Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_1228 and Pf6N2E2_1229 are separated by 169 nucleotidesPf6N2E2_1229 and Pf6N2E2_1230 are separated by 68 nucleotidesPf6N2E2_1230 and Pf6N2E2_1231 overlap by 7 nucleotidesPf6N2E2_1231 and Pf6N2E2_1232 are separated by 14 nucleotides Pf6N2E2_1228 - Lipase (EC 3.1.1.3), at 1,436,712 to 1,438,565 _1228 Pf6N2E2_1229 - Serine protease homologue, at 1,438,735 to 1,441,827 _1229 Pf6N2E2_1230 - Ferric iron ABC transporter, permease protein, at 1,441,896 to 1,442,084 _1230 Pf6N2E2_1231 - ABC-type protease exporter, outer membrane component PrtF/AprF, at 1,442,078 to 1,443,427 _1231 Pf6N2E2_1232 - ABC-type protease exporter, membrane fusion protein (MFP) family component PrtE/AprE, at 1,443,442 to 1,444,758 _1232
Group Condition Pf6N2E2_1228 Pf6N2E2_1229 Pf6N2E2_1230 Pf6N2E2_1231 Pf6N2E2_1232
nitrogen source L-Glutamic (N) -0.2 +0.1 N.D. -0.1 -0.6
carbon source Carbon source D-Glucose 10 mM -0.0 +0.1 N.D. -0.7 -0.1
stress D-Cycloserine 0.12 mg/ml -0.3 -0.2 N.D. -0.2 +0.1
lb LB no stress 48 hours +0.2 -0.2 N.D. -0.2 -0.3
carbon source octanoate (C) +0.1 +0.1 N.D. -0.4 -0.3
carbon source D-Glucosamine Hydrochloride (C) +0.1 -0.3 N.D. +0.1 -0.4
nitrogen source nitrogen source nitrate 20mM -0.0 -0.1 N.D. +0.5 -0.7
carbon source Carbon source D,L-Lactate 10 mM +0.1 +0.1 N.D. -0.4 -0.3
stress Bile salts 0.0125 M +0.2 -0.5 N.D. -0.5 +0.4
stress DEANONOate 0.000625 M -0.2 +0.0 N.D. -0.4 +0.3
nitrogen source Glucuronamide (N) -0.3 -0.1 N.D. +0.4 -0.2
nitrogen source L-Aspartic Acid (N) +0.0 -0.3 N.D. -0.3 +0.4
stress selenate 0.5 M +0.4 +0.0 N.D. -0.4 -0.1
nitrogen source nitrogen source nitrite 5mM -0.4 +0.1 N.D. -0.0 +0.3
stress Lomefloxacin 0.0002 mM +0.3 -0.1 N.D. -0.5 +0.2
stress Zinc chloride 0.0000015625 M -0.1 +0.1 N.D. -0.5 +0.4
stress Nickel (II) chloride 0.0025 M +0.1 -0.2 N.D. +0.4 -0.3
stress Fluorophosphate 0.125 M +0.4 +0.1 N.D. +0.0 -0.4
lb LB no stress 48 hours +0.3 +0.2 N.D. -0.2 -0.2
motility inner cut, LB soft agar motility assay +0.3 -0.1 N.D. -0.3 +0.2
motility outer cut, LB soft agar motility assay +0.1 +0.3 N.D. +0.1 -0.4
carbon source L-Leucine (C) +0.3 -0.4 N.D. +0.2 +0.1
vitamin With vitamins t2 +0.5 +0.3 N.D. -0.2 -0.3
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -0.1 -0.2 N.D. +0.4 +0.2
vitamin With vitamins t1 +0.5 +0.2 N.D. -0.1 -0.3
motility outer cut, LB soft agar motility assay +0.2 +0.1 N.D. -0.3 +0.5
vitamin No vitamins t2 +0.2 +0.3 N.D. -0.3 +0.2
stress deoxycholate 0.0125 M +0.4 -0.2 N.D. +0.3 +0.3
stress Cobalt chloride 0.6 mM +0.2 -0.0 N.D. +0.2 +0.4
vitamin No vitamins t1 +0.3 +0.3 N.D. +0.1 +0.3
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Pf6N2E2_1228
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