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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_2199 and Pf6N2E2_2200 are separated by 168 nucleotides
Pf6N2E2_2200 and Pf6N2E2_2201 overlap by 4 nucleotides
Pf6N2E2_2201 and Pf6N2E2_2202 are separated by 24 nucleotides
Pf6N2E2_2202 and Pf6N2E2_2203 are separated by 327 nucleotides
Pf6N2E2_2199 - Catalase (EC 1.11.1.6), at 2,605,658 to 2,606,740
_2199
Pf6N2E2_2200 - RNA polymerase sigma-54 factor RpoN, at 2,606,909 to 2,607,415
_2200
Pf6N2E2_2201 - Transmembrane regulator protein PrtR, at 2,607,412 to 2,608,164
_2201
Pf6N2E2_2202 - Transcriptional regulator, IclR family, at 2,608,189 to 2,608,494
_2202
Pf6N2E2_2203 - Mobile element protein, at 2,608,822 to 2,610,090
_2203
Group
Condition
Pf6N2E2
_2199
Pf6N2E2
_2200
Pf6N2E2
_2201
Pf6N2E2
_2202
Pf6N2E2
_2203
stress
thiosulfate 0.25 M
+0.0
-1.2
-3.1
N.D.
N.D.
stress
Hydroxylamine 0.5 M
+0.2
-3.1
-0.9
N.D.
N.D.
nitrogen source
nitrogen source nitrite 1mM
+0.0
-0.8
-2.8
N.D.
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
-0.0
-1.9
-1.6
N.D.
N.D.
stress
tungstate 0.05 M
-0.2
-2.1
-1.1
N.D.
N.D.
stress
dimethyl sulfone 0.25 M
+0.1
+0.5
-3.8
N.D.
N.D.
stress
4-Hydroxyacetophenone 0.00025 M
-0.0
-1.4
-1.8
N.D.
N.D.
stress
4-Hydroxyacetophenone 0.00025 M
+0.1
-0.3
-2.9
N.D.
N.D.
motility
outer cut, LB soft agar motility assay
+0.0
+0.6
-3.6
N.D.
N.D.
stress
molybdate 0.25 M
+0.2
-1.2
-1.8
N.D.
N.D.
stress
Fluorophosphate 0.125 M
+0.3
+0.1
-3.1
N.D.
N.D.
nitrogen source
nitrogen source nitrite 5mM
+0.0
+0.5
-3.1
N.D.
N.D.
nitrogen source
Glucuronamide (N)
-0.1
-2.9
+0.7
N.D.
N.D.
motility
inner cut, LB soft agar motility assay
+0.1
+0.3
-2.6
N.D.
N.D.
stress
DEANONOate 0.000625 M
+0.5
-3.2
+1.0
N.D.
N.D.
stress
Bile salts 0.0125 M
+0.5
-2.3
+0.1
N.D.
N.D.
nitrogen source
nitrogen source nitrite 0.5mM
-0.0
+0.9
-2.6
N.D.
N.D.
nitrogen source
nitrogen source nitrate 5mM
+0.1
+0.6
-2.3
N.D.
N.D.
stress
Vanillyl Alcohol 0.005 M
+0.1
+1.0
-2.5
N.D.
N.D.
stress
Chlorite 0.00005 M
+0.0
+0.8
-2.2
N.D.
N.D.
motility
inner cut, LB soft agar motility assay
+0.4
+0.5
-2.3
N.D.
N.D.
nitrogen source
L-Serine (N)
+0.3
-2.2
+0.9
N.D.
N.D.
stress
Magnesium chloride 0.125 M
+0.1
-1.9
+0.9
N.D.
N.D.
stress
molybdate 0.25 M
+0.2
+1.1
-1.7
N.D.
N.D.
carbon source
D-Fructose (C)
-0.0
-2.5
+2.9
N.D.
N.D.
carbon source
D-Galactose (C)
-0.2
-1.6
+2.3
N.D.
N.D.
carbon source
D-Galactose (C)
-0.0
-1.4
+2.3
N.D.
N.D.
carbon source
D-Glucosamine Hydrochloride (C)
-0.1
-0.5
+2.4
N.D.
N.D.
carbon source
m-Inositol (C)
-0.2
-0.3
+2.5
N.D.
N.D.
carbon source
D-Glucosamine Hydrochloride (C)
+0.2
+0.3
+3.1
N.D.
N.D.
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