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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_2265 and Pf6N2E2_2266 are separated by 157 nucleotides
Pf6N2E2_2266 and Pf6N2E2_2267 overlap by 4 nucleotides
Pf6N2E2_2267 and Pf6N2E2_2268 are separated by 698 nucleotides
Pf6N2E2_2268 and Pf6N2E2_2269 are separated by 63 nucleotides
Pf6N2E2_2265 - Copper tolerance protein, at 2,679,698 to 2,680,243
_2265
Pf6N2E2_2266 - Copper-sensing two-component system response regulator CusR, at 2,680,401 to 2,681,081
_2266
Pf6N2E2_2267 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 2,681,078 to 2,682,436
_2267
Pf6N2E2_2268 - Lipoprotein releasing system transmembrane protein LolE, at 2,683,135 to 2,684,379
_2268
Pf6N2E2_2269 - Lipoprotein releasing system ATP-binding protein LolD, at 2,684,443 to 2,685,141
_2269
Group
Condition
Pf6N2E2
_2265
Pf6N2E2
_2266
Pf6N2E2
_2267
Pf6N2E2
_2268
Pf6N2E2
_2269
stress
copper (II) chloride 3 mM
-1.5
-4.5
-6.3
N.D.
N.D.
stress
RCH2 defined with copper (II) chloride 0.03 mM
-1.0
-1.1
-2.7
N.D.
N.D.
stress
RCH2 defined with copper (II) chloride 0.03 mM
-1.5
-0.4
-2.3
N.D.
N.D.
nitrogen source
L-Serine (N)
+0.6
-0.7
-2.6
N.D.
N.D.
nitrogen source
Glycine (N)
-0.6
-0.5
-1.0
N.D.
N.D.
stress
RCH2 defined with Nickel (II) chloride 0.04 mM
-0.0
-0.5
-1.3
N.D.
N.D.
stress
Cobalt chloride 0.6 mM
-0.4
-0.7
-0.6
N.D.
N.D.
nitrogen source
nitrogen source nitrite 0.5mM
-0.8
-0.7
-0.1
N.D.
N.D.
stress
RCH2 defined with Nickel (II) chloride 0.04 mM
-0.1
-0.6
-0.9
N.D.
N.D.
stress
RCH2 defined with Cobalt chloride 0.04 mM
-1.7
-0.3
+0.4
N.D.
N.D.
motility
inner cut, LB soft agar motility assay
+0.6
-1.6
-0.4
N.D.
N.D.
nitrogen source
Glucuronamide (N)
-0.3
+0.4
-1.4
N.D.
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate C
-0.7
-0.5
+0.5
N.D.
N.D.
lb
LB no stress 48 hours
-0.3
-1.1
+0.7
N.D.
N.D.
carbon source
Ying_sugar7 rep B; time point 2
-0.6
-0.7
+0.7
N.D.
N.D.
stress
Hydrochloric Acid 0.005 M
+0.1
-1.1
+0.5
N.D.
N.D.
stress
Chloride 600 mM
+0.5
-1.1
+0.2
N.D.
N.D.
carbon source
Ying_sugar7 rep B; time point 1
-0.6
-0.5
+0.6
N.D.
N.D.
stress
Chlorite 0.00005 M
+0.1
-1.3
+0.7
N.D.
N.D.
lb
LB no stress 48 hours
-0.6
-0.4
+0.9
N.D.
N.D.
carbon source
D-Fructose (C)
+0.8
-0.6
-0.2
N.D.
N.D.
stress
thiosulfate 0.25 M
+0.6
-0.9
+0.4
N.D.
N.D.
stress
Cisplatin 0.008 mg/ml
+0.6
-0.7
+0.3
N.D.
N.D.
stress
dimethyl sulfone 0.25 M
+0.6
-0.9
+0.5
N.D.
N.D.
stress
Bile salts 0.0125 M
-0.9
+1.0
+0.2
N.D.
N.D.
nitrogen source
L-Glutamic (N)
+0.4
+0.8
-0.6
N.D.
N.D.
stress
pyrophosphate 0.0025 M
+0.7
-0.5
+0.7
N.D.
N.D.
carbon source
D-Galactose (C)
+0.9
+0.5
+0.5
N.D.
N.D.
stress
Vanillyl Alcohol 0.005 M
+0.5
+0.6
+0.8
N.D.
N.D.
stress
deoxycholate 0.0125 M
+0.7
+0.6
+0.9
N.D.
N.D.
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