Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2273 and Pf6N2E2_2274 are separated by 42 nucleotidesPf6N2E2_2274 and Pf6N2E2_2275 are separated by 346 nucleotidesPf6N2E2_2275 and Pf6N2E2_2276 are separated by 130 nucleotidesPf6N2E2_2276 and Pf6N2E2_2277 are separated by 99 nucleotides Pf6N2E2_2273 - Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46), at 2,687,072 to 2,687,794 _2273 Pf6N2E2_2274 - Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1), at 2,687,837 to 2,689,231 _2274 Pf6N2E2_2275 - Thiamin biosynthesis lipoprotein ApbE, at 2,689,578 to 2,690,567 _2275 Pf6N2E2_2276 - Permeases of the major facilitator superfamily, at 2,690,698 to 2,691,996 _2276 Pf6N2E2_2277 - NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13), at 2,692,096 to 2,693,481 _2277
Group Condition Pf6N2E2_2273 Pf6N2E2_2274 Pf6N2E2_2275 Pf6N2E2_2276 Pf6N2E2_2277
motility inner cut, LB soft agar motility assay -0.9 -2.9 -0.3 -0.0 -3.4
stress acetate 80 mM -0.1 -3.8 +0.0 -0.2 -3.2
carbon source Ying_OrganicAcid7 rep C; time point 3 -1.1 -0.1 -0.1 -0.5 -5.4
carbon source octanoate (C) -0.2 -4.1 +0.4 -0.2 -3.1
carbon source Ying_OrganicAcid7 rep A; time point 3 -0.8 -0.2 -0.0 -0.6 -5.4
motility outer cut, LB soft agar motility assay -0.5 -3.2 +0.1 -0.2 -3.1
carbon source Ying_OrganicAcid7 rep B; time point 3 -0.7 -0.2 -0.2 -0.2 -5.4
carbon source L-Alanine (C) +0.0 -0.9 +0.1 -0.2 -5.5
carbon source Ying_AminoAcid20 rep C; time point 3 -0.3 -0.3 +0.0 -0.6 -5.3
carbon source Trisodium citrate (C) -0.1 -1.2 +0.0 +0.1 -5.1
motility inner cut, LB soft agar motility assay -0.0 -2.5 -0.2 +0.2 -3.8
carbon source Ying_AminoAcid20 rep A; time point 3 -0.1 -0.3 -0.0 -0.1 -5.3
carbon source Ying_AminoAcid20 rep C; time point 2 -0.2 -0.4 +0.0 -0.2 -4.9
stress Cephalothin 4 mg/ml -0.5 -3.7 +0.1 +0.2 -1.7
carbon source D-Alanine (C) +0.7 -0.7 +0.1 -0.2 -5.6
carbon source propionate (C) -0.3 -2.8 +0.4 -0.3 -2.6
carbon source octanoate (C) -0.5 -4.1 +0.5 +0.4 -1.8
carbon source Putrescine (C) +0.1 -0.1 +0.1 -0.1 -5.6
nitrogen source Putrescine (N) -0.0 -4.7 -0.1 +0.2 -0.8
carbon source L-Leucine (C) -0.5 -1.3 -0.1 +0.1 -3.6
carbon source Ying_AminoAcid20 rep B; time point 3 -0.2 +0.1 +0.0 +0.0 -5.3
carbon source pyruvate (C) +0.1 +0.2 +0.1 -0.2 -5.5
carbon source propionate (C) -0.2 -2.8 +0.1 +0.2 -2.5
carbon source a-Ketoglutaric (C) -0.3 -0.2 +0.3 -0.1 -4.8
carbon source L-Histidine (C) -0.4 -0.8 +0.1 +0.3 -4.0
carbon source RCH2_defined +0.3 -2.5 +0.2 +0.1 -2.7
stress Nickel (II) chloride 0.0025 M +0.3 -2.8 +0.3 +0.1 -2.0
carbon source L-Lactate (C) +0.2 +0.5 +0.1 -0.2 -4.5
carbon source D-Lactate (C) +0.2 +0.6 +0.1 -0.2 -4.5
stress copper (II) chloride 3 mM -0.1 -4.5 +0.1 +0.2 +1.8
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