Fitness for 5 genes in Pseudomonas fluorescens FW300-N2E2

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500 ntPf6N2E2_100 and Pf6N2E2_101 are separated by 128 nucleotidesPf6N2E2_101 and Pf6N2E2_102 are separated by 338 nucleotidesPf6N2E2_102 and Pf6N2E2_103 are separated by 355 nucleotidesPf6N2E2_103 and Pf6N2E2_104 overlap by 1 nucleotides Pf6N2E2_100 - membrane protein, putative, at 144,153 to 145,364 _100 Pf6N2E2_101 - CigR (Putative inner membrane protein), at 145,493 to 146,005 _101 Pf6N2E2_102 - sigma-54 dependent transcriptional regulator, at 146,344 to 148,188 _102 Pf6N2E2_103 - Various polyols ABC transporter, ATP-binding component, at 148,544 to 149,638 _103 Pf6N2E2_104 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 149,638 to 150,747 _104
Group Condition Pf6N2E2_100 Pf6N2E2_101 Pf6N2E2_102 Pf6N2E2_103 Pf6N2E2_104
stress Vanillyl Alcohol 0.005 M -0.4 -0.9 -0.4 -0.2 -0.3
carbon source Ying_AminoAcid20 rep C; time point 2 -0.2 -1.4 -0.3 -0.1 +0.1
stress Bile salts 0.0125 M -0.4 -0.6 -0.9 -0.2 +0.2
carbon source Ying_Nucleobase14 rep C; time point 1 -0.1 -1.0 -0.2 -0.4 +0.1
stress Chloride 0.5 M -0.4 -0.3 -0.3 -0.4 -0.2
stress Cobalt chloride 0.6 mM -0.5 -0.3 -0.7 -0.4 +0.1
stress sodium iodate 0.005 M -0.2 -0.8 +0.0 -0.6 +0.1
stress Chlorite 0.00005 M -0.1 -0.7 +0.2 -0.2 -0.3
motility inner cut, LB soft agar motility assay +0.2 -0.8 -0.5 -0.3 +0.2
carbon source Ying_AminoAcid20 rep B; time point 2 -0.2 -0.7 -0.4 +0.3 -0.0
stress RCH2 defined with Cobalt chloride 0.02 mM -0.0 +0.4 -0.3 -0.9 -0.1
stress RCH2 defined with Cobalt chloride 0.04 mM +0.2 +0.3 -0.5 -0.7 -0.1
stress Nickel (II) chloride 0.0025 M -0.4 +0.0 -0.6 +0.3 -0.0
nitrogen source Glucuronamide (N) +0.2 +0.3 +0.0 -1.2 -0.1
nitrogen source L-Arginine (N) -0.0 -0.9 -0.2 +0.2 +0.3
stress RCH2 defined with Cobalt chloride 0.04 mM -0.2 +0.5 +0.2 -0.5 -0.5
nitrogen source nitrogen source nitrite 0.5mM -0.1 +0.5 -0.1 -1.2 +0.4
stress RCH2 defined with Cobalt chloride 0.02 mM +0.2 +0.3 +0.1 -0.5 -0.3
carbon source RCH2_defined +0.3 +0.5 -0.1 -0.8 +0.4
carbon source L-Leucine (C) +0.3 +0.6 +0.1 -0.2 -0.3
stress D-Cycloserine 0.12 mg/ml +0.5 -0.3 +0.0 +0.6 -0.0
resistance 50% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine -0.0 +0.7 -0.3 +0.4 +0.1
resistance 50% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine +0.2 +0.7 -0.1 +0.3 -0.1
stress RCH2 defined with Thallium(I) acetate 0.002 mg/ml +0.2 +0.4 +0.6 -0.3 +0.1
resistance 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine +0.0 +0.8 -0.1 +0.6 -0.2
resistance 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine +0.2 +0.4 -0.1 +0.7 +0.1
resistance 20 mM putrescine +0.1 +1.0 -0.3 +0.5 -0.0
stress deoxycholate 0.0125 M +0.1 +0.6 -0.0 -0.1 +0.7
stress Hydroxylamine 0.5 M -0.1 +0.8 -0.1 +0.8 -0.0
nitrogen source L-Serine (N) -0.2 +0.4 +0.4 +0.6 +0.2
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