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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_82 and Pf1N1B4_83 are separated by 53 nucleotides
Pf1N1B4_83 and Pf1N1B4_84 are separated by 250 nucleotides
Pf1N1B4_84 and Pf1N1B4_85 are separated by 130 nucleotides
Pf1N1B4_85 and Pf1N1B4_86 are separated by 29 nucleotides
Pf1N1B4_82 - Mobile element protein, at 85,240 to 86,181
_82
Pf1N1B4_83 - putative lipoprotein, at 86,235 to 86,984
_83
Pf1N1B4_84 - Aldose 1-epimerase (EC 5.1.3.3), at 87,235 to 88,383
_84
Pf1N1B4_85 - Oxidoreductase, aldo/keto reductase family, at 88,514 to 89,335
_85
Pf1N1B4_86 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 89,365 to 89,835
_86
Group
Condition
Pf1N1B4
_82
Pf1N1B4
_83
Pf1N1B4
_84
Pf1N1B4
_85
Pf1N1B4
_86
carbon source
carbon source Sucrose 1mM
N.D.
-0.1
-0.4
-0.9
N.D.
carbon source
carbon source Sucrose 5mM
N.D.
-0.5
-0.0
-0.4
N.D.
anaerobic
Nitrate reduction
N.D.
-0.5
-0.2
-0.2
N.D.
stress
Cisplatin 0.006 mg/ml
N.D.
-0.1
-0.7
-0.1
N.D.
carbon source
carbon source Sucrose 5mM
N.D.
-0.1
-0.6
-0.2
N.D.
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate C; timepoint 1
N.D.
-0.2
-0.4
-0.2
N.D.
nophagecontrol
TIme6-nophage
N.D.
+0.0
-0.1
-0.7
N.D.
denitrifying
Nitrate reduction, replicate C t3
N.D.
-0.1
-0.4
-0.2
N.D.
lb
LB
N.D.
-0.2
-0.5
+0.1
N.D.
carbon source
carbon source Sucrose 1mM
N.D.
+0.2
-0.1
-0.6
N.D.
stress
Choline chloride 200 mM
N.D.
-0.2
+0.2
-0.5
N.D.
lb
LB
N.D.
-0.4
-0.2
+0.2
N.D.
phagefrs
Time16-FRS
N.D.
+0.2
-0.2
-0.3
N.D.
nitrogen source
L-Methionine (N)
N.D.
+0.2
+0.2
-0.7
N.D.
denitrifying
Nitrate reduction, replicate A
N.D.
+0.2
-0.4
-0.0
N.D.
stress
Spectinomycin 0.05 mg/ml
N.D.
+0.3
-0.2
-0.1
N.D.
temperature
LB 37C
N.D.
-0.1
-0.3
+0.3
N.D.
carbon source
carbon source Sucrose 20mM
N.D.
+0.3
-0.4
+0.1
N.D.
lb
LB
N.D.
+0.4
-0.5
+0.2
N.D.
stress
copper (II) chloride 2.5 mM
N.D.
+0.4
+0.2
-0.3
N.D.
stress
Chlorite 1 mM
N.D.
+0.3
-0.2
+0.3
N.D.
nitrogen source
Ammonium chloride (N)
N.D.
+0.2
-0.1
+0.4
N.D.
stress
benzoic 0.58 mg/ml
N.D.
+0.4
-0.3
+0.4
N.D.
denitrifying
Nitrate reduction, replicate B
N.D.
+0.2
-0.1
+0.5
N.D.
stress
Tetracycline 0.0004 mg/ml
N.D.
+0.6
-0.1
+0.2
N.D.
stress
Fusidic 0.0625 mg/ml
N.D.
+0.2
+0.2
+0.3
N.D.
carbon source
D-Galactose (C)
N.D.
+0.3
+0.2
+0.2
N.D.
carbon source
carbon source Sucrose 0.25mM
N.D.
+0.3
+0.2
+0.4
N.D.
denitrifying
Nitrate reduction in presence of wild-type Pseudomonas N2-E2; replicate A
N.D.
+0.1
+0.4
+0.5
N.D.
stress
Doxycycline hyclate 0.0001 mg/ml
N.D.
+0.6
+0.2
+0.3
N.D.
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