separated by 75 nucleotides
Pf6N2E2_2425 and Pf6N2E2_2426 are separated by 78 nucleotides
Pf6N2E2_2426 and Pf6N2E2_2427 are separated by 72 nucleotides
Pf6N2E2_2427 and Pf6N2E2_2428 are separated by 92 nucleotides
Pf6N2E2_2424 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 2,874,663 to 2,875,265
_2424
Pf6N2E2_2425 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,875,341 to 2,875,562
_2425
Pf6N2E2_2426 - Similar to ribosomal large subunit pseudouridine synthase A, at 2,875,641 to 2,876,531
_2426
Pf6N2E2_2427 - DNA damage response gene with a putative DNA binding domain and often regulated by LexA (PA0922-like)
(from data)
, at 2,876,604 to 2,876,924
_2427
Pf6N2E2_2428 - Thiol-disulfide isomerase and thioredoxins, at 2,877,017 to 2,877,253
_2428
Group
Condition
Pf6N2E2
_2424
Pf6N2E2
_2425
Pf6N2E2
_2426
Pf6N2E2
_2427
Pf6N2E2
_2428
motility
inner cut, LB soft agar motility assay
-1.6
+0.5
+0.6
-0.0
-4.5
stress
4-Hydroxyacetophenone 0.00025 M
-0.3
-0.2
-0.2
-0.3
-4.0
stress
Chloride 600 mM
+0.1
-2.7
-0.5
-0.3
-0.6
nitrogen source
nitrogen source nitrate 5mM
-1.5
-0.7
-1.0
+0.1
-0.5
stress
phosphite 0.25 M
-0.2
-3.0
+0.0
-0.1
-0.1
motility
outer cut, LB soft agar motility assay
-1.5
-0.1
+0.1
+0.5
-2.3
stress
Magnesium chloride 0.125 M
-0.9
-1.6
-0.2
+0.2
-0.8
lb
LB no stress 48 hours
-1.3
-1.0
-0.1
+0.1
-0.8
motility
outer cut, LB soft agar motility assay
-1.1
+0.3
+0.1
+0.1
-2.4
stress
deoxycholate 0.0125 M
-0.0
-3.5
-0.7
+0.7
+0.8
stress
Cisplatin 0.008 mg/ml
+0.1
+0.1
+0.1
-1.7
-1.4
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
-3.5
-0.3
+0.7
+0.0
+0.4
stress
RCH2 defined with Cobalt chloride 0.04 mM
-1.1
-2.4
+1.6
-0.2
-0.4
nitrogen source
L-Serine (N)
-0.9
-0.3
+0.5
+0.3
-1.6
stress
RCH2 defined with Cobalt chloride 0.04 mM
-0.6
-2.0
+1.6
-0.6
-0.2
stress
Bile salts 0.0125 M
+0.3
-3.2
+0.2
-0.0
+1.1
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
-2.4
-0.4
+0.4
+0.0
+0.7
nitrogen source
nitrogen source nitrate 5mM
-0.7
+1.0
-1.0
-0.4
+0.1
denitrifying
Nitrate reduction, replicate B
-1.9
-0.3
+0.5
+0.1
+0.7
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
-2.3
-0.3
+0.7
+0.1
+0.9
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
-2.1
-0.0
+0.6
+0.2
+0.5
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
-2.1
-0.3
+0.6
+0.0
+1.1
denitrifying
Nitrate reduction, replicate A
-1.9
-0.2
+0.7
+0.3
+0.6
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
-2.1
-0.2
+0.6
+0.1
+1.0
stress
Phosphomycin 0.004 mg/ml
-0.8
+1.0
-0.8
+0.5
-0.3
stress
DEANONOate 0.000625 M
-1.2
-0.7
-0.0
-0.3
+3.6
carbon source
octanoate (C)
-0.5
+0.6
+0.6
-0.3
+1.5
carbon source
D-Galactose (C)
-0.3
-0.2
+0.1
+0.4
+2.3
stress
DEANONOate 0.000625 M
-0.2
+0.4
+0.1
-0.0
+3.3
stress
Hydroxylamine 0.5 M
+0.5
-0.4
-0.4
-0.2
+4.9
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