Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_1211 and Pf6N2E2_1212 are separated by 187 nucleotidesPf6N2E2_1212 and Pf6N2E2_1213 are separated by 79 nucleotidesPf6N2E2_1213 and Pf6N2E2_1214 are separated by 9 nucleotidesPf6N2E2_1214 and Pf6N2E2_1215 are separated by 168 nucleotides Pf6N2E2_1211 - Lactoylglutathione lyase, at 1,418,143 to 1,418,547 _1211 Pf6N2E2_1212 - Oxidoreductase, short chain dehydrogenase/reductase family, at 1,418,735 to 1,419,592 _1212 Pf6N2E2_1213 - hypothetical protein, at 1,419,672 to 1,419,794 _1213 Pf6N2E2_1214 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 1,419,804 to 1,420,304 _1214 Pf6N2E2_1215 - probable membrane protein YPO2362, at 1,420,473 to 1,421,084 _1215
Group Condition Pf6N2E2_1211 Pf6N2E2_1212 Pf6N2E2_1213 Pf6N2E2_1214 Pf6N2E2_1215
stress deoxycholate 0.0125 M -0.5 -0.5 -0.5 N.D. -3.7
nitrogen source L-Serine (N) -0.6 +0.6 -1.0 N.D. -1.9
stress Fusidic 0.4 mg/ml -0.8 -0.2 -0.6 N.D. -1.1
stress Nickel (II) chloride 1 mM -1.3 +0.2 -0.8 N.D. -0.5
stress dimethyl sulfone 0.25 M -1.6 +0.3 +0.5 N.D. -1.2
nitrogen source nitrogen source nitrate 20mM -0.4 +0.3 -0.7 N.D. -1.0
stress Bile salts 0.0125 M +0.4 -1.1 -0.7 N.D. -0.3
lb LB no stress 48 hours +0.5 -1.1 -0.1 N.D. -1.0
carbon source D-Xylose (C) -1.3 +0.2 -0.6 N.D. +0.3
vitamin With vitamins t1 -2.0 -0.4 +0.1 N.D. +0.9
denitrifying Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C -1.8 +0.2 +0.1 N.D. +0.2
stress Nalidixic 0.004 mg/ml -2.8 +0.7 +0.1 N.D. +0.8
stress Cobalt chloride 0.6 mM -0.9 +0.2 +0.4 N.D. -0.9
nitrogen source nitrogen source nitrite 5mM +1.0 -0.1 -0.2 N.D. -1.7
stress Chloride 600 mM +0.3 -0.4 +0.4 N.D. -1.1
carbon source D-Galactose (C) -1.2 +0.2 +0.6 N.D. -0.4
stress RCH2 defined with Nickel (II) chloride 0.04 mM -1.4 +0.2 +0.8 N.D. -0.1
nitrogen source L-Serine (N) -1.1 -0.1 +0.9 N.D. +0.4
lb LB no stress 48 hours +0.7 +0.4 +0.5 N.D. -1.1
stress sodium iodate 0.005 M +1.3 +0.2 -0.0 N.D. -0.7
motility inner cut, LB soft agar motility assay -0.8 +0.5 +0.1 N.D. +1.0
vitamin With vitamins t2 -0.5 -0.2 +0.7 N.D. +1.0
carbon source 4-Hydroxybenzoic Acid (C) -0.4 -0.3 +0.7 N.D. +1.0
stress Fluorophosphate 0.125 M +1.2 +0.3 +0.4 N.D. -0.6
vitamin No vitamins t2 -1.0 -0.4 +0.7 N.D. +1.9
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +0.3 -0.4 +0.5 N.D. +1.2
nitrogen source Putrescine (N) +1.1 +0.3 +0.6 N.D. -0.3
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine +0.3 -0.4 +0.9 N.D. +1.0
vitamin No vitamins t1 -0.5 -0.1 +1.3 N.D. +1.1
stress DEANONOate 0.000625 M +0.7 +0.3 +0.9 N.D. +0.5
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