Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_2265 and Pf6N2E2_2266 are separated by 157 nucleotidesPf6N2E2_2266 and Pf6N2E2_2267 overlap by 4 nucleotidesPf6N2E2_2267 and Pf6N2E2_2268 are separated by 698 nucleotidesPf6N2E2_2268 and Pf6N2E2_2269 are separated by 63 nucleotides Pf6N2E2_2265 - Copper tolerance protein, at 2,679,698 to 2,680,243 _2265 Pf6N2E2_2266 - Copper-sensing two-component system response regulator CusR, at 2,680,401 to 2,681,081 _2266 Pf6N2E2_2267 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 2,681,078 to 2,682,436 _2267 Pf6N2E2_2268 - Lipoprotein releasing system transmembrane protein LolE, at 2,683,135 to 2,684,379 _2268 Pf6N2E2_2269 - Lipoprotein releasing system ATP-binding protein LolD, at 2,684,443 to 2,685,141 _2269
Group Condition Pf6N2E2_2265 Pf6N2E2_2266 Pf6N2E2_2267 Pf6N2E2_2268 Pf6N2E2_2269
stress copper (II) chloride 3 mM -1.5 -4.5 -6.3 N.D. N.D.
stress RCH2 defined with copper (II) chloride 0.03 mM -1.0 -1.1 -2.7 N.D. N.D.
stress RCH2 defined with copper (II) chloride 0.03 mM -1.5 -0.4 -2.3 N.D. N.D.
nitrogen source L-Serine (N) +0.6 -0.7 -2.6 N.D. N.D.
nitrogen source Glycine (N) -0.6 -0.5 -1.0 N.D. N.D.
stress RCH2 defined with Nickel (II) chloride 0.04 mM -0.0 -0.5 -1.3 N.D. N.D.
stress Cobalt chloride 0.6 mM -0.4 -0.7 -0.6 N.D. N.D.
nitrogen source nitrogen source nitrite 0.5mM -0.8 -0.7 -0.1 N.D. N.D.
stress RCH2 defined with Nickel (II) chloride 0.04 mM -0.1 -0.6 -0.9 N.D. N.D.
stress RCH2 defined with Cobalt chloride 0.04 mM -1.7 -0.3 +0.4 N.D. N.D.
motility inner cut, LB soft agar motility assay +0.6 -1.6 -0.4 N.D. N.D.
nitrogen source Glucuronamide (N) -0.3 +0.4 -1.4 N.D. N.D.
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C -0.7 -0.5 +0.5 N.D. N.D.
lb LB no stress 48 hours -0.3 -1.1 +0.7 N.D. N.D.
carbon source Ying_sugar7 rep B; time point 2 -0.6 -0.7 +0.7 N.D. N.D.
stress Hydrochloric Acid 0.005 M +0.1 -1.1 +0.5 N.D. N.D.
stress Chloride 600 mM +0.5 -1.1 +0.2 N.D. N.D.
carbon source Ying_sugar7 rep B; time point 1 -0.6 -0.5 +0.6 N.D. N.D.
stress Chlorite 0.00005 M +0.1 -1.3 +0.7 N.D. N.D.
lb LB no stress 48 hours -0.6 -0.4 +0.9 N.D. N.D.
carbon source D-Fructose (C) +0.8 -0.6 -0.2 N.D. N.D.
stress thiosulfate 0.25 M +0.6 -0.9 +0.4 N.D. N.D.
stress Cisplatin 0.008 mg/ml +0.6 -0.7 +0.3 N.D. N.D.
stress dimethyl sulfone 0.25 M +0.6 -0.9 +0.5 N.D. N.D.
stress Bile salts 0.0125 M -0.9 +1.0 +0.2 N.D. N.D.
nitrogen source L-Glutamic (N) +0.4 +0.8 -0.6 N.D. N.D.
stress pyrophosphate 0.0025 M +0.7 -0.5 +0.7 N.D. N.D.
carbon source D-Galactose (C) +0.9 +0.5 +0.5 N.D. N.D.
stress Vanillyl Alcohol 0.005 M +0.5 +0.6 +0.8 N.D. N.D.
stress deoxycholate 0.0125 M +0.7 +0.6 +0.9 N.D. N.D.
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