Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_1431 and Pf6N2E2_1432 are separated by 11 nucleotidesPf6N2E2_1432 and Pf6N2E2_1433 are separated by 67 nucleotidesPf6N2E2_1433 and Pf6N2E2_1434 are separated by 13 nucleotidesPf6N2E2_1434 and Pf6N2E2_1435 are separated by 14 nucleotides Pf6N2E2_1431 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 1,680,698 to 1,681,765 _1431 Pf6N2E2_1432 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 1,681,777 to 1,682,658 _1432 Pf6N2E2_1433 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 1,682,726 to 1,683,508 _1433 Pf6N2E2_1434 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,683,522 to 1,684,682 _1434 Pf6N2E2_1435 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases; sulfonate monooxygenase, at 1,684,697 to 1,685,794 _1435
Group Condition Pf6N2E2_1431 Pf6N2E2_1432 Pf6N2E2_1433 Pf6N2E2_1434 Pf6N2E2_1435
nitrogen source nitrogen source nitrite 0.5mM -0.7 +0.1 -1.7 -2.9 -0.4
stress Hydroxylamine 0.5 M -4.1 +0.1 +0.5 +0.7 -2.3
vitamin With vitamins t1 -0.1 -0.6 -0.5 -1.8 -0.3
carbon source Ying_sugar7 rep B; time point 2 +0.2 -0.7 -0.9 -0.2 -1.6
carbon source RCH2_defined -0.0 +0.4 -0.7 -1.3 -1.4
motility inner cut, LB soft agar motility assay -2.0 +0.4 -1.2 -0.3 +0.3
stress Bile salts 0.0125 M -0.6 +0.7 -0.2 -2.4 +0.1
stress thiosulfate 0.25 M -0.7 -0.0 -0.2 -1.6 +0.3
stress Manganese (II) chloride tetrahydrate 0.00078125 M +0.4 -0.5 -0.9 -0.5 -0.7
carbon source Ying_OrganicAcid7 rep B; time point 3 +0.4 -0.8 -0.2 -0.3 -1.3
carbon source Ying_Nucleobase14 rep A; time point 3 -0.8 -0.4 -0.1 +0.5 -1.3
stress RCH2 defined with Nickel (II) chloride 0.04 mM +0.0 +0.5 -0.6 -0.3 -1.7
stress Nickel (II) chloride 0.0025 M -0.3 -0.9 +0.7 -0.7 -0.7
vitamin No vitamins t1 +0.5 -0.6 -0.2 -0.6 -1.1
stress sodium iodate 0.005 M -0.8 +0.1 +0.4 +0.0 -1.6
carbon source Ying_all64 rep C; time point 3 +0.5 +0.2 -0.4 -0.7 -1.4
motility outer cut, LB soft agar motility assay +0.6 -0.8 -0.6 +0.1 -1.0
carbon source Ying_AminoAcid20 rep B; time point 2 -0.9 +0.3 +0.6 +0.5 -2.0
carbon source Carbon source D,L-Lactate 10 mM +0.5 -0.1 -1.8 +0.2 -0.4
stress selenate 0.5 M -1.1 -0.1 -0.8 +0.9 -0.6
nitrogen source nitrogen source nitrate 5mM +0.7 -0.5 -0.5 -1.3 +0.3
stress 4-Hydroxyacetophenone 0.00025 M -0.3 +0.0 +0.4 -1.9 +0.5
stress Zinc chloride 0.0000015625 M -1.4 +0.3 +0.6 -0.2 -0.4
motility outer cut, LB soft agar motility assay -0.4 -0.2 +0.5 +0.4 -1.4
nitrogen source nitrogen source nitrite 0.5mM +0.9 -0.4 +0.3 -1.5 -0.4
stress deoxycholate 0.0125 M +0.1 -0.4 +1.1 -1.4 -0.5
lb LB no stress 48 hours -1.3 -0.1 +0.9 +0.2 +0.4
stress bromate 0.05 M -1.4 +0.6 +0.5 +0.8 -0.1
nitrogen source L-Serine (N) -1.1 -0.1 +0.2 +0.5 +1.1
nitrogen source Glucuronamide (N) -0.5 +0.4 +1.1 +0.8 +0.9
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