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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_1068 and Pf6N2E2_1069 are separated by 294 nucleotides
Pf6N2E2_1069 and Pf6N2E2_1070 are separated by 471 nucleotides
Pf6N2E2_1070 and Pf6N2E2_1071 are separated by 13 nucleotides
Pf6N2E2_1071 and Pf6N2E2_1072 are separated by 23 nucleotides
Pf6N2E2_1068 - outer membrane autotransporter, at 1,245,837 to 1,246,751
_1068
Pf6N2E2_1069 - autotransporter, at 1,247,046 to 1,249,421
_1069
Pf6N2E2_1070 - Endonuclease/Exonuclease/phosphatase family protein, at 1,249,893 to 1,250,693
_1070
Pf6N2E2_1071 - hypothetical protein, at 1,250,707 to 1,250,838
_1071
Pf6N2E2_1072 - LmbE-like protein, at 1,250,862 to 1,251,626
_1072
Group
Condition
Pf6N2E2
_1068
Pf6N2E2
_1069
Pf6N2E2
_1070
Pf6N2E2
_1071
Pf6N2E2
_1072
nitrogen source
Cytosine (N)
-0.2
+0.1
+0.1
N.D.
-2.0
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
+0.1
-0.0
-0.2
N.D.
-1.8
nitrogen source
nitrogen source nitrate 20mM
+0.0
-0.0
-0.2
N.D.
-1.8
vitamin
With vitamins t2
-0.3
-0.2
-0.5
N.D.
-0.7
motility
outer cut, LB soft agar motility assay
-0.5
-0.2
-0.1
N.D.
-0.8
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate A
+0.2
-0.0
-0.1
N.D.
-1.6
nitrogen source
Cytidine (N)
-0.1
+0.1
-0.2
N.D.
-1.3
nitrogen source
Cytidine (N)
-0.3
-0.0
+0.1
N.D.
-1.1
nitrogen source
Cytosine (N)
-0.0
+0.0
+0.0
N.D.
-1.4
vitamin
With vitamins t1
-0.2
-0.1
-0.5
N.D.
-0.5
stress
bromate 0.05 M
-0.4
+0.0
-0.3
N.D.
-0.7
carbon source
Ying_sugar7 rep B; time point 1
-1.3
+0.0
+0.2
N.D.
-0.2
carbon source
Ying_Nucleobase14 rep A; time point 2
-0.7
+0.1
-0.2
N.D.
-0.5
carbon source
RCH2_defined
-0.5
+0.1
-0.4
N.D.
-0.5
carbon source
Ying_Nucleobase14 rep C; time point 2
-0.8
-0.0
+0.1
N.D.
-0.5
carbon source
Ying_Nucleobase14 rep B; time point 2
-0.9
+0.1
+0.1
N.D.
-0.4
carbon source
Ying_AminoAcid20 rep B; time point 2
-0.8
+0.1
-0.3
N.D.
-0.1
nitrogen source
Putrescine (N)
-0.2
+0.2
-0.1
N.D.
-1.1
nitrogen source
L-Isoleucine (N)
-0.1
+0.2
-0.1
N.D.
-1.1
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B
+0.2
-0.1
+0.3
N.D.
-1.3
motility
inner cut, LB soft agar motility assay
-0.0
+0.3
+0.0
N.D.
-1.0
vitamin
No vitamins t1
+0.2
+0.1
-0.9
N.D.
-0.2
nitrogen source
L-Serine (N)
+0.1
+0.2
-0.2
N.D.
-0.8
nitrogen source
Uridine (N)
+0.4
-0.0
-0.2
N.D.
-0.8
vitamin
No vitamins t2
+0.0
+0.4
+0.1
N.D.
-1.1
nitrogen source
nitrogen source Ammonium chloride 20mM
+0.1
+0.2
+0.1
N.D.
-0.9
nitrogen source
L-Serine (N)
+0.5
-0.4
-0.1
N.D.
-0.4
nitrogen source
Glycine (N)
+0.3
+0.1
+0.1
N.D.
-0.7
carbon source
D-Glucose (C)
+0.4
-0.1
+0.3
N.D.
-0.6
carbon source
propionate (C)
+0.6
+0.2
-0.1
N.D.
+0.5
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