Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_1674 and Pf6N2E2_1675 are separated by 30 nucleotidesPf6N2E2_1675 and Pf6N2E2_1676 are separated by 10 nucleotidesPf6N2E2_1676 and Pf6N2E2_1677 are separated by 157 nucleotidesPf6N2E2_1677 and Pf6N2E2_1678 are separated by 121 nucleotides Pf6N2E2_1674 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 1,980,108 to 1,981,190 _1674 Pf6N2E2_1675 - Lipid carrier protein or keto acyl-CoA thiolase Ltp3 (EC 2.3.1.16), at 1,981,221 to 1,982,417 _1675 Pf6N2E2_1676 - conserved protein associated with acetyl-CoA C-acyltransferase, at 1,982,428 to 1,982,868 _1676 Pf6N2E2_1677 - Chalcone synthase (EC 2.3.1.74), at 1,983,026 to 1,984,075 _1677 Pf6N2E2_1678 - Major facilitator family transporter, at 1,984,197 to 1,985,468 _1678
Group Condition Pf6N2E2_1674 Pf6N2E2_1675 Pf6N2E2_1676 Pf6N2E2_1677 Pf6N2E2_1678
stress Bile salts 0.0125 M -0.3 -0.1 -3.0 +0.9 -0.2
nitrogen source Putrescine (N) +1.1 +0.9 +0.9 +0.7 +0.6
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +0.9 +0.9 +0.7 +1.0 +0.7
nitrogen source nitrogen source nitrite 1mM +1.0 +1.0 +0.8 +0.7 +0.7
stress DEANONOate 0.000625 M +1.4 +0.6 +1.1 +0.9 +0.2
nitrogen source Cytosine (N) +1.5 +1.2 +0.2 +0.6 +0.9
nitrogen source Uridine (N) +1.0 +0.9 +0.9 +1.2 +0.6
vitamin No vitamins t2 +1.0 +0.9 +0.6 +1.7 +0.6
denitrifying Nitrate reduction, replicate C +1.3 +1.0 +0.8 +1.0 +0.8
nitrogen source Urea (N) +1.1 +1.2 +0.9 +1.1 +0.7
vitamin With vitamins t2 +1.1 +0.6 +1.3 +1.7 +0.4
stress copper (II) chloride 3 mM +1.3 +1.0 +1.0 +1.0 +0.9
nitrogen source Putrescine (N) +1.2 +1.1 +0.9 +0.9 +1.0
nitrogen source nitrogen source nitrite 1mM +1.2 +1.1 +1.7 +1.1 +0.6
nitrogen source nitrogen source nitrate 5mM +1.5 +1.7 +0.6 +1.4 +0.8
nitrogen source nitrogen source Ammonium chloride 20mM +1.4 +1.5 +1.5 +1.1 +0.6
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C +1.5 +1.6 +1.0 +1.3 +1.3
nitrogen source nitrogen source nitrate 20mM +1.6 +1.6 +2.1 +1.5 +0.8
nitrogen source nitrogen source nitrate 20mM +1.5 +1.6 +2.5 +1.3 +0.7
nitrogen source Cytidine (N) +2.1 +1.6 +1.1 +1.7 +1.2
nitrogen source nitrogen source nitrate 5mM +1.4 +1.6 +2.2 +1.5 +1.1
nitrogen source Cytosine (N) +2.5 +2.4 +1.1 +1.5 +1.1
motility inner cut, LB soft agar motility assay +2.6 +2.5 +1.3 +1.3 +1.2
nitrogen source Cytidine (N) +2.5 +2.3 +1.1 +2.0 +1.7
nitrogen source nitrogen source Ammonium chloride 20mM +2.3 +2.2 +2.2 +1.7 +1.4
motility inner cut, LB soft agar motility assay +2.6 +2.5 +2.0 +2.0 +1.4
motility inner cut, LB soft agar motility assay +2.6 +2.5 +2.6 +1.8 +1.3
stress periodate 0.00125 M +3.4 +3.0 +1.0 +3.4 +0.3
motility outer cut, LB soft agar motility assay +2.6 +3.1 +2.3 +1.9 +1.4
motility outer cut, LB soft agar motility assay +3.1 +3.1 +2.6 +1.9 +1.2
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