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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_912 and Pf1N1B4_913 overlap by 4 nucleotides
Pf1N1B4_913 and Pf1N1B4_914 are separated by 65 nucleotides
Pf1N1B4_914 and Pf1N1B4_915 are separated by 11 nucleotides
Pf1N1B4_915 and Pf1N1B4_916 are separated by 10 nucleotides
Pf1N1B4_912 - Toxin mRNA interferase YgiU, at 1,038,515 to 1,038,667
_912
Pf1N1B4_913 - FIG00958224: hypothetical protein, at 1,038,664 to 1,039,152
_913
Pf1N1B4_914 - ABC-type polar amino acid transport system, ATPase component, at 1,039,218 to 1,039,982
_914
Pf1N1B4_915 - Glutamate Aspartate transport system permease protein GltK (TC 3.A.1.3.4), at 1,039,994 to 1,041,091
_915
Pf1N1B4_916 - Glutamate Aspartate transport system permease protein GltJ (TC 3.A.1.3.4), at 1,041,102 to 1,042,229
_916
Group
Condition
Pf1N1B4
_912
Pf1N1B4
_913
Pf1N1B4
_914
Pf1N1B4
_915
Pf1N1B4
_916
carbon source
carbon source Sucrose 0.5mM
-1.0
-0.1
-1.5
-0.1
-0.5
nitrogen source
D-Alanine (N)
-0.4
-0.1
-0.2
-0.8
-1.0
nitrogen source
L-Leucine (N)
+0.3
-0.2
-0.8
-0.8
-0.9
nitrogen source
L-Isoleucine (N)
+0.3
-0.1
-0.6
-1.1
-0.8
carbon source
L-Isoleucine (C)
-0.1
+0.0
-0.4
-0.9
-0.9
carbon source
L-Leucine (C)
-0.2
+0.2
-0.6
-0.5
-0.8
nitrogen source
L-Valine (N)
-0.2
+0.1
-0.1
-0.5
-0.6
carbon source
carbon source Sucrose 0.25mM
-0.4
+0.4
-1.3
-0.0
-0.1
stress
Cisplatin 0.006 mg/ml
-1.2
-0.2
+0.4
-0.1
-0.3
nitrogen source
Uridine (N)
-1.0
+0.0
-0.3
-0.1
+0.2
carbon source
carbon source Sucrose 0.25mM
-0.5
+0.3
-0.8
-0.1
-0.0
carbon source
L-Arabinose (C)
-0.3
-0.4
-0.6
+0.2
-0.0
carbon source
carbon source Sucrose 5mM
-0.7
-0.3
-0.3
+0.2
+0.1
nitrogen source
nitrogen source nitrite 0.1mM
-0.6
-0.1
-0.4
+0.2
+0.1
carbon source
octanoate (C)
-0.3
-0.1
+0.5
-0.7
+0.0
carbon source
L-Valine (C)
+0.1
-0.4
+0.5
-0.5
-0.2
nitrogen source
nitrate (N)
-0.6
+0.2
-0.3
-0.1
+0.2
anaerobic
N2E2wt with N1B4m denitrifying repB
+0.3
-0.5
+0.4
-0.4
-0.1
stress
Fusidic 0.0625 mg/ml
-0.3
+0.1
+0.4
-0.2
-0.4
stress
Aluminum chloride 2.5 mM
-0.5
+0.1
+0.5
-0.5
+0.1
stress
Cephalothin 2.5 mg/ml
+0.2
-0.6
+0.5
-0.1
-0.1
pH
Minimal media pH9
-0.6
+0.5
+0.2
+0.1
-0.1
carbon source
D-Alanine (C)
+0.4
-0.1
+0.3
-0.5
-0.0
stress
Sisomicin 0.001 mg/ml
+0.4
+0.5
-0.3
-0.1
-0.2
carbon source
carbon source Sucrose 5mM
+0.4
+0.6
-0.3
-0.1
+0.1
carbon source
Cytidine (C)
-0.3
+0.2
+0.9
-0.1
-0.0
stress
Choline Lysine 6 mM
+0.5
+0.6
-0.2
-0.1
+0.1
denitrifying
Nitrate reduction, replicate A t2
+0.1
+0.7
+0.1
-0.2
+0.2
temperature
LB 37C
-0.3
+0.2
+0.9
+0.3
+0.6
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
+0.6
+0.6
+0.3
-0.1
+0.3
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