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Protein
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Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_1305 and Pf6N2E2_1306 are separated by 83 nucleotides
Pf6N2E2_1306 and Pf6N2E2_1307 are separated by 186 nucleotides
Pf6N2E2_1307 and Pf6N2E2_1308 overlap by 4 nucleotides
Pf6N2E2_1308 and Pf6N2E2_1309 are separated by 5 nucleotides
Pf6N2E2_1305 - Homoprotocatechuate degradative operon repressor, at 1,539,894 to 1,540,295
_1305
Pf6N2E2_1306 - Transcriptional activator of 4-hydroxyphenylacetate 3-monooxygenase operon, XylS/AraC family, at 1,540,379 to 1,541,284
_1306
Pf6N2E2_1307 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,541,471 to 1,542,148
_1307
Pf6N2E2_1308 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68) / 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 1,542,145 to 1,542,915
_1308
Pf6N2E2_1309 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (EC 1.2.1.60), at 1,542,921 to 1,544,381
_1309
Group
Condition
Pf6N2E2
_1305
Pf6N2E2
_1306
Pf6N2E2
_1307
Pf6N2E2
_1308
Pf6N2E2
_1309
stress
deoxycholate 0.0125 M
-3.2
-0.2
-0.8
-0.7
-0.1
stress
sodium iodate 0.005 M
-0.8
-0.5
-0.7
-0.8
-0.2
stress
Nickel (II) chloride 1.5 mM
-1.8
-0.0
-0.0
-0.4
-0.6
stress
sodium iodide 0.125 M
-1.0
-0.4
-0.2
-0.6
-0.3
stress
phosphite 0.25 M
-1.1
-0.5
-0.4
-0.0
-0.3
stress
dimethyl sulfone 0.25 M
-0.5
-0.5
+0.1
-1.0
-0.5
motility
outer cut, LB soft agar motility assay
-0.7
-0.3
+0.4
-1.2
-0.5
nitrogen source
Gly-Glu (N)
-1.1
+0.1
-0.9
-0.4
+0.1
lb
LB no stress 48 hours
-1.6
-0.4
-0.4
+0.2
-0.0
stress
pyrophosphate 0.0025 M
-0.8
-0.5
-0.2
-0.4
-0.4
nitrogen source
nitrogen source nitrite 0.5mM
-1.1
+0.2
-0.4
-0.4
-0.3
stress
DEANONOate 0.000625 M
-1.0
-0.4
-0.4
-0.5
+0.2
stress
Manganese (II) chloride tetrahydrate 0.00078125 M
-1.6
+0.1
+0.1
-0.7
+0.4
carbon source
Carbon source Sucrose 10 mM
-0.5
-0.5
+0.4
-0.2
-0.8
stress
Hans 100x vitamins
-1.1
-0.4
+0.2
-0.5
+0.2
stress
Cobalt chloride 0.4 mM
-1.2
-0.2
+0.2
+0.3
-0.4
stress
Nickel (II) chloride 0.0025 M
-0.7
+0.0
-0.3
+0.5
-0.6
nitrogen source
nitrogen source nitrite 5mM
-0.2
+0.4
-0.4
-1.1
+0.3
stress
thiosulfate 0.25 M
-0.6
-0.3
-0.6
+0.6
-0.1
stress
bromate 0.05 M
-0.1
-0.8
+0.2
-0.6
+0.4
nitrogen source
Gly-Glu (N)
-1.2
+0.2
+0.1
+0.4
-0.3
motility
inner cut, LB soft agar motility assay
+0.3
-0.1
-0.4
+0.7
-0.9
vitamin
No vitamins t1
+0.9
-0.4
+0.3
-0.5
-0.3
stress
Zinc chloride 0.0000015625 M
-0.9
-0.3
+0.4
-0.1
+1.0
vitamin
No vitamins t2
+0.8
-0.2
+0.4
-0.6
-0.1
carbon source
D-Galactose (C)
+1.8
-0.6
+0.1
-0.3
-0.3
carbon source
D-Glucosamine Hydrochloride (C)
+1.7
+0.1
-0.3
-0.1
-0.7
stress
Hydroxylamine 0.5 M
+0.7
+0.3
+0.7
-0.4
-0.4
carbon source
D-Galactose (C)
+2.3
-0.3
+0.1
-0.1
-0.5
carbon source
D-Fructose (C)
+2.9
+0.2
-0.7
+0.0
-0.0
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