Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_1833 and Pf6N2E2_1834 are separated by 2 nucleotidesPf6N2E2_1834 and Pf6N2E2_1835 overlap by 4 nucleotidesPf6N2E2_1835 and Pf6N2E2_1836 are separated by 170 nucleotidesPf6N2E2_1836 and Pf6N2E2_1837 are separated by 6 nucleotides Pf6N2E2_1833 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 2,171,151 to 2,172,668 _1833 Pf6N2E2_1834 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,172,671 to 2,173,441 _1834 Pf6N2E2_1835 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,173,438 to 2,174,049 _1835 Pf6N2E2_1836 - Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16), at 2,174,220 to 2,175,422 _1836 Pf6N2E2_1837 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 2,175,429 to 2,176,907 _1837
Group Condition Pf6N2E2_1833 Pf6N2E2_1834 Pf6N2E2_1835 Pf6N2E2_1836 Pf6N2E2_1837
carbon source Ying_Nucleobase14 rep B; time point 2 -0.3 -1.6 -0.8 -0.4 -0.0
carbon source Ying_OrganicAcid7 rep A; time point 2 -0.1 -2.3 -0.3 -0.1 -0.0
nitrogen source nitrogen source nitrite 1mM -0.0 -3.1 +0.3 -0.0 +0.3
carbon source Ying_sugar7 rep B; time point 2 -0.2 -1.8 -0.7 +0.2 +0.2
nitrogen source nitrogen source nitrate 20mM +0.1 -2.8 +0.2 +0.6 -0.3
stress Chloride 600 mM +0.2 -2.0 -0.0 -0.0 -0.2
motility inner cut, LB soft agar motility assay +0.3 -2.3 -0.4 +0.4 -0.1
motility outer cut, LB soft agar motility assay -0.1 -0.7 -0.7 -0.7 +0.3
carbon source D-Trehalose (C) -1.1 -0.5 -0.1 +0.3 -0.4
stress Cobalt chloride 0.6 mM +0.1 -1.5 -0.1 +0.1 -0.4
stress bromate 0.05 M +0.1 -1.4 -0.7 -0.0 +0.3
nitrogen source L-Glutamine (N) -0.8 -0.6 -0.4 +0.0 +0.4
carbon source Ying_Nucleobase14 rep C; time point 1 -0.1 -1.6 -0.2 -0.0 +0.5
stress Bile salts 0.0125 M -1.4 -1.7 +0.8 +0.7 +0.3
stress Nickel (II) chloride 1 mM +0.5 -1.1 -0.5 -0.1 -0.1
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B -0.4 -1.0 +0.5 -0.4 -0.0
stress dimethyl sulfone 0.25 M -0.2 -2.0 +0.2 +0.2 +0.6
stress Chlorite 0.00005 M +0.0 -1.7 +0.0 +0.5 +0.1
lb LB no stress 24 hours +0.2 -1.0 -0.5 -0.1 +0.7
stress Cobalt chloride 0.4 mM -0.3 +1.0 -1.0 +0.0 -0.3
stress RCH2 defined with Cobalt chloride 0.04 mM -0.3 -0.6 -0.4 +0.4 +0.7
nitrogen source L-Serine (N) -0.5 -1.0 +0.5 +0.6 +0.1
stress deoxycholate 0.0125 M +1.1 +1.1 -0.5 -1.8 +0.2
stress tungstate 0.05 M -0.2 -0.8 +0.7 +0.4 +0.2
nitrogen source nitrogen source nitrate 5mM -0.1 +1.0 +0.7 +0.4 -1.4
nitrogen source L-Serine (N) -0.5 +1.0 +0.3 +0.2 -0.4
nitrogen source nitrogen source nitrite 5mM +0.4 +0.9 -0.1 +0.5 -0.6
nitrogen source nitrogen source nitrate 20mM +0.6 +1.0 +0.5 -0.4 -0.2
nitrogen source nitrogen source nitrite 0.5mM -0.0 +1.9 -0.9 +1.1 -0.4
nitrogen source nitrogen source nitrite 5mM -0.1 +1.2 +0.9 +0.9 -0.0
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