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Protein
Homologs
Fitness for 5 genes in
Pseudomonas fluorescens FW300-N1B4
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500 nt
Pf1N1B4_837 and Pf1N1B4_838 are separated by 101 nucleotides
Pf1N1B4_838 and Pf1N1B4_839 are separated by 83 nucleotides
Pf1N1B4_839 and Pf1N1B4_840 are separated by 98 nucleotides
Pf1N1B4_840 and Pf1N1B4_841 are separated by 231 nucleotides
Pf1N1B4_837 - Trehalose operon transcriptional repressor, at 960,712 to 961,416
_837
Pf1N1B4_838 - hypothetical protein, at 961,518 to 966,230
_838
Pf1N1B4_839 - GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2), at 966,314 to 967,891
_839
Pf1N1B4_840 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) / CBS domain, at 967,990 to 969,459
_840
Pf1N1B4_841 - ABC-type sugar transport systems, ATPase components, at 969,691 to 970,239
_841
Group
Condition
Pf1N1B4
_837
Pf1N1B4
_838
Pf1N1B4
_839
Pf1N1B4
_840
Pf1N1B4
_841
carbon source
carbon source Sucrose 0.25mM
N.D.
+0.0
N.D.
N.D.
-1.2
nitrogen source
L-Methionine (N)
N.D.
-0.3
N.D.
N.D.
-0.8
carbon source
pyruvate (C)
N.D.
+0.0
N.D.
N.D.
-1.0
carbon source
Tween 20 (C)
N.D.
-0.0
N.D.
N.D.
-0.9
stress
Sisomicin 0.001 mg/ml
N.D.
+0.1
N.D.
N.D.
-0.9
nitrogen source
Ammonium chloride (N)
N.D.
+0.1
N.D.
N.D.
-0.9
carbon source
a-Ketoglutaric (C)
N.D.
+0.0
N.D.
N.D.
-0.9
temperature
LB 20C
N.D.
-0.1
N.D.
N.D.
-0.7
denitrifying
Nitrate reduction, replicate A t1
N.D.
-0.1
N.D.
N.D.
-0.7
nitrogen source
Uridine (N)
N.D.
-0.2
N.D.
N.D.
-0.5
carbon source
m-Inositol (C)
N.D.
-0.1
N.D.
N.D.
-0.6
anaerobic
N2E2wt with N1B4m denitrifying repB
N.D.
-0.2
N.D.
N.D.
-0.5
carbon source
D-Glucose (C)
N.D.
+0.1
N.D.
N.D.
-0.8
stress
Thallium(I) acetate 0.008 mg/ml
N.D.
+0.0
N.D.
N.D.
-0.7
carbon source
L-Leucine (C)
N.D.
-0.2
N.D.
N.D.
-0.5
stress
Doxycycline hyclate 0.0001 mg/ml
N.D.
-0.2
N.D.
N.D.
-0.5
nitrogen source
nitrogen source nitrate 20mM
N.D.
-0.0
N.D.
N.D.
-0.7
carbon source
L-Glutamic (C)
N.D.
-0.0
N.D.
N.D.
-0.6
stress
Cobalt chloride 0.3125 mM
N.D.
-0.2
N.D.
N.D.
-0.4
stress
Rifampicin 0.004 mg/ml
N.D.
+0.2
N.D.
N.D.
-0.8
nitrogen source
nitrogen source nitrate 5mM
N.D.
-0.1
N.D.
N.D.
-0.5
carbon source
L-Malic (C)
N.D.
-0.1
N.D.
N.D.
-0.5
pH
Minimal media pH9
N.D.
+0.1
N.D.
N.D.
-0.6
stress
Cephalothin 2.5 mg/ml
N.D.
+0.0
N.D.
N.D.
-0.6
stress
Benzethonium chloride 0.0125 mM
N.D.
+0.1
N.D.
N.D.
-0.6
denitrifying
Denitrifying in presence of WT Pseudomonas N2-E3; replicate B; timepoint 1
N.D.
+0.0
N.D.
N.D.
-0.6
nitrogen source
nitrogen source nitrite 0.1mM
N.D.
+0.1
N.D.
N.D.
-0.6
stress
Thallium(I) acetate 0.008 mg/ml
N.D.
+0.2
N.D.
N.D.
-0.5
carbon source
carbon source Sucrose 20mM
N.D.
+0.2
N.D.
N.D.
-0.4
carbon source
carbon source Sucrose 5mM
N.D.
+0.2
N.D.
N.D.
+1.1
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