Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_1124 and Pf6N2E2_1125 are separated by 142 nucleotidesPf6N2E2_1125 and Pf6N2E2_1126 are separated by 32 nucleotidesPf6N2E2_1126 and Pf6N2E2_1127 overlap by 4 nucleotidesPf6N2E2_1127 and Pf6N2E2_1128 overlap by 10 nucleotides Pf6N2E2_1124 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,319,963 to 1,320,778 _1124 Pf6N2E2_1125 - Transcriptional regulator, AraC family, at 1,320,921 to 1,321,817 _1125 Pf6N2E2_1126 - Two-component system regulatory protein, at 1,321,850 to 1,322,317 _1126 Pf6N2E2_1127 - Sensory box histidine kinase/response regulator, at 1,322,314 to 1,324,632 _1127 Pf6N2E2_1128 - Two-component response regulator, at 1,324,623 to 1,325,291 _1128
Group Condition Pf6N2E2_1124 Pf6N2E2_1125 Pf6N2E2_1126 Pf6N2E2_1127 Pf6N2E2_1128
stress deoxycholate 0.0125 M -0.1 +0.1 -0.5 +0.3 -3.0
motility outer cut, LB soft agar motility assay -1.5 -0.3 -0.3 +0.2 -1.2
stress Vanillyl Alcohol 0.005 M -2.4 -0.1 -0.4 +0.1 +0.1
motility inner cut, LB soft agar motility assay -1.9 -0.1 -0.1 -0.2 -0.2
stress pyrophosphate 0.0025 M -1.3 -0.0 -0.2 -0.1 -0.8
stress thiosulfate 0.25 M +0.2 +0.0 -1.0 +0.2 -1.7
carbon source D-Trehalose (C) -0.5 -0.7 +0.2 -0.2 -1.0
stress perchlorate 0.125 M -1.4 -0.0 -0.2 +0.0 -0.6
carbon source Carbon source D-Glucose 10 mM -0.7 +0.2 -0.0 -0.2 -1.4
stress Chloride 600 mM +0.3 -0.1 -0.6 -0.2 -1.2
stress dimethyl sulfone 0.25 M -0.8 +0.3 -0.4 +0.1 -1.0
stress periodate 0.00125 M -0.8 -0.4 +0.0 +0.2 -0.7
nitrogen source L-Serine (N) -2.1 +0.1 -0.3 +0.5 +0.2
lb LB no stress 24 hours +0.6 -0.0 -0.5 -0.1 -1.4
nitrogen source nitrate (N) -0.5 +0.4 -0.2 +0.0 -1.2
stress phosphite 0.25 M +0.3 -0.4 +0.4 -0.2 -1.1
stress RCH2 defined with Thallium(I) acetate 0.002 mg/ml +0.8 -0.1 +0.3 +0.3 -2.0
stress Magnesium chloride 0.125 M -0.4 +0.3 +0.5 +0.0 -1.0
motility outer cut, LB soft agar motility assay -0.4 +0.8 +0.4 -0.5 -0.9
stress Hydroxylamine 0.5 M +0.7 +0.2 +0.6 +0.2 -1.9
nitrogen source L-Lysine (N) +1.1 -0.1 -0.2 +0.1 -1.1
carbon source Carbon source D,L-Lactate 10 mM -0.9 +0.3 -0.2 -0.2 +0.8
stress Ceftazidime 0.15 mg/ml +0.5 +0.5 +0.3 -0.0 -0.9
motility inner cut, LB soft agar motility assay +0.7 +0.7 +0.1 +0.2 -1.2
resistance 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +0.8 -0.4 -0.4 -0.1 +0.8
stress Cephalothin 4 mg/ml +0.8 +0.5 +0.3 +0.1 -0.8
carbon source Carbon source Sucrose 2.5 mM +0.5 +0.2 -0.4 -0.2 +1.1
nitrogen source L-Serine (N) +0.8 +0.3 -0.1 -0.3 +0.7
nitrogen source L-Aspartic Acid (N) +1.3 -0.4 +0.4 +0.1 +0.2
lb LB no stress 48 hours +0.7 -0.3 +0.7 +0.4 +0.3
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