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Cofit
Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N2E2
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500 nt
Pf6N2E2_1018 and Pf6N2E2_1019 are separated by 1137 nucleotides
Pf6N2E2_1019 and Pf6N2E2_1020 are separated by 73 nucleotides
Pf6N2E2_1020 and Pf6N2E2_1021 are separated by 397 nucleotides
Pf6N2E2_1021 and Pf6N2E2_1022 are separated by 8 nucleotides
Pf6N2E2_1018 - putative DNA-binding protein, at 1,188,030 to 1,189,055
_1018
Pf6N2E2_1019 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,190,193 to 1,191,206
_1019
Pf6N2E2_1020 - TonB-dependent receptor, at 1,191,280 to 1,193,481
_1020
Pf6N2E2_1021 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,193,879 to 1,194,826
_1021
Pf6N2E2_1022 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,194,835 to 1,196,022
_1022
Group
Condition
Pf6N2E2
_1018
Pf6N2E2
_1019
Pf6N2E2
_1020
Pf6N2E2
_1021
Pf6N2E2
_1022
stress
Bile salts 0.0125 M
+0.1
-0.1
-0.3
-0.5
-1.5
carbon source
Carbon source Sucrose 10 mM
-0.1
-0.1
-0.1
-0.4
-0.7
stress
RCH2 defined with Cobalt chloride 0.02 mM
-0.3
-0.5
+0.1
+0.0
-0.7
stress
sodium iodide 0.125 M
-0.1
+0.1
-0.2
-0.2
-1.0
carbon source
RCH2_defined
-0.5
-0.4
+0.1
+0.1
-0.6
stress
perchlorate 0.125 M
-0.3
-0.1
-0.1
+0.1
-0.8
stress
RCH2 defined with Thallium(I) acetate 0.002 mg/ml
-0.4
-0.5
+0.1
-0.4
-0.0
stress
deoxycholate 0.0125 M
+0.2
-0.6
+0.1
-0.3
-0.6
stress
PTLB purified from Pseudomonas sp. FW300-N2E2 0.05 x
-0.0
+0.0
+0.3
-0.1
-1.2
carbon source
L-Alanine (C)
+0.1
-0.1
+0.1
-0.3
-0.8
nitrogen source
L-Glutamic (N)
-0.1
+0.1
+0.2
-0.3
-0.9
stress
Chloride 0.5 M
+0.3
+0.1
+0.1
-0.3
-0.8
stress
RCH2 defined with copper (II) chloride 0.03 mM
-0.1
-0.6
+0.1
+0.5
-0.2
stress
rubidium chloride 0.25 M
+0.2
+0.3
+0.1
-0.1
-0.6
stress
Nalidixic 0.004 mg/ml
+0.2
+0.2
+0.2
+0.2
-0.9
carbon source
D-Trehalose (C)
+0.1
+0.1
-0.2
-0.4
+0.5
carbon source
octanoate (C)
+0.1
-0.7
+0.1
+0.2
+0.4
stress
Cobalt chloride 0.6 mM
-0.1
-0.3
-0.2
+0.2
+0.6
denitrifying
Nitrate reduction, replicate C
+0.2
+0.1
+0.0
-0.5
+0.6
stress
Hydroxide 0.05 M
-0.0
+0.3
+0.1
-0.5
+0.5
stress
Chloride 600 mM
+0.1
-0.4
+0.0
+0.3
+0.6
carbon source
D-Fructose (C)
+0.0
+0.2
-0.1
-0.3
+0.8
stress
Cadmium chloride hemipentahydrate 0.00005 M
+0.1
+0.3
+0.1
-0.3
+0.6
stress
Lomefloxacin 0.0002 mM
-0.2
+0.3
+0.3
+0.2
+0.4
vitamin
No vitamins t2
+0.3
-0.3
+0.2
+0.4
+0.4
carbon source
propionate (C)
+0.1
+0.3
+0.1
+0.3
+0.6
stress
Zinc chloride 0.0000015625 M
+0.2
+0.3
+0.0
+0.6
+0.5
stress
dimethyl sulfone 0.25 M
+0.2
+0.2
+0.2
+0.2
+0.8
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
+0.5
+0.1
+0.1
-0.0
+0.9
stress
Hydroxylamine 0.5 M
+0.3
+0.6
-0.0
-0.1
+1.4
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