Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2424 and Pf6N2E2_2425 are separated by 75 nucleotidesPf6N2E2_2425 and Pf6N2E2_2426 are separated by 78 nucleotidesPf6N2E2_2426 and Pf6N2E2_2427 are separated by 72 nucleotidesPf6N2E2_2427 and Pf6N2E2_2428 are separated by 92 nucleotides Pf6N2E2_2424 - Molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 2,874,663 to 2,875,265 _2424 Pf6N2E2_2425 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 2,875,341 to 2,875,562 _2425 Pf6N2E2_2426 - Similar to ribosomal large subunit pseudouridine synthase A, at 2,875,641 to 2,876,531 _2426 Pf6N2E2_2427 - DNA damage response gene with a putative DNA binding domain and often regulated by LexA (PA0922-like) (from data), at 2,876,604 to 2,876,924 _2427 Pf6N2E2_2428 - Thiol-disulfide isomerase and thioredoxins, at 2,877,017 to 2,877,253 _2428
Group Condition Pf6N2E2_2424 Pf6N2E2_2425 Pf6N2E2_2426 Pf6N2E2_2427 Pf6N2E2_2428
motility inner cut, LB soft agar motility assay -1.6 +0.5 +0.6 -0.0 -4.5
stress 4-Hydroxyacetophenone 0.00025 M -0.3 -0.2 -0.2 -0.3 -4.0
stress Chloride 600 mM +0.1 -2.7 -0.5 -0.3 -0.6
nitrogen source nitrogen source nitrate 5mM -1.5 -0.7 -1.0 +0.1 -0.5
stress phosphite 0.25 M -0.2 -3.0 +0.0 -0.1 -0.1
motility outer cut, LB soft agar motility assay -1.5 -0.1 +0.1 +0.5 -2.3
stress Magnesium chloride 0.125 M -0.9 -1.6 -0.2 +0.2 -0.8
lb LB no stress 48 hours -1.3 -1.0 -0.1 +0.1 -0.8
motility outer cut, LB soft agar motility assay -1.1 +0.3 +0.1 +0.1 -2.4
stress deoxycholate 0.0125 M -0.0 -3.5 -0.7 +0.7 +0.8
stress Cisplatin 0.008 mg/ml +0.1 +0.1 +0.1 -1.7 -1.4
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B -3.5 -0.3 +0.7 +0.0 +0.4
stress RCH2 defined with Cobalt chloride 0.04 mM -1.1 -2.4 +1.6 -0.2 -0.4
nitrogen source L-Serine (N) -0.9 -0.3 +0.5 +0.3 -1.6
stress RCH2 defined with Cobalt chloride 0.04 mM -0.6 -2.0 +1.6 -0.6 -0.2
stress Bile salts 0.0125 M +0.3 -3.2 +0.2 -0.0 +1.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A -2.4 -0.4 +0.4 +0.0 +0.7
nitrogen source nitrogen source nitrate 5mM -0.7 +1.0 -1.0 -0.4 +0.1
denitrifying Nitrate reduction, replicate B -1.9 -0.3 +0.5 +0.1 +0.7
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B -2.3 -0.3 +0.7 +0.1 +0.9
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C -2.1 -0.0 +0.6 +0.2 +0.5
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A -2.1 -0.3 +0.6 +0.0 +1.1
denitrifying Nitrate reduction, replicate A -1.9 -0.2 +0.7 +0.3 +0.6
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C -2.1 -0.2 +0.6 +0.1 +1.0
stress Phosphomycin 0.004 mg/ml -0.8 +1.0 -0.8 +0.5 -0.3
stress DEANONOate 0.000625 M -1.2 -0.7 -0.0 -0.3 +3.6
carbon source octanoate (C) -0.5 +0.6 +0.6 -0.3 +1.5
carbon source D-Galactose (C) -0.3 -0.2 +0.1 +0.4 +2.3
stress DEANONOate 0.000625 M -0.2 +0.4 +0.1 -0.0 +3.3
stress Hydroxylamine 0.5 M +0.5 -0.4 -0.4 -0.2 +4.9
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