Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2298 and Pf6N2E2_2299 overlap by 4 nucleotidesPf6N2E2_2299 and Pf6N2E2_2300 are separated by 187 nucleotidesPf6N2E2_2300 and Pf6N2E2_2301 are separated by 172 nucleotidesPf6N2E2_2301 and Pf6N2E2_2302 are separated by 361 nucleotides Pf6N2E2_2298 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 2,721,089 to 2,722,423 _2298 Pf6N2E2_2299 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusA, at 2,722,420 to 2,722,671 _2299 Pf6N2E2_2300 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 2,722,859 to 2,723,932 _2300 Pf6N2E2_2301 - Aconitate hydratase (EC 4.2.1.3), at 2,724,105 to 2,726,846 _2301 Pf6N2E2_2302 - CAAX amino terminal protease family protein, at 2,727,208 to 2,727,996 _2302
Group Condition Pf6N2E2_2298 Pf6N2E2_2299 Pf6N2E2_2300 Pf6N2E2_2301 Pf6N2E2_2302
vitamin With vitamins t1 +0.1 N.D. -1.8 -0.3 -0.1
carbon source Ying_AminoAcid20 rep A; time point 2 -0.0 N.D. -1.4 +0.1 -0.3
carbon source Ying_OrganicAcid7 rep B; time point 2 +0.0 N.D. -1.3 -0.1 -0.2
stress RCH2 defined with Cobalt chloride 0.04 mM -0.6 N.D. -0.8 -0.2 +0.1
carbon source Ying_OrganicAcid7 rep A; time point 2 +0.1 N.D. -1.3 -0.1 -0.1
carbon source Carbon source D,L-Lactate 10 mM -0.0 N.D. -1.5 -0.0 +0.2
stress RCH2 defined with Cobalt chloride 0.04 mM -0.5 N.D. -1.2 -0.1 +0.5
carbon source Ying_AminoAcid20 rep B; time point 3 +0.0 N.D. -1.3 -0.0 -0.0
carbon source Ying_Nucleobase14 rep B; time point 1 +0.1 N.D. -1.2 +0.1 -0.1
stress Chloride 0.5 M +0.2 N.D. -1.5 +0.2 +0.1
carbon source Ying_OrganicAcid7 rep C; time point 2 +0.1 N.D. -1.2 +0.0 +0.1
carbon source Carbon source Sucrose 5 mM -0.1 N.D. -1.0 -0.0 +0.3
stress rubidium chloride 0.25 M +0.1 N.D. -1.0 +0.0 +0.3
carbon source D-Glucosamine Hydrochloride (C) +0.1 N.D. -0.6 -0.3 +0.4
stress Nickel (II) chloride 1.5 mM +0.1 N.D. -1.1 +0.2 +0.5
lb LB no stress 48 hours +0.1 N.D. -0.9 +0.2 +0.4
stress periodate 0.00125 M -0.2 N.D. -0.7 +0.2 +0.5
stress sodium iodate 0.005 M +0.4 N.D. -0.9 +0.2 +0.2
stress Cobalt chloride 0.6 mM +0.7 N.D. -0.3 +0.1 -0.2
stress Nickel (II) chloride 0.0025 M +0.6 N.D. -0.5 -0.1 +0.5
stress Zinc chloride 0.0000015625 M +0.2 N.D. +0.9 -0.1 -0.1
stress Cobalt chloride 0.6 mM +0.9 N.D. -0.3 +0.1 +0.2
carbon source propionate (C) +0.3 N.D. +0.6 -0.2 +0.4
stress 4-Hydroxyacetophenone 0.00025 M +0.1 N.D. +0.3 +0.2 +0.8
motility outer cut, LB soft agar motility assay -0.2 N.D. -0.1 +0.2 +1.5
carbon source L-Alanine (C) +0.5 N.D. +0.5 +0.1 +0.3
motility outer cut, LB soft agar motility assay +0.1 N.D. +0.2 +0.1 +1.2
motility inner cut, LB soft agar motility assay -0.0 N.D. -0.3 +0.2 +2.0
motility inner cut, LB soft agar motility assay +0.3 N.D. +0.7 +0.1 +1.6
motility inner cut, LB soft agar motility assay +0.1 N.D. +0.1 +0.3 +2.2
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Pf6N2E2_2298
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